Environmental DNA Monitoring of Biodiversity Hotspots in Danish Marine Waters

We investigated the use of eDNA metabarcoding for supplementing traditional diver-based monitoring of biodiversity of marine boulder reefs within the photic zone. The applied sampling design made it possible to evaluate the usefulness of eDNA monitoring as a supplement for traditional monitoring. Specifically, this study aimed to (1) assess the local influence of boulder reefs on biodiversity across the North Sea to Baltic Sea transition zone and (2) investigate the importance of environmental gradients for patterns in community structure. On samples collected during August 2020, we compared the composition and abundance of species associated with nine reefs, representing an environmental gradient of salinity (16–33 psu), water temperature (16–21°C) and water depth (6–29 m). At each reef site, water was sampled near the bottom just above the reef and on average 2.6 km upstream and downstream (location) and sequenced with metabarcoding using COI, 18S and 12S rDNA primers. eDNA identified 400 species, diver-based observations identified 184 with an overlap of 70 species (12%) and 81 genera (18%). While eDNA identified many infaunal species, it did not detect several macroalgal species which dominated in the diver-based observations. Multivariate analysis of eDNA and diver-based community structure both distinguished between reef communities, with a significant match between patterns observed by the two methods (r = 0.37, p = 0.02). Furthermore, the eDNA approach made it possible to identify significant differences in species composition between upstream, above-reef and downstream locations, suggesting that eDNA leaves a local footprint in benthic habitats. Patterns in both eDNA and diver-based species composition and richness were significantly related to geographical distance, salinity, water temperature and water depth. Despite of low detection of macroalgae, the eDNA sampling provided a substantial supplement to traditional diver-based monitoring of biodiversity around benthic hotspots in the Danish marine waters and therefore we recommend to add eDNA methods to conventional monitoring programs in the future.

Bacterial Shrimp Disease in Iraqi Marine Waters

A sample of 116 shrimps Metapenaeus affinis (H. Milne-Edwards, 1837) were collected from local fish markets in Basrah Province, Southern Iraq. Height, weight, and pathological signs of the shrimps were recorded. Both morphological and biochemical examination by VITEK identification system were undertaken. Pathological infections were seen on the cuticle of abdominal segments in addition to a severe infection in uropod (tail segment), rostrum and pleopods (walking legs). Results of biochemical identification showed the presence of Aeromonas sobria and A. salmonicida from the infected organs and this study is considered as the first record of this bacterial infection in shrimps in Iraq.

Evaluation For the Recovery Environmental the Beach Chorrillos of Huacho

The Chorrillos beach of the port of Huacho, is located north of Lima, Peru, and is contaminated by wastewater and solids, discharges by industry and population. For your evaluation of recovery environmental experimental research was applied using the following techniques: observation, environmental assessment, surveys, measurement of effluent flows and analysis of samples of its marine waters.

Early Pliocene Marine Transgression into the Lower Colorado River Valley, Southwestern USA, by Re-Flooding of a Former Tidal Strait

Marine straits and seaways are known to host a wide range of sedimentary processes and products, but the role of marine connections in the development of large river systems remains little studied. This study explores a hypothesis that shallow marine waters flooded the lower Colorado River valley at ∼ 5 Ma along a fault-controlled former tidal straight, soon after the river was first integrated to the northern Gulf of California. The upper bioclastic member of the southern Bouse Formation provides a critical test of this hypothesis. The upper bioclastic member contains wave ripple-laminated bioclastic grainstone with minor red mudstone, pebbly grainstone with HCS-like stratification and symmetrical gravelly ripples, and calcareous-matrix conglomerate. Fossils include upward-branching segmented coralline-like red algae with no known modern relatives but confirmed as marine calcareous algae, echinoid spines, barnacles, shallow marine foraminifers, clams, and serpulid worm tubes. These results provide evidence for deposition in a shallow marine bay or estuary seaward of the transgressive backstepping Colorado River delta. Tsunamis generated by seismic and meteorologic sources likely produced the HCS-like and wave-ripple cross-bedding in poorly-sorted gravelly grainstone. Marine waters inundated a former tidal strait within a fault-bounded tectonic lowland that connected the lower Colorado River to the Gulf of California. Delta backstepping and transgression resulted from a decrease in sediment output due to sediment trapping in upstream basins and relative sea-level rise produced by regional tectonic subsidence.Supplementary material at https://doi.org/10.6084/m9.figshare.c.5740426

From Inshore To Offshore: Microplastic Flows in Three Areas of The MSFD Italian Subregions

An increasing number of ecologists and researchers have addressed microplastics pollution in marine waters to explain the source, the transport, and the fate of these pollutants. Further data on the concentration of microplastics in the water column are crucial for understanding the impacts on ecosystems and to implement monitoring programs.This study provides information on the concentration and composition of sea water surface microplastics in three Italian subregions of the MSFD. We examined the flow of MPs from coastal to offshore areas, comparing their densities. We tested the efficiency of two sampling methodologies to evaluate the abundance and typology of MPs between marine layers. The results of this study confirm the high values of this pollutant in the Mediterranean Sea (0.029 ± 0.033 items · m-2), a MPs gradient from coastal to offshore areas, and a difference between the surface and subsurface marine layers.

Disinfection By-Products (DBPs) in Seawaters, Sediments and Biota near a Marine Terminal for Regasifying Liquefied Natural Gas (LNG) in the Northern Adriatic Sea (Italy)

In 2009, the first Italian offshore LNG terminal, located approximately 12 km from the coast of Italy in the Northern Adriatic Sea, started its operation phase. Even if the active chlorine concentration in the discharged seawater is within limits set by Italian regulations (0.2 mg/L), to verify the environmental impact of disinfection by-products (DBPs) on the ecosystem, a specific monitoring program was scheduled from 2010 to 2015. The present study is the first displaying results of DBPs in marine waters of the Adriatic Sea. During the first two years of monitoring activities, DBPs were slightly above limit of quantifications (LOQs) in all investigated matrices and limited to the nearest area around the terminal. In these surveys, bromoform was the most frequently detected compound in seawaters, while haloacetic acid presence, as well as transplanted mussels and fish fauna, mostly characterized sediments. In the following surveys, levels were mostly negligible in all matrices investigated, with values mostly below the specific LOQs of the different compounds.

First Record of Two Parasitic Helminths from Cobia Rachycentron canadum (L.) (Carangiformes, Rachycentridae) from Iraqi Marine Waters

A total of 14 specimens of Cobia Rachycentron canadum (L.) were caught from Iraqi marine waters during the period from October 2019 till May 2020. Two parasitic helminths (One species each of nematode and acanthocephalan) were isolated from alimentary canal of the cobia. These are the adults of Iheringascaris inquies (Linton, 1901) Deardorff & Overstreet, 1980 and Serrasentis sagittifer Linton, 1889. The record of these worms, from this fish, is considered as the first record in Iraq. Adequate description of both I. inquies and adult S. sagittifer from cobia for the first time in the Arabian Gulf is given.