In vivo trans-rectal ultrasound coupled trans-rectal near-infrared optical tomography of canine prostate bearing transmissible venereal tumor

2009 ◽  
Author(s):  
Zhen Jiang ◽  
G. Reed Holyoak ◽  
Kenneth E. Bartels ◽  
Jerry W. Ritchey ◽  
Guan Xu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Optical Tomography ◽  
Rectal Ultrasound

IN VIVO TRANS-RECTAL ULTRASOUND-COUPLED NEAR-INFRARED OPTICAL TOMOGRAPHY OF INTACT NORMAL CANINE PROSTATE

2009 ◽  
Vol 02 (03) ◽  
pp. 215-225 ◽  
Author(s):  
DAQING PIAO ◽  
ZHEN JIANG ◽  
KENNETH E. BARTELS ◽  
G. REED HOLYOAK ◽  
JERRY W. RITCHEY ◽  
...  
Keyword(s):  
Near Infrared ◽  
Prostate Gland ◽  
Optical Tomography ◽  
Left Lobe ◽  
Attenuation Coefficients ◽  
Scattering Coefficients ◽  
Rectal Ultrasound ◽  
State Measurement ◽  
The Right

This is the first tomography-presentation of the optical properties of a normal canine prostate, in vivo, in its native intact environment in the pelvic canal. The imaging was performed by trans-rectal near-infrared (NIR) optical tomography in steady-state measurement at 840 nm on three sagittal planes across the right lobe, middle-line, and left lobe, respectively, of the prostate gland. The NIR imaging planes were position-correlated with concurrently applied trans-rectal ultrasound, albeit there was no spatial prior employed in the NIR tomography reconstruction. The reconstructed peak absorption coefficients of the prostate on the three planes were 0.014, 0.012, and 0.014 mm-1. The peak reduced scattering coefficients were 5.28, 5.56, and 6.53 mm-1. The peak effective attenuation coefficients were 0.45, 0.43, and 0.50 mm-1. The absorption and effective attenuation coefficients were within the ranges predictable at 840 nm by literature values which clustered sparsely from 355 nm to 1064 nm, none of which were performed on a canine prostate with similar conditions. The effective attenuation coefficients of the gland were shown to be generally higher in the internal aspects than in the peripheral aspects, which is consistent with the previous findings that the urethral regions were statistically more attenuating than the capsular regions.

scholarly journals Trans-rectal ultrasound-coupled near-infrared optical tomography of the prostate, Part I: Simulation

2008 ◽  
Vol 16 (22) ◽  
pp. 17484 ◽  
Author(s):  
Guan Xu ◽  
Daqing Piao ◽  
Cameron H. Musgrove ◽  
Charles F. Bunting ◽  
Hamid Dehghani
Keyword(s):  
Near Infrared ◽  
Optical Tomography ◽  
Rectal Ultrasound

scholarly journals In vivo trans-rectal ultrasound–coupled optical tomography of a transmissible venereal tumor model in the canine pelvic canal

2009 ◽  
Vol 14 (3) ◽  
pp. 030506 ◽  
Author(s):  
Zhen Jiang ◽  
G. Reed Holyoak ◽  
Kenneth E. Bartels ◽  
Jerry W. Ritchey ◽  
Guan Xu ◽  
...  
Keyword(s):  
Optical Tomography ◽  
Tumor Model ◽  
Rectal Ultrasound

In vivo imaging of small animals with optical tomography and near-infrared fluorescent probes

2002 ◽  
Author(s):  
Matthew R. Palmer ◽  
Yasushi Shibata ◽  
Jonathan B. Kruskal ◽  
Robert E. Lenkinski
Keyword(s):  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Optical Tomography ◽  
Small Animals

scholarly journals Trans-rectal ultrasound-coupled near-infrared optical tomography of the prostate, Part II: Experimental demonstration

2008 ◽  
Vol 16 (22) ◽  
pp. 17505 ◽  
Author(s):  
Zhen Jiang ◽  
Daqing Piao ◽  
Guan Xu ◽  
Jerry W. Ritchey ◽  
G. R. Holyoak ◽  
...  
Keyword(s):  
Near Infrared ◽  
Optical Tomography ◽  
Experimental Demonstration ◽  
Rectal Ultrasound

scholarly journals In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes

2018 ◽  
Author(s):  
Sez-Jade Chen ◽  
Nattawut Sinsuebphon ◽  
Alena Rudkouskaya ◽  
Margarida Barroso ◽  
Xavier Intes ◽  
...  
Keyword(s):  
Near Infrared ◽  
Specific Binding ◽  
Optical Tomography ◽  
Dynamic Imaging ◽  
Model Systems ◽  
Computationally Efficient ◽  
Time Resolved ◽  
Phasor Analysis

1AbstractWe introduce a simple new approach for time-resolved multiplexed analysis of complex systems using near-infrared (NIR) dyes, applicable to in vitro and in vivo studies. We first show that fast and precise in vitro quantification of NIR fluorophores lifetime and stoichiometry can be done using phasor analysis, a computationally efficient and user-friendly representation of complex fluorescence intensity decays obtained with pulsed laser excitation. We apply this approach to the study of binding equilibria by Förster resonant energy transfer (FRET), using two different model systems: primary/secondary antibody binding in vitro and ligand/receptor binding in cell cultures. We then extend our demonstration to dynamic imaging of the pharmacokinetics of transferrin binding to the transferrin receptor in live mice, elucidating the kinetic of differential transferrin accumulation in specific organs, straightforwardly differentiating specific from non-specific binding. Our method, implemented in a freely-available software package, has all the advantages of time-resolved NIR imaging, including better tissue penetration and background-free imaging, but simplifies and considerably speeds up data processing and interpretation, while remaining quantitative. These advances make this method attractive and of broad applicability for in vitro and in vivo molecular imaging, and could be extended to applications as diverse as image guided-surgery or optical tomography.

scholarly journals Near-Infrared Fluorescence-Enhanced Optical Tomography

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Banghe Zhu ◽  
Anuradha Godavarty
Keyword(s):  
Distributed Memory ◽  
Near Infrared ◽  
State Of The Art ◽  
Optical Tomography ◽  
Forward Modeling ◽  
Molecular Targeting ◽  
Nir Light ◽  
Current State ◽  
Nir Fluorescence

Fluorescence-enhanced optical imaging using near-infrared (NIR) light developed forin vivomolecular targeting and reporting of cancer provides promising opportunities for diagnostic imaging. The current state of the art of NIR fluorescence-enhanced optical tomography is reviewed in the context of the principle of fluorescence, the different measurement schemes employed, and the mathematical tools established to tomographically reconstruct the fluorescence optical properties in various tissue domains. Finally, we discuss the recent advances in forward modeling and distributed memory parallel computation to provide robust, accurate, and fast fluorescence-enhanced optical tomography.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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