Recovery of intrinsic fluorescence of tissue mimicking model media and human breast tissues from spatially resolved fluorescence and simultaneous evaluation of optical transport parameters

2006 ◽  
Author(s):  
V.L.N. Sridhar Raja ◽  
Sharad Gupta ◽  
Asima Pradhan
Keyword(s):  
Intrinsic Fluorescence ◽  
Human Breast ◽  
Transport Parameters ◽  
Spatially Resolved ◽  
Simultaneous Evaluation ◽  
Optical Transport ◽  
Breast Tissues

scholarly journals P01.06 Spatially-resolved, highly multiplexed biomarker analysis of cancerous and normal human breast tissues

2020 ◽  
Vol 8 (Suppl 2) ◽  
pp. A10.2-A11
Author(s):  
O Braubach ◽  
S Basak ◽  
M Gallina ◽  
W Lee ◽  
J Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Microenvironment ◽  
Human Breast Cancer ◽  
Cell Types ◽  
Normal Breast ◽  
Human Breast ◽  
Spatially Resolved ◽  
Biomarker Analysis ◽  
Part Time ◽  
Breast Tissues

BackgroundBreast cancer has a high incidence rate and there is a need to develop new diagnostic tools and treatment regimens. Progress has, unfortunately, been slow and new technologies are urgently needed to generate a comprehensive understanding of breast cancer biology. Highly multiplexed imaging is an emerging tool that can help to unravel the complexities of the tumor microenvironment. This technology enables the detection of tens of biomarkers within a tissue specimen, and allows comprehensive cell phenotyping, biomarker quantification and spatial localization at cellular resolution. Such measurements can, in turn, provide insights into disease mechanisms and identify potential treatment targets. We demonstrate the development of a breast cancer specific CO-Detection by indEXing (CODEX®) panel that allows simultaneous in situ imaging of more than 30+ antibody markers.Materials and MethodsCODEX® relies on a DNA-based tagging approach, whereby antibodies are labeled with specific oligonucleotide tags (barcodes) and dye-oligonucleotides (reporters) are iteratively hybridized and dehybridized across multiple cycles. This process is completely automated through the CODEX® instrument and readily deployable on commercially available fluorescence microscopy systems. Using a 30+ antibody CODEX® panel, we compared formalin-fixed paraffin embedded (FFPE) human breast cancer tissues at different stages of disease progression with normal breast tissues. Our antibody panel was designed to detect cancer cells as well as non-malignant cells in order to comprehensively survey the tumor microenvironment and normal control tissues. Data were analyzed using the CODEX® software suite to identify key cell types and analyze spatial associations.ResultsOur analyses revealed more than 20 distinct cell types in human breast cancer and normal tissues. Cell populations, biomarker expression and cellular spatial distributions differed distinctly between cancerous and normal breast tissues. Differences were robust, repeatedly observed and indicative of altered cellular milieus in normal versus cancerous breast tissues.ConclusionsCollectively, these data establish CODEX® as a readily deployable and practical tool for spatially-resolved, highly multiplexed biomarker analysis of human FFPE samples.Disclosure InformationO. Braubach: A. Employment (full or part-time); Significant; Akoya Biosciences. S. Basak: A. Employment (full or part-time); Significant; Akoya Biosciences. M. Gallina: A. Employment (full or part-time); Significant; Akoya Biosciences. W. Lee: A. Employment (full or part-time); Significant; Akoya Biosciences. J. Kim: A. Employment (full or part-time); Significant; Akoya Biosciences. C. Hempel: A. Employment (full or part-time); Significant; Akoya Biosciences. E. Williams: A. Employment (full or part-time); Significant; Akoya Biosciences. O. Shang: A. Employment (full or part-time); Significant; Akoya Biosciences. B. Cheung: A. Employment (full or part-time); Significant; Akoya Biosciences. J. Kennedy-Darling: A. Employment (full or part-time); Significant; Akoya Biosciences.

scholarly journals Oncologic photodynamic diagnosis and therapy: confocal Raman/fluorescence imaging of metal phthalocyanines in human breast cancer tissue in vitro

The Analyst ◽  
2014 ◽  
Vol 139 (21) ◽  
pp. 5547-5559 ◽  
Author(s):  
Halina Abramczyk ◽  
Beata Brozek-Pluska ◽  
Jakub Surmacki ◽  
Jacek Musial ◽  
Radzislaw Kordek
Keyword(s):  
Breast Cancer Tissue ◽  
Photodynamic Diagnosis ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue ◽  
Metal Phthalocyanines ◽  
Confocal Fluorescence ◽  
Confocal Raman ◽  
Breast Tissues

Raman microspectroscopy and confocal Raman imaging combined with confocal fluorescence were used to study the distribution and aggregation of aluminum tetrasulfonated phthalocyanine (AlPcS4) in breast tissues.

scholarly journals AKT1 is differentially expressed in primary tumors and brain metastases in humans with breast cancer.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Breast Cancer ◽  
Brain Metastases ◽  
Metastatic Breast ◽  
Metastatic Tumor ◽  
Clinical Problem ◽  
Central Nervous System Metastasis ◽  
Human Breast ◽  
Primary Tumors ◽  
Breast Tissues ◽  
The Brain

Metastasis to the brain is a clinical problem in patients with breast cancer (1-3). We mined published microarray data (4, 5) to compare primary and metastatic tumor transcriptomes to discover genes associated with brain metastasis in patients with metastatic breast cancer. We report here the differential expression of the protein kinase AKT1 in the primary tumors and brain metastases of humans with breast cancer. AKT1 mRNA was present at significantly increased quantities in brain metastatic tissues as compared to primary tumors of the breast. These data combined suggest that up-regulation of AKT1 is a conserved event, both during transformation of breast tissues and progression to central nervous system metastasis and further point to potential importance of AKT1 modulation during progression of human breast cancer.

Fluorescence study of normal, benign, and malignant human breast tissues

2000 ◽  
Author(s):  
Asima Pradhan ◽  
R. N. Panda ◽  
Maya S. Nair ◽  
B. V. Laxmi ◽  
Asha Agarwal ◽  
...  
Keyword(s):  
Human Breast ◽  
Fluorescence Study ◽  
Breast Tissues

Differences in Polyamine Metabolism between Carcinomatous and Uninvolved Human Breast Tissues

1986 ◽  
Vol 1 (2) ◽  
pp. 77-80 ◽  
Author(s):  
Marta Romano ◽  
Maria Assunta Santacroce ◽  
Patrizia Bonelli ◽  
Luigi Cecco ◽  
Maria Cerra
Keyword(s):  
Cell Growth ◽  
Breast Tissue ◽  
Lower Activity ◽  
Polyamine Metabolism ◽  
Biosynthetic Enzymes ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Breast Tissues

The polyamine biosynthetic enzymes ODC and SAMDC show higher activity in carcinomatous human breast tissue than in uninvolved tissue of the same breast; the interconversion enzyme PAO shows significantly lower activity in carcinomatous than uninvolved tissue. The polyamine metabolism in carcinomatous human breast tissue thus appears to differ from that in uninvolved tissue. Intracellular polyamine concentrations, particularly spermine, are high in carcinomatous tissue. This increase and that of the biosynthetic enzymes suggest that a higher polyamine concentration is needed for carcinomatous cell growth. If this is the case, the lower PAO activity in carcinomatous tissue may be explained as a mechanism that conserves the high intracellular polyamine concentration.

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