Noncontact three-dimensional surface profiling using reflected-light holographic confocal microscopy

2003 ◽  
Author(s):  
Irena Antosova ◽  
Zdenek Harna
Keyword(s):  
Confocal Microscopy ◽  
Three Dimensional ◽  
Surface Profiling ◽  
Reflected Light ◽  
Dimensional Surface

Morphology of Foliar Trichomes of the Chinese Cork OakQuercus variabilisby Electron Microscopy and Three-Dimensional Surface Profiling

2011 ◽  
Vol 17 (3) ◽  
pp. 461-468 ◽  
Author(s):  
Ki Woo Kim ◽  
Do-Hyun Cho ◽  
Pan-Gi Kim
Keyword(s):  
Electron Microscopy ◽  
Three Dimensional ◽  
Abaxial Surface ◽  
Single Row ◽  
Adaxial Surface ◽  
Surface Profiling ◽  
Transmission Electron ◽  
Ultrastructural Characterization ◽  
Disturbance Factor ◽  
Dimensional Surface

AbstractMorphology of foliar trichomes was analyzed inQuercus variabilisby electron microscopy and three-dimensional surface profiling. Leaves from suppressed or dominant sprouts of the oak species were collected after a forest fire to unravel the effects of the disturbance factor on sprouting of the oak species. Scanning electron microscopy revealed two types of trichomes depending on the leaf surface. The trichomes on the adaxial surface were branched and constricted, and possessed a single row of thin-walled cells with a collapsed morphology (glandular branched uniseriate trichomes). Meanwhile, the trichomes on the abaxial surface were star-shaped, unfused with each other, and had 6 to 10 rays (nonglandular simple stellate trichomes). An apparent proliferation of trichomes was evident on the adaxial surface of the dominant sprouts. Uniseriate trichomes could be discernable as an elevation from the surface by white light scanning interferometry. By transmission electron microscopy, thin and convoluted cell wall, degenerated cytoplasm, and a single row of cells were characteristic of the trichomes on the adaxial surface. The thick cell walls of the mature trichomes on the abaxial surface represented the nonglandular nature. This is the first report on the morphological and ultrastructural characterization of foliar trichomes of the oak species.

scholarly journals Comb-calibrated laser ranging for three-dimensional surface profiling with micrometer-level precision at a distance

2014 ◽  
Vol 22 (21) ◽  
pp. 24914 ◽  
Author(s):  
E. Baumann ◽  
F. R. Giorgetta ◽  
J.-D. Deschênes ◽  
W. C. Swann ◽  
I. Coddington ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Laser Ranging ◽  
Surface Profiling ◽  
Dimensional Surface

Three-dimensional visualization of the eye

1992 ◽  
Vol 50 (1) ◽  
pp. 586-587
Author(s):  
Barry R. Masters
Keyword(s):  
High Resolution ◽  
Confocal Microscopy ◽  
Volume Rendering ◽  
Three Dimensional ◽  
Full Thickness ◽  
Rabbit Cornea ◽  
Rabbit Eye ◽  
Reflected Light ◽  
Dimensional Reconstruction

The structure of the in situ rabbit cornea can be observed at high resolution and contrast with reflected light confocal microscopy. This paper describes the three dimensional reconstruction of the in situ cornea from an enucleated rabbit eye with confocal reflected light microscopy and volume rendering computer techniques. This paper shows the 3-D reconstruction of the full thickness of the cornea based on cubic voxels.

Diffraction-Assisted Image Correlation for Three-Dimensional Surface Profiling

2014 ◽  
Vol 55 (1) ◽  
pp. 155-165 ◽  
Author(s):  
Z. Pan ◽  
S. Xia ◽  
A. Gdoutou ◽  
G. Ravichandran
Keyword(s):  
Three Dimensional ◽  
Image Correlation ◽  
Surface Profiling ◽  
Dimensional Surface

scholarly journals Three-dimensional surface profiling and optical characterization of liquid microlens using a Shack–Hartmann wave front sensor

2011 ◽  
Vol 98 (17) ◽  
pp. 171104 ◽  
Author(s):  
Chenhui Li ◽  
Gunnsteinn Hall ◽  
Xuefeng Zeng ◽  
Difeng Zhu ◽  
Kevin Eliceiri ◽  
...  
Keyword(s):  
Wave Front ◽  
Three Dimensional ◽  
Optical Characterization ◽  
Surface Profiling ◽  
Dimensional Surface

scholarly journals Swept-Source-Based Chromatic Confocal Microscopy

Sensors ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 7347
Author(s):  
Dawoon Jeong ◽  
Se Jin Park ◽  
Hansol Jang ◽  
Hyunjoo Kim ◽  
Jaesun Kim ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
High Speed ◽  
Imaging System ◽  
Three Dimensional ◽  
Chromatic Aberration ◽  
Cover Glass ◽  
Focal Distance ◽  
Swept Source ◽  
Surface Profiling ◽  
Novel Concept

Chromatic confocal microscopy (CCM) has been intensively developed because it can exhibit effective focal position scanning based on the axial chromatic aberration of broadband light reflected from a target. To improve the imaging speed of three-dimensional (3D) surface profiling, we have proposed the novel concept of swept-source-based CCM (SS-CCM) and investigated the usefulness of the corresponding imaging system. Compared to conventional CCM based on a broadband light source and a spectrometer, a swept-source in the proposed SS-CCM generates light with a narrower linewidth for higher intensity, and a single photodetector employed in the system exhibits a fast and sensitive response by immediately obtaining spectrally encoded depth from a chromatic dispersive lens array. Results of the experiments conducted to test the proposed SS-CCM system indicate that the system exhibits an axial chromatic focal distance range of approximately 360 μm for the 770–820 nm swept wavelength range. Moreover, high-speed surface profiling images of a cover glass and coin were successfully obtained with a short measurement time of 5 ms at a single position.

Confocal laser microscopy of impregnated central nervous system tissue

1988 ◽  
Vol 46 ◽  
pp. 94-95
Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Confocal Laser ◽  
High Contrast ◽  
Central Nervous System Tissue ◽  
Tissue Samples ◽  
Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

Laser Scanning Confocal Microscopy and Three-Dimesnsional Reconstruction of the Mitotic Spindles of Unequally Dividing Embryonic Cells

1990 ◽  
Vol 48 (3) ◽  
pp. 166-167
Author(s):  
J. Holy ◽  
G. Schatten
Keyword(s):  
Confocal Microscopy ◽  
Mitotic Spindle ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Two Dimensions ◽  
Embryonic Cells ◽  
Mitotic Spindles ◽  
Cleavage Division ◽  
Scanning Confocal Microscopy

One of the classic limitations of light microscopy has been the fact that three dimensional biological events could only be visualized in two dimensions. Recently, this shortcoming has been overcome by combining the technologies of laser scanning confocal microscopy (LSCM) and computer processing of microscopical data by volume rendering methods. We have employed these techniques to examine morphogenetic events characterizing early development of sea urchin embryos. Specifically, the fourth cleavage division was examined because it is at this point that the first morphological signs of cell differentiation appear, manifested in the production of macromeres and micromeres by unequally dividing vegetal blastomeres.The mitotic spindle within vegetal blastomeres undergoing unequal cleavage are highly polarized and develop specialized, flattened asters toward the micromere pole. In order to reconstruct the three-dimensional features of these spindles, both isolated spindles and intact, extracted embryos were fluorescently labeled with antibodies directed against either centrosomes or tubulin.

Enhancement of Heat Transfer Performance in Nuclear Fuel Rod Using Nanofluids and Surface Roughness Technique

2015 ◽  
Author(s):  
Kang Liu ◽  
Titan C. Paul ◽  
Leo A. Carrilho ◽  
Jamil A. Khan
Keyword(s):  
Heat Transfer ◽  
Surface Roughness ◽  
Heat Transfer Coefficient ◽  
Transfer Coefficient ◽  
Nuclear Fuel ◽  
Three Dimensional ◽  
Pressurized Water ◽  
Bulk Fluid ◽  
Fuel Rod ◽  
Dimensional Surface

The experimental investigations were carried out of a pressurized water nuclear reactor (PWR) with enhanced surface using different concentration (0.5 and 2.0 vol%) of ZnO/DI-water based nanofluids as a coolant. The experimental setup consisted of a flow loop with a nuclear fuel rod section that was heated by electrical current. The fuel rod surfaces were termed as two-dimensional surface roughness (square transverse ribbed surface) and three-dimensional surface roughness (diamond shaped blocks). The variation in temperature of nuclear fuel rod was measured along the length of a specified section. Heat transfer coefficient was calculated by measuring heat flux and temperature differences between surface and bulk fluid. The experimental results of nanofluids were compared with the coolant as a DI-water data. The maximum heat transfer coefficient enhancement was achieved 33% at Re = 1.15 × 105 for fuel rod with three-dimensional surface roughness using 2.0 vol% nanofluids compared to DI-water.

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