Back-illuminated electron multiplying technology: the world"s most sensitive CCD for ultralow-light microscopy

2003 ◽  
Author(s):  
Colin G. Coates ◽  
Donal J. Denvir ◽  
Emer Conroy ◽  
Noel G. McHale ◽  
Keith Thornbury ◽  
...  
Keyword(s):  
Light Microscopy ◽  
The World ◽  
Back Illuminated

Ultrasensitivity, speed, and resolution: optimizing low-light microscopy with the back-illuminated electron-multiplying CCD

2003 ◽  
Author(s):  
Colin G. Coates ◽  
Donal J. Denvir ◽  
Noel G. McHale ◽  
Keith D. Thornbury ◽  
Mark A. Hollywood
Keyword(s):  
Light Microscopy ◽  
Low Light ◽  
Back Illuminated

scholarly journals Tattoo ink nanoparticles in skin tissue and fibroblasts

2015 ◽  
Vol 6 ◽  
pp. 1183-1191 ◽  
Author(s):  
Colin A Grant ◽  
Peter C Twigg ◽  
Richard Baker ◽  
Desmond J Tobin
Keyword(s):  
Atomic Force Microscopy ◽  
Light Microscopy ◽  
Collagen Fibril ◽  
Chemical Activity ◽  
The West ◽  
Force Microscopy ◽  
Atomic Force ◽  
The World ◽  
The Subject ◽  
Tattoo Inks

Tattooing has long been practised in various societies all around the world and is becoming increasingly common and widespread in the West. Tattoo ink suspensions unquestionably contain pigments composed of nanoparticles, i.e., particles of sub-100 nm dimensions. It is widely acknowledged that nanoparticles have higher levels of chemical activity than their larger particle equivalents. However, assessment of the toxicity of tattoo inks has been the subject of little research and ink manufacturers are not obliged to disclose the exact composition of their products. This study examines tattoo ink particles in two fundamental skin components at the nanometre level. We use atomic force microscopy and light microscopy to examine cryosections of tattooed skin, exploring the collagen fibril networks in the dermis that contain ink nanoparticles. Further, we culture fibroblasts in diluted tattoo ink to explore both the immediate impact of ink pigment on cell viability and also to observe the interaction between particles and the cells.

scholarly journals The role of electron microscopy in kidney lesions: A review of its diagnostic importance

2013 ◽  
Vol 3 (5) ◽  
pp. 411-415
Author(s):  
AD Pant ◽  
K Solez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Renal Biopsy ◽  
Renal Pathology ◽  
Malignant Diseases ◽  
The World ◽  
Kidney Lesions ◽  
Diagnostic Importance

Electron microscopy is a technology which was at one time widely used for renal as well as non-renal benign and malignant diseases, but its use has been rapidly declining as hospitals all over the world cut down on expenses. This leaves the renal pathologist with only light microscopy and immunofl uorescence at his disposal to diagnose diseases. Few studies have stated the importance of electron microscopy in routine renal biopsy reporting. We look at different cases where electron microscopy has been helpful in diagnosis and review the literature to assess the role this investigative modality still has to play in modern renal pathology. Journal of Pathology of Nepal (2013) Vol. 3, No.1, Issue 5, 411-415 DOI: http://dx.doi.org/10.3126/jpn.v3i5.7871

Oncocytoma of the larynx

1990 ◽  
Vol 104 (4) ◽  
pp. 346-349 ◽  
Author(s):  
Anthony C. Robinson ◽  
Antonios Kaberos ◽  
Phillip M. Cox ◽  
Michael P. Stearns
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
World Literature ◽  
Histopathological Diagnosis ◽  
True Vocal Cord ◽  
Accurate Definition ◽  
Thyroid Glands ◽  
The World ◽  
Adequate Surgery ◽  
Definition Of

AbstractA case of oncocytoma of the larynx is presented. These lesions are rarely found in the larynx, and have been more commonly described in the salivary and thyroid glands. The histopathological diagnosis is often difficult and the light microscopy can be unreliable for oncocytic lesions. For a more accurate definition of these tumours, the use of histochemical stains together with electron microscopy are advocated. With adequate surgery, the prognosis is excellent, and tumours are unlikely to recur. This paper discusses the management of a patient with a benign oncocytoma of the true vocal cord, and presents a review of the world literature.

scholarly journals Formaldehyde as a Fixative for Light and Electron Microscopy

2000 ◽  
Vol 8 (5) ◽  
pp. 30-31
Author(s):  
Freida L. Carson
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Parallel Study ◽  
Electron Microscopic ◽  
Dual Purpose ◽  
Ultrastructural Morphology ◽  
The World ◽  
Selection Of

Since Blum discovered its hardening properties in 1893, formaldehyde has become the most widely used fixative in the world for specimens to be examined by light microscopy. However, since most commercial preparations of formaldehyde contain methanol, a protein precipitant, formaldehyde has been considered an unsatisfactory fixative for tissues to be examined by electron microscopy. In 1973, Carson et al., described a parallel study comparing the electron microscopic results of fixation with paraformaidehyde vs. formaldehyde. They found that there was no difference in the preservation of ultrastructural morphology provided that the buffer systems were identical. In 1976, McDowell and Trump described a fixative combining commercial formaldehyde and glutaraldehyde (4CF-1G). Both of these fixatives are dual purpose fixatives and preclude the selection of tissue for electron microscopy prior to fixation. They can both be prepared in large quantities and used for routine surgical specimens. The fixative containing formaldehyde alone does not need to be refrigerated and is stable for months; whereas, the formaldehyde-glutaraldehyde mixture should be refrigerated.

scholarly journals Trichodina colisae (Ciliophora: Trichodinidae): new parasite records for two freshwater fish species farmed in Brazil

2012 ◽  
Vol 21 (4) ◽  
pp. 366-371 ◽  
Author(s):  
Gabriela Tomas Jerônimo ◽  
Natália da Costa Marchiori ◽  
Santiago Benites de Pádua ◽  
José Dias Neto ◽  
Fabiana Pilarski ◽  
...  
Keyword(s):  
South America ◽  
Light Microscopy ◽  
Silver Nitrate ◽  
Fish Species ◽  
Piaractus Mesopotamicus ◽  
The World ◽  
First Occurrence ◽  
Taxonomic Studies ◽  
Important Fish ◽  
Ciliate Protozoa

Family Trichodinidae comprises ciliate protozoa distributed worldwide; they are considered some of the main parasitological agents infecting cultivated fish. However, the trichodinidae parasitizing important fish species cultured in Brazil are unknown, and more taxonomic studies on this group of parasites are required. This research morphologically characterizes Trichodina colisae Asmat & Sultana, (2005) of pacu (Piaractus mesopotamicus) and patinga hybrid (P. mesopotamicus × P. brachypomus) cultivated in the central and southeast regions of the country. Fresh assemblies were made from mucus scraped from the skin, fins and gills, fixed with methanol and, subsequently, impregnated with silver nitrate and stained with Giemsa for assessment under light microscopy. This research reports not only the second occurrence of T. colisae in the world, but also its first occurrence in South America.

Optimizing low-light microscopy with back-illuminated electron multiplying charge-coupled device: enhanced sensitivity, speed, and resolution

2004 ◽  
Vol 9 (6) ◽  
pp. 1244 ◽  
Author(s):  
Colin G. Coates ◽  
Donal J. Denvir ◽  
Noel G. McHale ◽  
Keith D. Thornbury ◽  
Mark A. Hollywood
Keyword(s):  
Light Microscopy ◽  
Low Light ◽  
Charge Coupled Device ◽  
Enhanced Sensitivity ◽  
Back Illuminated

scholarly journals Assessing morphological congruence in Dinobryon species and their stomatocysts, including a newly established Dinobryon pediforme–stomatocyst connection

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jolanta Piątek ◽  
Joanna Lenarczyk ◽  
Marcin Piątek
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Phylogenetic Relationships ◽  
Morphological Characters ◽  
Marine Waters ◽  
Resting Stages ◽  
The World ◽  
Scanning Electron ◽  
Sem Analyses

Abstract The chrysophyte genus Dinobryon Ehrenberg consists of 44 taxa, which occur in freshwaters, rarely marine waters, mostly in temperate regions of the world. The taxa of Dinobryon produce characteristic solitary or dendroid colonies and resting stages called stomatocysts. Only 20 Dinobryon taxa have information on produced stomatocysts and only four stomatocysts are reliably linked with vegetative stages using modern identification standards employing scanning electron microscopy (SEM) analyses. In this study, an encysted material of Dinobryon pediforme (Lemmermann) Steinecke was collected in two lakes in contrasting regions of Poland. Light microscopy (LM) and scanning electron microscopy (SEM) analyses revealed that Dinobryon pediforme produces stomatocyst #61, Piątek J. that is described here as new morphotype following the International Statospore Working Group (ISWG) guidelines. This raises to five the number of reliable links between vegetative stages of Dinobryon species and corresponding stomatocysts. Phenotypic similarities between Dinobryon species and their stomatocysts, analysed for five reliably established links, showed no relationships in size and shape between loricas and stomatocysts belonging to the same species. The morphological characters of loricas and stomatocysts mapped onto the phylogenetic tree of the five Dinobryon species revealed only little congruence between their morphology and phylogenetic relationships.

scholarly journals Formaldehyde as a Fixative for Light and Electron Microscopy

2000 ◽  
Vol 8 (3) ◽  
pp. 34-35
Author(s):  
Freida L. Carson
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Parallel Study ◽  
Electron Microscopic ◽  
Dual Purpose ◽  
Ultrastructural Morphology ◽  
The World ◽  
Selection Of

Since Blum discovered its hardening properties in 1893, formaldehyde has become the most widely used fixative in the world for specimens to be examined by light microscopy. However, since most commercial preparations of formaldehyde contain methanol, a protein precipitant, formaldehyde has been considered an unsatisfactory fixative for tissues to be examined by electron microscopy.In 1973, Carson et a/, described a parallel study comparing the electron microscopic results of fixation with paraformaldehyde vs. formaldehyde. They found that there was no difference in the preservation of ultrastructural morphology provided that the buffer systems were identical. In 1976, McDowell and Trump described a fixative combining commercial formaldehyde and glutaraldehyde (4CF-1G). Both of these fixatives are dual purpose fixatives and preclude the selection of tissue for electron microscopy prior to fixation. They can both be prepared in large quantities and used for routine surgical specimens.

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