Applications of dynamic light scattering, fluorescence microscopy and fluorescence spectroscopy in DB-67 liposomal formulation studies

2005 ◽  
Author(s):  
Stefan Kruszewski ◽  
Blanka Ziomkowska ◽  
Michał Cyrankiewicz ◽  
Lori Latus ◽  
David Bom
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Microscopy ◽  
Fluorescence Spectroscopy ◽  
Liposomal Formulation

Comparison of Optical Properties of Pyrazoline Derivative Nanoparticles

2007 ◽  
Vol 119 ◽  
pp. 39-42 ◽  
Author(s):  
Hyo Sim Kang ◽  
Sun Wha Oh ◽  
Young Soo Kang
Keyword(s):  
Optical Properties ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Spectroscopy ◽  
Phenyl Ring ◽  
Organic Nanoparticles ◽  
Size Dependent ◽  
Reprecipitation Method ◽  
Pyrazoline Derivative

We prepared various pyrazoline derivatives which possess dimethylamino-, ethoxy-, isopropyl-phenyl ring at the 5-position of pyrazoline. The nanoparticles of pyrazoline derivative ranging from tens to hundreds of nanometers by the reprecipitation method have been successfully prepared and their optical size-dependent properties have been investigated with UV-vis, fluorescence spectroscopy, DLS (Dynamic Light Scattering) and SEM. The size-dependent optical properties of pyrazoline organic nanoparticles have been observed in the order of dimethylamino- > ethoxy- > isopropyl- in electro-donating characters.

scholarly journals Glycodendrimers: tools to explore multivalent galectin-1 interactions

2015 ◽  
Vol 11 ◽  
pp. 739-747 ◽  
Author(s):  
Jonathan M Cousin ◽  
Mary J Cloninger
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Microscopy ◽  
Binding Sites ◽  
Carcinoma Cells ◽  
Large Excess ◽  
Human Prostate Carcinoma ◽  
Typical Function ◽  
Cellular Aggregation ◽  
Du145 Cells

Four generations of lactose-functionalized polyamidoamine (PAMAM) were employed to further the understanding of multivalent galectin-1 mediated interactions. Dynamic light scattering and fluorescence microscopy were used to study the multivalent interaction of galectin-1 with the glycodendrimers in solution, and glycodendrimers were observed to organize galectin-1 into nanoparticles. In the presence of a large excess of galectin-1, glycodendrimers nucleated galectin-1 into nanoparticles that were remarkably homologous in size (400–500 nm). To understand augmentation of oncologic cellular aggregation by galectin-1, glycodendrimers were used in cell-based assays with human prostate carcinoma cells (DU145). The results revealed that glycodendrimers provided competitive binding sites for galectin-1, which diverted galectin-1 from its typical function in cellular aggregation of DU145 cells.

scholarly journals Thermo-responsiveness of polysiloxanes grafted with poly(dimethyl acrylamide) segments

2012 ◽  
Vol 10 (4) ◽  
pp. 1338-1348 ◽  
Author(s):  
Anca Rusu Hodorog ◽  
Constanta Ibanescu ◽  
Ioana Moleavin ◽  
Nicolae Hurduc
Keyword(s):  
Phase Transition ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Spectroscopy ◽  
Methyl Methacrylate ◽  
Lower Critical Solution Temperature ◽  
Solution Temperature ◽  
Side Chain ◽  
Critical Solution Temperature ◽  
Aggregation Phenomena

AbstractThermo-sensitive polymers are of outstanding importance due to their ability to undergo controlled major changes in their properties as a response to minor modifications in temperature. The syntheses of novel polymers by grafting polysiloxane containing chlorobenzyl groups in the side chain by the homopolymerization of N,N′-dimethyl acrylamide (DMA) or the copolymerization of DMA and methyl methacrylate (MMA) using SET-LRP technique are presented. The polymers were characterized by 1H-NMR, UV, and fluorescence spectroscopy, and DSC. The thermo-sensitivity and the lower critical solution temperature (LCST), as well as the aggregation phenomena during the phase transition are evidenced by dynamic light scattering (DLS) and rheology.

scholarly journals A coumarin based gold(i)-alkynyl complex: a new class of supramolecular hydrogelators

2015 ◽  
Vol 13 (7) ◽  
pp. 2026-2033 ◽  
Author(s):  
Artur J. Moro ◽  
Bertrand Rome ◽  
Elisabet Aguiló ◽  
Julià Arcau ◽  
Rakesh Puttreddy ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Microscopy ◽  
New Class

A phosphine-gold(i)-alkynyl-coumarin complex, [Au{7-(prop-2-ine-1-yloxy)-1-benzopyran-2-one}(DAPTA)] (1), was synthesized and the formation of long luminescent fibers in solution was characterized via fluorescence microscopy and dynamic light scattering.

Effect of counterions on the binding affinity of Na+ ions with phospholipid membranes

RSC Advances ◽  
2016 ◽  
Vol 6 (87) ◽  
pp. 83916-83925 ◽  
Author(s):  
Pabitra Maity ◽  
Baishakhi Saha ◽  
Gopinatha Suresh Kumar ◽  
Sanat Karmakar
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Zeta Potential ◽  
Fluorescence Spectroscopy ◽  
Binding Affinity ◽  
Isothermal Titration Calorimetry ◽  
Titration Calorimetry ◽  
Phospholipid Membranes ◽  
Na Ions

We have systematically investigated the effect of counterions on the interaction of the Na+ ion with phospholipid membranes using dynamic light scattering, zeta potential, isothermal titration calorimetry and fluorescence spectroscopy techniques.

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