scholarly journals In vivo photoacoustic and ultrasonic mapping of rat sentinel lymph nodes with a modified commercial ultrasound imaging system

2010 ◽  
Author(s):  
Todd N. Erpelding ◽  
Chulhong Kim ◽  
Manojit Pramanik ◽  
Zijian Guo ◽  
John Dean ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Ultrasound Imaging ◽  
Imaging System ◽  
Sentinel Lymph Nodes

scholarly journals A Tumbling Magnetic Microrobot System for Biomedical Applications

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 861
Author(s):  
Elizabeth E. Niedert ◽  
Chenghao Bi ◽  
Georges Adam ◽  
Elly Lambert ◽  
Luis Solorio ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Biomedical Applications ◽  
Imaging System ◽  
Ex Vivo ◽  
Negative Control ◽  
Circular Path ◽  
Rotational Axis ◽  
Significant Difference

A microrobot system comprising an untethered tumbling magnetic microrobot, a two-degree-of-freedom rotating permanent magnet, and an ultrasound imaging system has been developed for in vitro and in vivo biomedical applications. The microrobot tumbles end-over-end in a net forward motion due to applied magnetic torque from the rotating magnet. By turning the rotational axis of the magnet, two-dimensional directional control is possible and the microrobot was steered along various trajectories, including a circular path and P-shaped path. The microrobot is capable of moving over the unstructured terrain within a murine colon in in vitro, in situ, and in vivo conditions, as well as a porcine colon in ex vivo conditions. High-frequency ultrasound imaging allows for real-time determination of the microrobot’s position while it is optically occluded by animal tissue. When coated with a fluorescein payload, the microrobot was shown to release the majority of the payload over a 1-h time period in phosphate-buffered saline. Cytotoxicity tests demonstrated that the microrobot’s constituent materials, SU-8 and polydimethylsiloxane (PDMS), did not show a statistically significant difference in toxicity to murine fibroblasts from the negative control, even when the materials were doped with magnetic neodymium microparticles. The microrobot system’s capabilities make it promising for targeted drug delivery and other in vivo biomedical applications.

scholarly journals Noninvasive photoacoustic identification of sentinel lymph nodes containing methylene blue in vivo in a rat model

2008 ◽  
Vol 13 (5) ◽  
pp. 054033 ◽  
Author(s):  
Kwang Hyun Song ◽  
Erich W. Stein ◽  
Julie A. Margenthaler ◽  
Lihong V. Wang
Keyword(s):  
Methylene Blue ◽  
Lymph Nodes ◽  
Rat Model ◽  
Sentinel Lymph Nodes

Contrast-enhanced lymphatic ultrasound imaging (lymphosonography) of sentinel lymph nodes in a melanoma animal model

2003 ◽  
Vol 29 (5) ◽  
pp. S95-S96
Author(s):  
B.B. Goldberg ◽  
D.A. Merton ◽  
J. Liu ◽  
M. Thakur ◽  
G.F. Murphy ◽  
...  
Keyword(s):  
Animal Model ◽  
Lymph Nodes ◽  
Ultrasound Imaging ◽  
Sentinel Lymph Nodes ◽  
Contrast Enhanced

scholarly journals Paclitaxel induces lymphatic endothelial cells autophagy to promote metastasis

2019 ◽  
Vol 10 (12) ◽  
Author(s):  
Audrey Zamora ◽  
Melinda Alves ◽  
Charlotte Chollet ◽  
Nicole Therville ◽  
Tiffany Fougeray ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Lymphatic System ◽  
Locally Advanced Breast Cancer ◽  
Locally Advanced ◽  
Sentinel Lymph Nodes ◽  
Cancer Drug ◽  
Lymphatic Endothelium ◽  
Primary Tumors

AbstractCytotoxic therapy for breast cancer inhibits the growth of primary tumors, but promotes metastasis to the sentinel lymph nodes through the lymphatic system. However, the effect of first-line chemotherapy on the lymphatic endothelium has been poorly investigated. In this study, we determined that paclitaxel, the anti-cancer drug approved for the treatment of metastatic or locally advanced breast cancer, induces lymphatic endothelial cell (LEC) autophagy to increase metastases. While paclitaxel treatment was largely efficacious in inhibiting LEC adhesion, it had no effect on cell survival. Paclitaxel inhibited LEC migration and branch point formation by inducing an autophagy mechanism independent of Akt phosphorylation. In vivo, paclitaxel mediated a higher permeability of lymphatic endothelium to tumor cells and this effect was reversed by chloroquine, an autophagy-lysosome inhibitor. Despite a strong effect on reducing tumor size, paclitaxel significantly increased metastasis to the sentinel lymph nodes. This effect was restricted to a lymphatic dissemination, as chemotherapy did not affect the blood endothelium. Taken together, our findings suggest that the lymphatic system resists to chemotherapy through an autophagy mechanism to promote malignant progression and metastatic lesions. This study paves the way for new combinative therapies aimed at reducing the number of metastases.

scholarly journals In Vivo Dual-Modal Photoacoustic and Ultrasound Imaging of Sentinel Lymph Nodes Using a Solid-State Dye Laser System

Sensors ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 3714
Author(s):  
Moongyu Han ◽  
Wonseok Choi ◽  
Joongho Ahn ◽  
Hanyoung Ryu ◽  
Youngseok Seo ◽  
...  
Keyword(s):  
Solid State ◽  
Ultrasound Imaging ◽  
Imaging System ◽  
Laser System ◽  
Thick Layer ◽  
Dye Laser ◽  
Chicken Breast ◽  
Breast Cancer Patients ◽  
Solid State Dye Laser

Photoacoustic imaging (PAI) is being actively investigated as a non-invasive and non-radioactive imaging technique for sentinel lymph node (SLN) biopsy. By taking advantage of optical and ultrasound imaging, PAI probes SLNs non-invasively with methylene blue (MB) in both live animals and breast cancer patients. However, these PAI systems have limitations for widespread use in clinics and commercial marketplaces because the lasers used by the PAI systems, e.g., tunable liquid dye laser systems and optical parametric oscillator (OPO) lasers, are bulky in size, not economical, and use risky flammable and toxic liquid dyes. To overcome these limitations, we are proposing a novel dual-modal photoacoustic and ultrasound imaging system based on a solid-state dye laser (SD-PAUSI), which is compact, convenient, and carries far less risk of flammability and toxicity. Using a solid-state dye handpiece that generates 650-nm wavelength, we successfully imaged the MB tube positioned deeply (~3.9 cm) in chicken breast tissue. The SLNs were also photoacoustically detected in the in vivo rats beneath a 2.2-cm-thick layer of chicken breast, which is deeper than the typical depth of SLNs in humans (1.2 ± 0.5 cm). Furthermore, we showed the multispectral capability of the PAI by switching the dye handpiece, in which the MB-dyed SLN was selectively highlighted from the surrounding vasculature. These results demonstrated the great potential of the SD-PAUSI as an easy but effective modality for SLN detection.

Evaluation of Nodal Coverage for Cervical Cancer by In Vivo Localization of Sentinel Lymph Nodes

2009 ◽  
Vol 75 (3) ◽  
pp. S149
Author(s):  
A.K. Bhatt ◽  
G.M. Cannon ◽  
D.M. Kushner ◽  
M.A. Wilson ◽  
K.A. Bradley
Keyword(s):  
Cervical Cancer ◽  
Lymph Nodes ◽  
Sentinel Lymph Nodes

Sentinel lymph nodes detection with an imaging system using Patent Blue V dye as fluorescent tracer

2013 ◽  
Author(s):  
F. Tellier ◽  
J. Steibel ◽  
R. Chabrier ◽  
J. F. Rodier ◽  
G. Pourroy ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Imaging System ◽  
Sentinel Lymph Nodes ◽  
Fluorescent Tracer ◽  
Patent Blue ◽  
Patent Blue V
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