Surface functionalization of a microfluidic biosensor for bacteria detection and identification

2007 ◽  
Author(s):  
Douglas A. Boehm ◽  
Philip Gottlieb ◽  
Susan Z. Hua
Keyword(s):  
Surface Functionalization ◽  
Bacteria Detection ◽  
Detection And Identification ◽  
Microfluidic Biosensor

Modern methods of bacteria detection and identification

2013 ◽  
Vol 4 (1) ◽  
pp. 65-72
Author(s):  
Dorota Żabicka ◽  
Elżbieta Literacka
Keyword(s):  
Bacteria Detection ◽  
Detection And Identification ◽  
Modern Methods

scholarly journals Polarization Induced Electro-Functionalization of Pore Walls: A Contactless Technology

Biosensors ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 121 ◽  
Author(s):  
Bouchet-Spinelli ◽  
Descamps ◽  
Liu ◽  
Ismail ◽  
Pham ◽  
...  
Keyword(s):  
Solid State ◽  
Surface Functionalization ◽  
Silicon Oxide ◽  
Pore Wall ◽  
Electrical Current ◽  
Microfluidic Channels ◽  
The Core ◽  
Detection And Identification ◽  
Oxide Membrane ◽  
Silicon Silicon

This review summarizes recent advances in micro- and nanopore technologies with a focus on the functionalization of pores using a promising method named contactless electro-functionalization (CLEF). CLEF enables the localized grafting of electroactive entities onto the inner wall of a micro- or nano-sized pore in a solid-state silicon/silicon oxide membrane. A voltage or electrical current applied across the pore induces the surface functionalization by electroactive entities exclusively on the inside pore wall, which is a significant improvement over existing methods. CLEF’s mechanism is based on the polarization of a sandwich-like silicon/silicon oxide membrane, creating electronic pathways between the core silicon and the electrolyte. Correlation between numerical simulations and experiments have validated this hypothesis. CLEF-induced micro- and nanopores functionalized with antibodies or oligonucleotides were successfully used for the detection and identification of cells and are promising sensitive biosensors. This technology could soon be successfully applied to planar configurations of pores, such as restrictions in microfluidic channels.

On-chip microfluidic biosensor for bacterial detection and identification

2007 ◽  
Vol 126 (2) ◽  
pp. 508-514 ◽  
Author(s):  
Douglas A. Boehm ◽  
Philip A. Gottlieb ◽  
Susan Z. Hua
Keyword(s):  
Bacterial Detection ◽  
Detection And Identification ◽  
Microfluidic Biosensor ◽  
On Chip

Polydiacetylene-Based Liposomes: An "Optical Tongue" for Bacteria Detection and Identification

2009 ◽  
Vol 86 (3) ◽  
pp. 373 ◽  
Author(s):  
Matthew R. West ◽  
Timothy W. Hanks ◽  
Rhett T. Watson
Keyword(s):  
Bacteria Detection ◽  
Detection And Identification

In Vivo Binding of the Salivary Glycoprotein EP-GP (Identical to GCDFP-15) to Oral and Non-Oral Bacteria Detection and Identification of EP-GP Binding Species

1997 ◽  
Vol 378 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Léon C. P. M. Schenkels ◽  
Els Walgreen-Weterings ◽  
Lauran C. J. M. Oomen ◽  
Jan G. M. Bolscher ◽  
Enno C. I. Veerman ◽  
...  
Keyword(s):  
Oral Bacteria ◽  
Bacteria Detection ◽  
In Vivo Binding ◽  
Detection And Identification

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

115: Detection and Identification of Bacterial DNA and HIV Gag in the Semen of HIV Infected Men

2004 ◽  
Vol 171 (4S) ◽  
pp. 30-30
Author(s):  
Robert C. Eyre ◽  
Ann A. Kiessling ◽  
Thomas E. Mullen ◽  
Rachel L. Kiessling
Keyword(s):  
Bacterial Dna ◽  
Detection And Identification
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