Wide-field two-photon microscopy: features and advantages for biomedical applications

2007 ◽  
Author(s):  
S. Wachsmann-Hogiu ◽  
J. Y. Hwang ◽  
E. Lindsley ◽  
D. L. Farkas
Keyword(s):  
Biomedical Applications ◽  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon

Encrypted wide-field two-photon microscopy with single-pixel detection and compressed sensing

2020 ◽  
Vol 13 (3) ◽  
pp. 032007
Author(s):  
Yu-Xuan Ren ◽  
Cihang Kong ◽  
Hongsen He ◽  
Xinglin Zeng ◽  
Kevin K. Tsia ◽  
...  
Keyword(s):  
Compressed Sensing ◽  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Single Pixel

scholarly journals Lanthanide nano-drums: a new class of molecular nanoparticles for potential biomedical applications

2014 ◽  
Vol 175 ◽  
pp. 241-255 ◽  
Author(s):  
Richard A. Jones ◽  
Annie J. Gnanam ◽  
Jonathan F. Arambula ◽  
Jessica N. Jones ◽  
Jagannath Swaminathan ◽  
...  
Keyword(s):  
Total Internal Reflection ◽  
Biomedical Applications ◽  
Near Infrared ◽  
Photophysical Properties ◽  
Diagnostic Systems ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Self Assembling ◽  
Molecular Nanoparticles

We are developing a new class of lanthanide-based self-assembling molecular nanoparticles as potential reporter molecules for imaging, and as multi-functional nanoprobes or nanosensors in diagnostic systems. These lanthanide “nano-drums” are homogeneous 4d–4f clusters approximately 25 to 30 Å in diameter that can emit from the visible to near-infrared (NIR) wavelengths. Here, we present syntheses, crystal structures, photophysical properties, and comparative cytotoxicity data for six nano-drums containing either Eu, Tb, Lu, Er, Yb or Ho. Imaging capabilities of these nano-drums are demonstrated using epifluorescence, total internal reflection fluorescence (TIRF), and two-photon microscopy. We discuss how these molecular nanoparticles can to be adapted for a range of assays, particularly by taking advantage of functionalization strategies with chemical moieties to enable conjugation to protein or nucleic acids.

Upconverting nanoparticles: a versatile platform for wide-field two-photon microscopy and multi-modal in vivo imaging

2015 ◽  
Vol 44 (6) ◽  
pp. 1302-1317 ◽  
Author(s):  
Yong Il Park ◽  
Kang Taek Lee ◽  
Yung Doug Suh ◽  
Taeghwan Hyeon
Keyword(s):  
In Vivo Imaging ◽  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon

Upconverting nanoparticles (UCNPs) enable the establishment of a novel UCNP-based platform for wide-field two-photon microscopy and multimodal in vivo imaging.

scholarly journals Wide-field Two Photon Microscopy

2015 ◽  
Vol 10 (5) ◽  
pp. 39-42 ◽  
Author(s):  
Nadine Döge ◽  
Erik Thiel ◽  
Gunter Seewald ◽  
Stephanie Albrecht ◽  
Annika Vogt ◽  
...  
Keyword(s):  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon
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