Laser diffractometry of erythrocyte deformation under hypo-osmotic hemolysis

2000 ◽  
Author(s):  
Stanislav S. Bessmeltsev ◽  
Alexander V. Lendiaev ◽  
Yulia A. Skvortsova ◽  
Vladimir A. Tarlykov
Keyword(s):  
Osmotic Hemolysis ◽  
Laser Diffractometry ◽  
Erythrocyte Deformation

Osmotic hemolysis of nucleated erythrocytes

1964 ◽  
Vol 35 (2) ◽  
pp. 230-238 ◽  
Author(s):  
Y.F. Frei ◽  
K. Perk
Keyword(s):  
Osmotic Hemolysis

Function of glycerol as a protectant against osmotic hemolysis of human erythrocytes

Cryobiology ◽  
1989 ◽  
Vol 26 (6) ◽  
pp. 579
Author(s):  
D.Y. Gag ◽  
J.A. Kornblatt ◽  
S. Lin
Keyword(s):  
Human Erythrocytes ◽  
Osmotic Hemolysis

scholarly journals Tenuazonic acid: a promising antitubercular principle from Alternaria alternate

2011 ◽  
Vol 2 (1) ◽  
pp. 17 ◽  
Author(s):  
Visalakchi Sonaimuthu ◽  
Swati Parihar ◽  
Jay Prakash Thakur ◽  
Suaib Luqman ◽  
Dharmendra Saikia ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Alternaria Alternata ◽  
Active Metabolite ◽  
Antitubercular Activity ◽  
Model System ◽  
Human Erythrocytes ◽  
Tenuazonic Acid ◽  
Fungal Metabolite ◽  
Osmotic Hemolysis ◽  
Alternaria Alternate

Bioactivity guided isolation of dichloromethane extract of <em>Alternaria alternata</em> identified tenuazonic acid (1) as potentially active against <em>Mycobacterium tuberculosis</em> H37Rv, MIC at 250 μg/mL concentration. This active metabolite 1, was also evaluated for osmotic hemolysis using the erythrocyte as a model system. It was observed that this fungal metabolite showing antitubercular activity exhibited concentration dependent toxicity to human erythrocytes.

scholarly journals Erythrocyte deformation in shear flow: influences of internal viscosity, membrane stiffness, and hematocrit

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 727-734 ◽  
Author(s):  
K Kon ◽  
N Maeda ◽  
T Shiga
Keyword(s):  
Viscoelastic Properties ◽  
Shear Force ◽  
Flow Lines ◽  
Extracellular Medium ◽  
Intact Cells ◽  
Suspension Viscosity ◽  
Applied Shear Stress ◽  
Erythrocyte Deformation ◽  
Membrane Stiffness ◽  
Internal Viscosity

The effect of shear force (depending on shear rate and viscosity of extracellular medium) and hematocrit of RBC suspension on RBC deformation was studied quantitatively using a cone-plate rheoscope with various kinds of cells, ie, partially hemolyzed (PH) cells, density-fractionated intact cells, and diamide-treated cells. The deformation index (DI) of ellipsoidally deformed cells was shown to be a function of beta gamma eta ex(eta ex/eta in)alpha, where gamma eta ex is applied shear stress, eta ex and eta in are external and internal viscosities, respectively, and alpha and beta are adjustable parameters related to the membrane viscoelastic properties. The increase of suspension viscosity at higher hematocrits (Hts) generally enhanced the ellipsoidal deformation of cells, in the same manner as increasing the suspending medium viscosity of a diluted cell suspension. The suppressing effect on cell deformation appeared above a certain Ht. When intact cells were mixed with glutaraldehyde-treated, hardened cells, the ellipsoidal deformation of intact cells was disturbed. The suppression of deformation probably occurred through disturbance of laminar flow-lines around intact cells.

The effect of albumin on osmotic hemolysis

1960 ◽  
Vol 20 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Leah Miriam Lowenstein
Keyword(s):  
Osmotic Hemolysis

Participation of cAMP in a signal-transduction pathway relating erythrocyte deformation to ATP release

2001 ◽  
Vol 281 (4) ◽  
pp. C1158-C1164 ◽  
Author(s):  
Randy S. Sprague ◽  
Mary L. Ellsworth ◽  
Alan H. Stephenson ◽  
Andrew J. Lonigro
Keyword(s):  
Signal Transduction ◽  
Adenylyl Cyclase ◽  
Atp Release ◽  
Signal Transduction Pathway ◽  
Intracellular Camp ◽  
Transmembrane Conductance Regulator ◽  
Transduction Pathway ◽  
Camp Analog ◽  
Dependent Protein ◽  
Erythrocyte Deformation

Previously, we reported that red blood cells (RBCs) of rabbits and humans release ATP in response to mechanical deformation and that this release of ATP requires the activity of the cystic fibrosis transmembrane conductance regulator (CFTR). It was reported that cAMP, acting through a cAMP-dependent protein kinase, PKA, is an activator of CFTR. Here we investigate the hypothesis that cAMP stimulates ATP release from RBCs. Incubation of human and rabbit RBCs with the direct activator of adenylyl cyclase, forskolin (10 or 100 μM), with IBMX (100 μM), resulted in ATP release and increases in intracellular cAMP. In addition, epinephrine (1 μM), a receptor-mediated activator of adenylyl cyclase, stimulated ATP release from rabbit RBCs. Moreover, incubation of human and rabbit RBCs with an active cAMP analog [adenosine 3′5′-cyclic monophosphorothioate Sp-isomer (Sp-cAMP, 100 μM)] resulted in ATP release. In contrast, forskolin and Sp-cAMP were without effect on dog RBCs, cells known not to release ATP in response to deformation. When rabbit RBCs were incubated with the inactive cAMP analog and inhibitor of PKA activity, adenosine 3′,5′-cyclic monophosphorothioate Rp-isomer (100 μM), deformation-induced ATP release was attenuated. These results are consistent with the hypothesis that adenylyl cyclase and cAMP are components of a signal-transduction pathway relating RBC deformation to ATP release from human and rabbit RBCs.

Erythrocyte Deformation in Human Muscular Dystrophy

Science ◽  
1974 ◽  
Vol 184 (4133) ◽  
pp. 165-166 ◽  
Author(s):  
D. W. Matheson ◽  
J. L. Howland
Keyword(s):  
Muscular Dystrophy ◽  
Erythrocyte Deformation

Role of Organic Matter and Carbonates in Soil Aggregation Estimated Using Laser Diffractometry

Pedosphere ◽  
2011 ◽  
Vol 21 (5) ◽  
pp. 566-572 ◽  
Author(s):  
I. VIRTO ◽  
N. GARTZIA-BENGOETXEA ◽  
O. FERNÁNDEZ-UGALDE
Keyword(s):  
Organic Matter ◽  
Soil Aggregation ◽  
Laser Diffractometry
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