Optimizing low-light microscopy with back-illuminated electron multiplying charge-coupled device: enhanced sensitivity, speed, and resolution

2004 ◽  
Vol 9 (6) ◽  
pp. 1244 ◽  
Author(s):  
Colin G. Coates ◽  
Donal J. Denvir ◽  
Noel G. McHale ◽  
Keith D. Thornbury ◽  
Mark A. Hollywood
Keyword(s):  
Light Microscopy ◽  
Low Light ◽  
Charge Coupled Device ◽  
Enhanced Sensitivity ◽  
Back Illuminated

Ultrasensitivity, speed, and resolution: optimizing low-light microscopy with the back-illuminated electron-multiplying CCD

2003 ◽  
Author(s):  
Colin G. Coates ◽  
Donal J. Denvir ◽  
Noel G. McHale ◽  
Keith D. Thornbury ◽  
Mark A. Hollywood
Keyword(s):  
Light Microscopy ◽  
Low Light ◽  
Back Illuminated

scholarly journals Modular platform for low-light microscopy

2015 ◽  
Vol 6 (11) ◽  
pp. 4585 ◽  
Author(s):  
Tae Jin Kim ◽  
Silvan Tuerkcan ◽  
Andrew Ceballos ◽  
Guillem Pratx
Keyword(s):  
Light Microscopy ◽  
Low Light ◽  
Modular Platform

Electronic Cameras for Low‐Light Microscopy

2007 ◽  
pp. 219-249 ◽  
Author(s):  
Ivan Rasnik ◽  
Todd French ◽  
Ken Jacobson ◽  
Keith Berland
Keyword(s):  
Light Microscopy ◽  
Low Light

Electronic Cameras for Low-Light Microscopy

2003 ◽  
pp. 103-132 ◽  
Author(s):  
Keith Berland ◽  
Ken Jacobson ◽  
Todd French ◽  
Zenon Rajfur
Keyword(s):  
Light Microscopy ◽  
Low Light

Chapter 2 Electronic Cameras for Low-Light Microscopy

1998 ◽  
pp. 19-44 ◽  
Author(s):  
Keith Berland ◽  
Ken Jacobson ◽  
Todd French
Keyword(s):  
Light Microscopy ◽  
Low Light

scholarly journals Characterizing and correcting camera noises in back-illuminated sCMOS cameras

2021 ◽  
Author(s):  
Zhaoning Zhang ◽  
Yujie Wang ◽  
Rafael Piestun ◽  
Zhen-Li Huang
Keyword(s):  
Single Molecule ◽  
Low Cost ◽  
Large Field ◽  
Low Light ◽  
Conventional Microscopy ◽  
Application Fields ◽  
Imaging Performance ◽  
The Impact ◽  
Back Illuminated ◽  
Single Molecule Localization Microscopy

AbstractWith promising properties of fast imaging speed, large field-of-view, relative low cost and many others, back-illuminated sCMOS cameras have been receiving intensive attentions for low-light imaging in the past several years. However, due to the pixel-to-pixel difference of camera noises (called noise non-uniformity) in sCMOS cameras, researchers may hesitate to use them in some application fields, and sometimes wonder whether they should optimize the noise non-uniformity of their sCMOS cameras before using them in a specific application scenario. In this paper, we systematically characterize the impact of different types of sCMOS noises on image quality and perform corrections to these sCMOS noises. We verify that it is possible to use appropriate correction methods to push the non-uniformity of major camera noises, including readout noise, offset, and photon response, to a satisfactory level for conventional microscopy and single molecule localization microscopy. We further find out that, after these corrections, global read noise becomes a major concern that limits the imaging performance of back-illuminated sCMOS cameras. We believe this study provides new insights into the understanding of camera noises in back-illuminated sCMOS cameras, and also provides useful information for future development of this promising camera technology.

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