scholarly journals In Vivo Dual-Modal Photoacoustic and Ultrasound Imaging of Sentinel Lymph Nodes Using a Solid-State Dye Laser System

Sensors ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 3714
Author(s):  
Moongyu Han ◽  
Wonseok Choi ◽  
Joongho Ahn ◽  
Hanyoung Ryu ◽  
Youngseok Seo ◽  
...  
Keyword(s):  
Solid State ◽  
Ultrasound Imaging ◽  
Imaging System ◽  
Laser System ◽  
Thick Layer ◽  
Dye Laser ◽  
Chicken Breast ◽  
Breast Cancer Patients ◽  
Solid State Dye Laser

Photoacoustic imaging (PAI) is being actively investigated as a non-invasive and non-radioactive imaging technique for sentinel lymph node (SLN) biopsy. By taking advantage of optical and ultrasound imaging, PAI probes SLNs non-invasively with methylene blue (MB) in both live animals and breast cancer patients. However, these PAI systems have limitations for widespread use in clinics and commercial marketplaces because the lasers used by the PAI systems, e.g., tunable liquid dye laser systems and optical parametric oscillator (OPO) lasers, are bulky in size, not economical, and use risky flammable and toxic liquid dyes. To overcome these limitations, we are proposing a novel dual-modal photoacoustic and ultrasound imaging system based on a solid-state dye laser (SD-PAUSI), which is compact, convenient, and carries far less risk of flammability and toxicity. Using a solid-state dye handpiece that generates 650-nm wavelength, we successfully imaged the MB tube positioned deeply (~3.9 cm) in chicken breast tissue. The SLNs were also photoacoustically detected in the in vivo rats beneath a 2.2-cm-thick layer of chicken breast, which is deeper than the typical depth of SLNs in humans (1.2 ± 0.5 cm). Furthermore, we showed the multispectral capability of the PAI by switching the dye handpiece, in which the MB-dyed SLN was selectively highlighted from the surrounding vasculature. These results demonstrated the great potential of the SD-PAUSI as an easy but effective modality for SLN detection.

scholarly journals Performance benchmarks of an array-based hand-held photoacoustic probe adapted from a clinical ultrasound system for non-invasive sentinel lymph node imaging

2011 ◽  
Vol 369 (1955) ◽  
pp. 4644-4650 ◽  
Author(s):  
Chulhong Kim ◽  
Todd N. Erpelding ◽  
Ladislav Jankovic ◽  
Lihong V. Wang
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Ex Vivo ◽  
Thick Layer ◽  
Chicken Breast ◽  
Breast Cancer Patients ◽  
Non Invasive ◽  
Lymph Node Imaging ◽  
Chicken Tissue

Clinical translation of photoacoustic (PA) imaging can be facilitated by integration with commercial ultrasound (US) scanners to enable dual-modality imaging. An array-based US scanner was modified for hand-held PA imaging. The performance was benchmarked in terms of signal-to-noise ratio (SNR), axial spatial resolution and sensitivity. PA images of a tube, filled with methylene blue (MB; approx. 30 mM) and placed at various depths in chicken tissue, were acquired. A 5 cm penetration depth was achieved with an 18.6 dB SNR using a laser fluence of 3 mJ cm −2 , only one-seventh of the safety limit (20 mJ cm −2 ). An axial resolution of approximately 400 μm was maintained at all imaging depths. The PA sensitivity to MB placed 2.3 cm deep in chicken tissue was less than 100 μM. Further, after intradermal injection of MB (approx. 30 mM), a rat sentinel lymph node was clearly identified in vivo , beneath a 3.8 cm thick layer of chicken breast. The accumulated concentration of MB in the node was estimated to be approximately 7 mM. The noise-equivalent sensitivities (approx. 2 cm depth) were 17 and 85 μM, ex vivo and in vivo , respectively. These results support the use of this PA system for non-invasive mapping and image-guided needle biopsy of sentinel nodes in breast cancer patients.

SOLID-STATE DYE LASER WITH DISPERSIVE RESONATOR

2016 ◽  
Vol 75 (3) ◽  
pp. 255-263
Author(s):  
M. I. Dzyubenko ◽  
S. V. Nikolaev ◽  
V. V. Pozhar ◽  
K. S. Nikolaev
Keyword(s):  
Solid State ◽  
Dye Laser ◽  
Solid State Dye Laser

scholarly journals A Tumbling Magnetic Microrobot System for Biomedical Applications

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 861
Author(s):  
Elizabeth E. Niedert ◽  
Chenghao Bi ◽  
Georges Adam ◽  
Elly Lambert ◽  
Luis Solorio ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Biomedical Applications ◽  
Imaging System ◽  
Ex Vivo ◽  
Negative Control ◽  
Circular Path ◽  
Rotational Axis ◽  
Significant Difference

A microrobot system comprising an untethered tumbling magnetic microrobot, a two-degree-of-freedom rotating permanent magnet, and an ultrasound imaging system has been developed for in vitro and in vivo biomedical applications. The microrobot tumbles end-over-end in a net forward motion due to applied magnetic torque from the rotating magnet. By turning the rotational axis of the magnet, two-dimensional directional control is possible and the microrobot was steered along various trajectories, including a circular path and P-shaped path. The microrobot is capable of moving over the unstructured terrain within a murine colon in in vitro, in situ, and in vivo conditions, as well as a porcine colon in ex vivo conditions. High-frequency ultrasound imaging allows for real-time determination of the microrobot’s position while it is optically occluded by animal tissue. When coated with a fluorescein payload, the microrobot was shown to release the majority of the payload over a 1-h time period in phosphate-buffered saline. Cytotoxicity tests demonstrated that the microrobot’s constituent materials, SU-8 and polydimethylsiloxane (PDMS), did not show a statistically significant difference in toxicity to murine fibroblasts from the negative control, even when the materials were doped with magnetic neodymium microparticles. The microrobot system’s capabilities make it promising for targeted drug delivery and other in vivo biomedical applications.

Solid state dye laser

1995 ◽  
Author(s):  
Igor K. Meshkovskii ◽  
Vladimir I. Zemskii ◽  
Yuri L. Kolesnikov
Keyword(s):  
Solid State ◽  
Dye Laser ◽  
Solid State Dye Laser

scholarly journals Mode suppression in a microcavity solid-state dye laser

2007 ◽  
Vol 2 ◽  
Author(s):  
Sergei Popov ◽  
Sebastien Ricciardi ◽  
Ari T. Friberg ◽  
Sergey Sergeyev
Keyword(s):  
Solid State ◽  
Dye Laser ◽  
Mode Suppression ◽  
Solid State Dye Laser

Photopumped Organic Solid-State Dye Laser with a Second-Order Distributed Feedback Cavity

2001 ◽  
Vol 40 (Part 2, No. 8A) ◽  
pp. L799-L801 ◽  
Author(s):  
Musubu Ichikawa ◽  
Yuji Tanaka ◽  
Naotoshi Suganuma ◽  
Toshiki Koyama ◽  
Yoshio Taniguchi
Keyword(s):  
Solid State ◽  
Dye Laser ◽  
Second Order ◽  
Distributed Feedback ◽  
Organic Solid ◽  
Solid State Dye Laser ◽  
Organic Solid State

Single Longitudinal Mode Operation of a Solid-State Dye Laser Oscillator

2000 ◽  
Vol 37 (5) ◽  
pp. 783-787 ◽  
Author(s):  
Gwon Lim ◽  
Do-Kyeong Ko ◽  
Hyun Su Kim ◽  
Byung Heon Cha ◽  
Jongmin Lee
Keyword(s):  
Solid State ◽  
Longitudinal Mode ◽  
Dye Laser ◽  
Single Longitudinal Mode ◽  
Laser Oscillator ◽  
Mode Operation ◽  
Solid State Dye Laser

Far-red polyurethane-host solid-state dye laser

2009 ◽  
Vol 39 (9) ◽  
pp. 789-792 ◽  
Author(s):  
S V Nikolaev ◽  
V V Pozhar ◽  
M I Dzyubenko ◽  
A O Doroshenko
Keyword(s):  
Solid State ◽  
Dye Laser ◽  
Solid State Dye Laser
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