scholarly journals Interaction of the 18.5-kD isoform of myelin basic protein with Ca2+-calmodulin: Effects of deimination assessed by intrinsic Trp fluorescence spectroscopy, dynamic light scattering, and circular dichroism

2003 ◽  
Vol 12 (7) ◽  
pp. 1507-1521 ◽  
Author(s):  
David S. Libich ◽  
Christopher M.D. Hill ◽  
Ian R. Bates ◽  
F. Ross Hallett ◽  
Souzan Armstrong ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Fluorescence Spectroscopy ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Trp Fluorescence ◽  
Circular Dichroïsm

scholarly journals Biophysical characterization of 21.5-kDa myelin basic protein (MBP) and the effects of zinc on its structure

SURG Journal ◽  
2014 ◽  
Vol 7 (3) ◽  
pp. 30-41
Author(s):  
Danielle K. Lanthier ◽  
Kenrick A. Vassall ◽  
George Harauz
Keyword(s):  
Circular Dichroism ◽  
Fluorescence Spectroscopy ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Critical Role ◽  
Intrinsically Disordered Protein ◽  
Biophysical Characterization ◽  
Intrinsically Disordered ◽  
Circular Dichroïsm

Myelin Basic Protein (MBP) is a highly abundant protein in central nervous system (CNS) myelin that has a critical role in its proper formation and functioning. The 21.5-kDa isoform of MBP has been shown to be selectively imported into the nucleus of myelin-producing cells, oligodendrocytes, and may be involved in signaling pathways that affect the formation and recovery of CNS myelin. The first step in understanding potential nuclear binding partners of 21.5-kDa MBP is to characterize the structure of the protein. In this study, circular dichroism and fluorescence spectroscopy were used to analyze the structure of 21.5-kDa rmMBP (recombinant murine MBP) in vitro in the presence and absence of Zn2+, an abundant trace metal in CNS myelin that has been suggested to affect MBP structure. Fluorescence spectroscopy with a probe for hydrophobic protein regions showed that Zn2+ may affect the conformation of 21.5-kDa MBP in aqueous solution. Keywords: myelin basic protein (MBP); intrinsically-disordered protein; circular dichroism; fluoresence spectroscopy

Influence of Glycerol on the Structure and Thermal Stability of Lysozyme: A Dynamic Light Scattering and Circular Dichroism Study

2009 ◽  
Vol 113 (51) ◽  
pp. 16420-16424 ◽  
Author(s):  
Alessandro Esposito ◽  
Lucia Comez ◽  
Stefania Cinelli ◽  
Filippo Scarponi ◽  
Giuseppe Onori
Keyword(s):  
Thermal Stability ◽  
Circular Dichroism ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Circular Dichroïsm ◽  
Thermal Stability Of

scholarly journals Thermal stability analyses of human PERIOD-2 C-terminal domain using dynamic light scattering and circular dichroism

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0221180
Author(s):  
Yuejiao Xian ◽  
Brenda Moreno ◽  
Victoria Miranda ◽  
Neha Vijay ◽  
Luis C. Nunez ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Circular Dichroism ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Stability Analyses ◽  
Terminal Domain ◽  
Period 2 ◽  
Circular Dichroïsm
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