Wide-field speckle imaging and two-photon microscopy for the investigation of cerebral blood flow in vivo in mice models of obesity

2018 ◽  
Author(s):  
Frédéric Pain ◽  
Hirac Gurden ◽  
Haleh Soleimanzad
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Wide Field ◽  
Speckle Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Two-photon microscopy with double-circle trajectories for in vivo cerebral blood flow measurements

2013 ◽  
Vol 54 (5) ◽  
Author(s):  
Andrin Landolt ◽  
Dominik Obrist ◽  
Matthias Wyss ◽  
Matthew Barrett ◽  
Dominik Langer ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Flow Measurements ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Blood Flow Measurements

Wide field speckle imaging for the investigation of cerebral blood flow in vivo in murine model of obesity

2019 ◽  
Author(s):  
Frédéric Pain ◽  
Haleh Soleimanzad ◽  
François Smekens ◽  
Christophe Magnan ◽  
Hirac Gurden
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Murine Model ◽  
Wide Field ◽  
Speckle Imaging

Upconverting nanoparticles: a versatile platform for wide-field two-photon microscopy and multi-modal in vivo imaging

2015 ◽  
Vol 44 (6) ◽  
pp. 1302-1317 ◽  
Author(s):  
Yong Il Park ◽  
Kang Taek Lee ◽  
Yung Doug Suh ◽  
Taeghwan Hyeon
Keyword(s):  
In Vivo Imaging ◽  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon

Upconverting nanoparticles (UCNPs) enable the establishment of a novel UCNP-based platform for wide-field two-photon microscopy and multimodal in vivo imaging.

Effect of caffeine on theophyliine on cerebral blood flow response to brain activation: In vitro and in vivo studies

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S198-S198
Author(s):  
Joseph R Meno ◽  
Thien-son K Nguyen ◽  
Elise M Jensen ◽  
G Alexander West ◽  
Leonid Groysman ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Brain Activation ◽  
In Vivo Studies ◽  
Blood Flow Response ◽  
Flow Response

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

Sensory Transmission is Bi-directionally Modulated by Astrocytic Ca2+ in Barrel Cortex of Behaving Mice

2021 ◽  
Author(s):  
Simeng Gu ◽  
Wei Wang ◽  
Kuan Zhang ◽  
Rou Feng ◽  
Naling Li ◽  
...  
Keyword(s):  
Sensory Input ◽  
Barrel Cortex ◽  
Synaptic Function ◽  
Metabolic Clearance ◽  
Sleep State ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Sensory Transmission

Abstract Different effects of astrocyte during sleep and awake have been extensively studied, especially for metabolic clearance by the glymphatic system, which works during sleep and stops working during waking states. However, how astrocytes contribute to modulation of sensory transmission during sleep and awake animals remain largely unknown. Recent advances in genetically encoded Ca2+ indicators have provided a wealth of information on astrocytic Ca2+, especially in their fine perisynaptic processes, where astrocytic Ca2+ most likely affects the synaptic function. Here we use two-photon microscopy to image astrocytic Ca2+ signaling in freely moving mice trained to run on a wheel in combination with in vivo whole-cell recordings to evaluate the role of astrocytic Ca2+ signaling in different behavior states. We found that there are two kinds of astrocytic Ca2+ signaling: a small long-lasting Ca2+ increase during sleep state and a sharp widespread but short-long-lasting Ca2+ spike when the animal was awake (fluorescence increases were 23.2 ± 14.4% for whisker stimulation at sleep state, compared with 73.3 ± 11.7% for at awake state, paired t-test, p < 0.01). The small Ca2+ transients decreased extracellular K+, hyperpolarized the neurons, and suppressed sensory transmission; while the large Ca2+ wave enhanced sensory input, contributing to reliable sensory transmission in aroused states. Locus coeruleus activation works as a switch between these two kinds of astrocytic Ca2+ elevation. Thus, we show that cortical astrocytes play an important role in processing of sensory input. These two types of events appear to have different pharmacological sources and may play a different role in facilitating the efficacy of sensory transmission.

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