Larger depth of field for optical imaging methods

2007 ◽  
Author(s):  
J. J. Wang ◽  
S. G. Sang ◽  
Z. Wang ◽  
X. T. Mo ◽  
W. Y. Liu
Keyword(s):  
Optical Imaging ◽  
Depth Of Field ◽  
Imaging Methods

scholarly journals Assessment of Endothelin-A Receptor Expression in Subcutaneous and Orthotopic Thyroid Carcinoma Xenografts in Vivo Employing Optical Imaging Methods

Endocrinology ◽  
2012 ◽  
Vol 153 (6) ◽  
pp. 2907-2918 ◽  
Author(s):  
Katrin Büther ◽  
Matthijs G. Compeer ◽  
Jo G. R. De Mey ◽  
Otmar Schober ◽  
Michael Schäfers ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Optical Imaging ◽  
Near Infrared ◽  
Functional Performance ◽  
Thyroid Tumor ◽  
Receptor Expression ◽  
Imaging Methods ◽  
Carcinoma Cell Lines ◽  
Isolated Artery

Endothelin (ET) receptor dysregulation has been described in a number of pathophysiological processes, including cardiovascular disorders, renal failure, and cancer. The aim of this study was to evaluate the expression of the ET-A receptor (ETAR) in murine models of thyroid carcinoma using optical imaging methods. A recently developed near-infrared fluorescent tracer was first assessed in isolated artery preparations for its functional performance in comparison with known ETAR antagonists BQ123 and PD156707. Before evaluation of the tracer in vivo, different thyroid carcinoma cell lines were characterized with respect to their ET receptor expression by RT-PCR and autoradiography. In vivo, sc and orthotopic papillary thyroid tumor xenografts were clearly visualized by fluorescence reflectance imaging and fluorescence-mediated tomography up to 48 h after injection of the tracer. Binding specificity of the probe was demonstrated by predosing with PD156707 as a competing inhibitor. In conclusion, optical imaging with a fluorescent ETAR tracer allows the noninvasive imaging of tumor-associated ETAR expression in vivo. In the future, this technique may help surgeons to evaluate lesion dimensions in intraoperative settings (e.g. thyroidectomy).

Depth-of-field extension in optical imaging for rapid crystal screening

2021 ◽  
Vol 77 (4) ◽  
pp. 463-470
Author(s):  
Chen Li ◽  
Changqin Ding ◽  
Minghe Li ◽  
Jiayue Rong ◽  
Hilary Florian ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Wavelength Dependence ◽  
Optical Microscope ◽  
Field Extension ◽  
Protein Crystal ◽  
Depth Of Field ◽  
Theoretical Predictions ◽  
Second Harmonic Generation Imaging

The depth of field (DoF) was extended 2.8-fold to achieve rapid crystal screening by retrofitting a custom-designed micro-retarder array (µRA) in the optical beam path of a nonlinear optical microscope. The merits of the proposed strategy for DoF enhancement were assessed in applications of second-harmonic generation imaging of protein crystals. It was found that DoF extension increased the number of crystals detected while simultaneously reducing the number of `z-slices' required for screening. Experimental measurements of the wavelength-dependence of the extended DoF were in excellent agreement with theoretical predictions. These results provide a simple and broadly applicable approach to increase the throughput of existing nonlinear optical imaging methods for protein crystal screening.

Cellular Detection of G‐Quadruplexes by Optical Imaging Methods

2017 ◽  
Vol 76 (1) ◽  
Author(s):  
Souheila Amor ◽  
Sunny Y. Yang ◽  
Judy M. Y. Wong ◽  
David Monchaud
Keyword(s):  
Optical Imaging ◽  
Imaging Methods

scholarly journals Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method

2019 ◽  
Vol 12 (04) ◽  
pp. 1930005 ◽  
Author(s):  
Hexiang He ◽  
Xiangsheng Xie ◽  
Yikun Liu ◽  
Haowen Liang ◽  
Jianying Zhou
Keyword(s):  
Optical Imaging ◽  
Point Spread Function ◽  
Optical Memory ◽  
Ease Of Use ◽  
Large Field ◽  
Depth Of Field ◽  
Deconvolution Method ◽  
Scattering Medium ◽  
Point Spread ◽  
Spread Function

Visual perception of humans penetrating turbid medium is hampered by scattering. Various techniques have been prompted recently to recover optical imaging through turbid materials. Among them, speckle correlation based on deconvolution is one of the most attractive methods taking advantage of high imaging quality, robustness, ease-of-use, and ease-of-integration. By exploiting the point spread function (PSF) of the scattering system, large Field-of-View, extended Depth-of-Field, noninvasiveness and spectral resoluation are now available as successful solutions for high quality and multifunctional image reconstruction. In this paper, we review the progress of imaging through a scattering medium based on deconvolution method, including the principle, the breakthrough of the limitation of the optical memory effect, the improvement of the deconvolution algorithm and innovative applications.

Agreement among 3 Optical Imaging Methods for the Assessment of Optic Disc Topography

Ophthalmology ◽  
2005 ◽  
Vol 112 (12) ◽  
pp. 2149-2156 ◽  
Author(s):  
Esther M. Hoffmann ◽  
Christopher Bowd ◽  
Felipe A. Medeiros ◽  
Catherine Boden ◽  
Franz H. Grus ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Optic Disc ◽  
Imaging Methods

Optical imaging methods in medicine: how can we escape the plausibility trap?

2018 ◽  
Vol 63 (5) ◽  
pp. 507-510
Author(s):  
Werner Nahm ◽  
Christoph Hornberger ◽  
Ute Morgenstern ◽  
Stephan B. Sobottka
Keyword(s):  
Optical Imaging ◽  
Imaging Methods
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