In-vivo corneal nonlinear optical tomography based on second harmonic and multiphoton autofluorescence imaging induced by near-infrared femtosecond lasers with rabbits

2005 ◽  
Author(s):  
Baogui Wang ◽  
Karl-Juergen Halbhuber ◽  
Iris Riemann ◽  
Karsten Koenig
Keyword(s):  
Nonlinear Optical ◽  
Near Infrared ◽  
Femtosecond Lasers ◽  
Second Harmonic ◽  
Optical Tomography ◽  
Autofluorescence Imaging

scholarly journals Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1105
Author(s):  
Pálma Anker ◽  
Luca Fésűs ◽  
Norbert Kiss ◽  
Judit Noll ◽  
Krisztina Becker ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Diffuse Reflectance ◽  
Ex Vivo ◽  
Second Harmonic ◽  
Light Emitting Diode ◽  
Autofluorescence Imaging ◽  
Nonlinear Optical Microscopy ◽  
Keratinopathic Ichthyosis

Keratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two different optical imaging methods. We used a newly developed light emitting diode (LED) based device to analyze autofluorescence signal at 405 nm excitation and diffuse reflectance at 526 nm in vivo. Mean autofluorescence intensity of the hyperkeratotic palmar skin was markedly higher in comparison to the healthy control (162.35 vs. 51.14). To further assess the skin status, we examined samples from affected skin areas ex vivo by nonlinear optical microscopy. Two-photon excited fluorescence and second-harmonic generation can visualize epidermal keratin and dermal collagen, respectively. We were able to visualize the structure of the epidermis and other skin changes caused by abnormal keratin formation. Taken together, we were able to show that such imaging modalities are useful for the diagnosis and follow-up of keratinopathic diseases.

Near-infrared autofluorescence imaging of cutaneous melanins and human skin in vivo

2009 ◽  
Vol 14 (2) ◽  
pp. 024017 ◽  
Author(s):  
Xiao Han ◽  
Harvey Lui ◽  
David I. McLean ◽  
Haishan Zeng
Keyword(s):  
Human Skin ◽  
Near Infrared ◽  
Autofluorescence Imaging

Phosphorescence, near-infrared absorption and nonlinear optical property of a new chiral organic crystal

2014 ◽  
Vol 07 (02) ◽  
pp. 1450011 ◽  
Author(s):  
Bei Zhang ◽  
Yu-Mei Zhao ◽  
Guo-Ping Yong
Keyword(s):  
Nonlinear Optical ◽  
Near Infrared ◽  
Nonlinear Optical Property ◽  
Second Harmonic ◽  
Single Step ◽  
Chiral Structure ◽  
Enantiomerically Pure ◽  
Chiral Induction ◽  
Multifunctional Material ◽  
Nir Absorption

A new enantiomerically pure compound was synthesized by the single step reduced reaction from 2-(imidazo[1,2-a]pyridin-2-yl)-2-oxo- N -(pyridin-2-yl)acetamide via chiral induction with D-tartaric acid in good yield. Single crystal data confirm this compound crystallizes in chiral space group P21. Transmission spectrum reveals that the crystal has low UV cut-off of 372 nm and has a good transmittance in the entire visible and near-infrared (NIR)region to 1100 nm, indicating its optical application. Kurtz powder test shows a good second harmonic generation (SHG) which also demonstrates its chiral structure. Moreover, this material exhibits blue phosphorescence with quantum yield of 3.6% and unusually NIR absorption between 1500 nm and 2500 nm. Therefore, this new chiral crystal is a promising multifunctional material for the blue phosphorescence, NIR absorption and nonlinear optical (NLO) applications.

In vivo imaging of small animals with optical tomography and near-infrared fluorescent probes

2002 ◽  
Author(s):  
Matthew R. Palmer ◽  
Yasushi Shibata ◽  
Jonathan B. Kruskal ◽  
Robert E. Lenkinski
Keyword(s):  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Optical Tomography ◽  
Small Animals

In vivo trans-rectal ultrasound coupled trans-rectal near-infrared optical tomography of canine prostate bearing transmissible venereal tumor

2009 ◽  
Author(s):  
Zhen Jiang ◽  
G. Reed Holyoak ◽  
Kenneth E. Bartels ◽  
Jerry W. Ritchey ◽  
Guan Xu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Optical Tomography ◽  
Rectal Ultrasound

scholarly journals In vivo near-infrared autofluorescence imaging of retinal pigment epithelial cells with 757 nm excitation

2018 ◽  
Vol 9 (12) ◽  
pp. 5946 ◽  
Author(s):  
Kate Grieve ◽  
Elena Gofas-Salas ◽  
R. Daniel Ferguson ◽  
José Alain Sahel ◽  
Michel Paques ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Near Infrared ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Autofluorescence Imaging ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

scholarly journals In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes

2018 ◽  
Author(s):  
Sez-Jade Chen ◽  
Nattawut Sinsuebphon ◽  
Alena Rudkouskaya ◽  
Margarida Barroso ◽  
Xavier Intes ◽  
...  
Keyword(s):  
Near Infrared ◽  
Specific Binding ◽  
Optical Tomography ◽  
Dynamic Imaging ◽  
Model Systems ◽  
Computationally Efficient ◽  
Time Resolved ◽  
Phasor Analysis

1AbstractWe introduce a simple new approach for time-resolved multiplexed analysis of complex systems using near-infrared (NIR) dyes, applicable to in vitro and in vivo studies. We first show that fast and precise in vitro quantification of NIR fluorophores lifetime and stoichiometry can be done using phasor analysis, a computationally efficient and user-friendly representation of complex fluorescence intensity decays obtained with pulsed laser excitation. We apply this approach to the study of binding equilibria by Förster resonant energy transfer (FRET), using two different model systems: primary/secondary antibody binding in vitro and ligand/receptor binding in cell cultures. We then extend our demonstration to dynamic imaging of the pharmacokinetics of transferrin binding to the transferrin receptor in live mice, elucidating the kinetic of differential transferrin accumulation in specific organs, straightforwardly differentiating specific from non-specific binding. Our method, implemented in a freely-available software package, has all the advantages of time-resolved NIR imaging, including better tissue penetration and background-free imaging, but simplifies and considerably speeds up data processing and interpretation, while remaining quantitative. These advances make this method attractive and of broad applicability for in vitro and in vivo molecular imaging, and could be extended to applications as diverse as image guided-surgery or optical tomography.

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

APPLICATIONS OF CHAMBER DEVICES WITH NONLINEAR OPTICAL IMAGE SYSTEM

2009 ◽  
Vol 21 (02) ◽  
pp. 123-129 ◽  
Author(s):  
Te-Hsuen Chen ◽  
Yi-You Huang
Keyword(s):  
High Speed ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Animal Experiments ◽  
Dorsal Skinfold Chamber ◽  
Optical Effects ◽  
Chamber Model ◽  
Novel Method ◽  
Stress And Deformation

In this study, we use laser to fabricate the plastic chamber for animal experiments in vivo. Laser cutting plastics process offers to cut a complex shape at high speed with the absence of hot stress and deformation. We combine this novel method to fabricate the dorsal skinfold plastic chamber and the ear plastic chamber. The chamber was applied on the nude mice and combined with optical system to improve the time of observation and the position of accuracy. The plastic dorsal skinfold chamber (DSC) exhibited good properties than the metallic DSC. The metallic DSC only offered monitoring for more than seven days after implantation. In contrast with the metallic DSC, the plastic DSC enabled monitoring to be observed for more than 12 days after implantation. This plastic DSC and ear chamber are more applications on short-term and long-term mode of disease. These chamber techniques combined with nonlinear microscopy will have many applications in clinic. The nonlinear optical effects have advantages for monitoring the biological processes, such as cellular autofluorescences were excited by two photons excitation and collagen structures were observed by second harmonic generation. The chamber model combines with nonlinear optical microscopy to visualizing cells and tissue structures in vivo. This chamber model allows repeat observation of the same sites for long periods of time and investigation of cells and collagen structures interaction without histological staining and sacrificed animals.

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