Near-infrared autofluorescence imaging of cutaneous melanins and human skin in vivo

2009 ◽  
Vol 14 (2) ◽  
pp. 024017 ◽  
Author(s):  
Xiao Han ◽  
Harvey Lui ◽  
David I. McLean ◽  
Haishan Zeng
Keyword(s):  
Human Skin ◽  
Near Infrared ◽  
Autofluorescence Imaging

Evaluation of variations of biomolecular constituents in human skin in vivo by near-infrared Raman spectroscopy

2001 ◽  
Author(s):  
Zhiwei Huang ◽  
Haishan Zeng ◽  
Calum E. MacAulay ◽  
Iltefat Hamzavi ◽  
David I. McLean ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Human Skin ◽  
Near Infrared

scholarly journals Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination

2019 ◽  
Vol 11 (523) ◽  
pp. eaay7162 ◽  
Author(s):  
Kevin J. McHugh ◽  
Lihong Jing ◽  
Sean Y. Severt ◽  
Mache Cruz ◽  
Morteza Sarmadi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Human Skin ◽  
Data Storage ◽  
Near Infrared ◽  
Learning Algorithm ◽  
Ex Vivo ◽  
Neutralizing Antibody ◽  
Nir Light ◽  
Antibody Titers

Accurate medical recordkeeping is a major challenge in many low-resource settings where well-maintained centralized databases do not exist, contributing to 1.5 million vaccine-preventable deaths annually. Here, we present an approach to encode medical history on a patient using the spatial distribution of biocompatible, near-infrared quantum dots (NIR QDs) in the dermis. QDs are invisible to the naked eye yet detectable when exposed to NIR light. QDs with a copper indium selenide core and aluminum-doped zinc sulfide shell were tuned to emit in the NIR spectrum by controlling stoichiometry and shelling time. The formulation showing the greatest resistance to photobleaching after simulated sunlight exposure (5-year equivalence) through pigmented human skin was encapsulated in microparticles for use in vivo. In parallel, microneedle geometry was optimized in silico and validated ex vivo using porcine and synthetic human skin. QD-containing microparticles were then embedded in dissolvable microneedles and administered to rats with or without a vaccine. Longitudinal in vivo imaging using a smartphone adapted to detect NIR light demonstrated that microneedle-delivered QD patterns remained bright and could be accurately identified using a machine learning algorithm 9 months after application. In addition, codelivery with inactivated poliovirus vaccine produced neutralizing antibody titers above the threshold considered protective. These findings suggest that intradermal QDs can be used to reliably encode information and can be delivered with a vaccine, which may be particularly valuable in the developing world and open up new avenues for decentralized data storage and biosensing.

A modified algorithm for continuous wave near infrared spectroscopy applied to in-vivo animal experiments and on human skin

2008 ◽  
Author(s):  
John H. G. M. Klaessens ◽  
Jeroen C. W. Hopman ◽  
K. Djien Liem ◽  
Rowland de Roode ◽  
Rudolf M. Verdaasdonk ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Human Skin ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Continuous Wave ◽  
Animal Experiments ◽  
Modified Algorithm

scholarly journals In vivo near-infrared autofluorescence imaging of retinal pigment epithelial cells with 757 nm excitation

2018 ◽  
Vol 9 (12) ◽  
pp. 5946 ◽  
Author(s):  
Kate Grieve ◽  
Elena Gofas-Salas ◽  
R. Daniel Ferguson ◽  
José Alain Sahel ◽  
Michel Paques ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Near Infrared ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Autofluorescence Imaging ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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