Optical imaging methods for label free detection of microplastics in cells, tissues and environmental organisms (Conference Presentation)

2020 ◽  
Author(s):  
Alvaro Barroso Pena ◽  
Steffi Ketelhut ◽  
Matthias Godejohann ◽  
Björn Kemper ◽  
Jürgen Schnekenburger
Keyword(s):  
Optical Imaging ◽  
Label Free ◽  
Imaging Methods ◽  
Label Free Detection ◽  
In Cells

Biomimetic Nanochannel-Ionchannel Hybrid for Ultrasensitive and Label-Free Detection of MicroRNA in Cells

2019 ◽  
Vol 91 (5) ◽  
pp. 3582-3589 ◽  
Author(s):  
Xiao-Ping Zhao ◽  
Fei-Fei Liu ◽  
Wen-Chao Hu ◽  
Muhammad Rizwan Younis ◽  
Chen Wang ◽  
...  
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
In Cells

scholarly journals Biocompatible Nanoparticles: High-Resolution Label-Free Detection of Biocompatible Polymeric Nanoparticles in Cells (Part. Part. Syst. Charact. 3/2018)

2018 ◽  
Vol 35 (3) ◽  
pp. 1870008
Author(s):  
Elisabetta Pancani ◽  
Jérémie Mathurin ◽  
Sylvie Bilent ◽  
Marie-Françoise Bernet-Camard ◽  
Alexandre Dazzi ◽  
...  
Keyword(s):  
High Resolution ◽  
Polymeric Nanoparticles ◽  
Label Free ◽  
Biocompatible Nanoparticles ◽  
Label Free Detection ◽  
In Cells

scholarly journals High-Resolution Label-Free Detection of Biocompatible Polymeric Nanoparticles in Cells

2018 ◽  
Vol 35 (3) ◽  
pp. 1700457 ◽  
Author(s):  
Elisabetta Pancani ◽  
Jérémie Mathurin ◽  
Sylvie Bilent ◽  
Marie-Françoise Bernet-Camard ◽  
Alexandre Dazzi ◽  
...  
Keyword(s):  
High Resolution ◽  
Polymeric Nanoparticles ◽  
Label Free ◽  
Label Free Detection ◽  
In Cells

scholarly journals Label-Free Detection of Mitochondrial Distribution in Cells by Nonresonant Raman Microspectroscopy

2007 ◽  
Vol 93 (2) ◽  
pp. 668-673 ◽  
Author(s):  
Christian Matthäus ◽  
Tatyana Chernenko ◽  
Judith A. Newmark ◽  
Carol M. Warner ◽  
Max Diem
Keyword(s):  
Raman Microspectroscopy ◽  
Label Free ◽  
Label Free Detection ◽  
Mitochondrial Distribution ◽  
In Cells

Nanoscale optical imaging in chemistry

2020 ◽  
Vol 49 (16) ◽  
pp. 6087-6112 ◽  
Author(s):  
Andrew J. Wilson ◽  
Dinumol Devasia ◽  
Prashant K. Jain
Keyword(s):  
Optical Imaging ◽  
Surface Science ◽  
Single Molecule ◽  
Chemical Processes ◽  
Label Free ◽  
Imaging Methods ◽  
Wide Range

New advances in label-free optical imaging methods are allowing a wide range of chemical processes in surface science, catalysis, and photochemistry to be probed on the nanoscale and single-molecule levels.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

scholarly journals Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1872
Author(s):  
Holger Schulze ◽  
Harry Wilson ◽  
Ines Cara ◽  
Steven Carter ◽  
Edward N. Dyson ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Electrochemical Impedance ◽  
Point Of Care ◽  
Branched Polymers ◽  
Label Free ◽  
Conformation Change ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Staphylococcus Carnosus ◽  
Label Free Detection

Rapid point of care tests for bacterial infection diagnosis are of great importance to reduce the misuse of antibiotics and burden of antimicrobial resistance. Here, we have successfully combined a new class of non-biological binder molecules with electrochemical impedance spectroscopy (EIS)-based sensor detection for direct, label-free detection of Gram-positive bacteria making use of the specific coil-to-globule conformation change of the vancomycin-modified highly branched polymers immobilized on the surface of gold screen-printed electrodes upon binding to Gram-positive bacteria. Staphylococcus carnosus was detected after just 20 min incubation of the sample solution with the polymer-functionalized electrodes. The polymer conformation change was quantified with two simple 1 min EIS tests before and after incubation with the sample. Tests revealed a concentration dependent signal change within an OD600 range of Staphylococcus carnosus from 0.002 to 0.1 and a clear discrimination between Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. This exhibits a clear advancement in terms of simplified test complexity compared to existing bacteria detection tests. In addition, the polymer-functionalized electrodes showed good storage and operational stability.

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