Image reconstruction for three-dimensional transmitted-light DIC microscopy

1997 ◽  
Author(s):  
Chrysanthe Preza ◽  
Donald L. Snyder ◽  
Jose-Angel Conchello
Keyword(s):  
Image Reconstruction ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Dic Microscopy

Three dimensional deblurring of transmitted-light brightfield micrographs

1993 ◽  
Vol 51 ◽  
pp. 564-565
Author(s):  
Santosh Bhattacharyya
Keyword(s):  
Image Reconstruction ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Reconstruction Algorithms ◽  
3D Image Reconstruction ◽  
Structural Details ◽  
Spread Function ◽  
Early Testing ◽  
Linearized Model ◽  
Image Reconstruction Algorithms

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.

Imaging models for three-dimensional transmitted-light DIC microscopy

1996 ◽  
Author(s):  
Chrysanthe Preza ◽  
Donald L. Snyder ◽  
Jose-Angel Conchello
Keyword(s):  
Three Dimensional ◽  
Transmitted Light ◽  
Dic Microscopy

Criteria and Methods for Reliable Three-Dimensional Image Reconstruction

1974 ◽  
Vol 32 ◽  
pp. 330-331
Author(s):  
R. A. Crowther
Keyword(s):  
Image Reconstruction ◽  
Finite Number ◽  
Density Distribution ◽  
Electron Micrographs ◽  
Mathematical Problem ◽  
Three Dimensional ◽  
Ideal Case ◽  
Dimensional Image ◽  
General Object ◽  
The Ideal

The reconstruction of a three-dimensional image of a specimen from a set of electron micrographs reduces, under certain assumptions about the imaging process in the microscope, to the mathematical problem of reconstructing a density distribution from a set of its plane projections.In the absence of noise we can formulate a purely geometrical criterion, which, for a general object, fixes the resolution attainable from a given finite number of views in terms of the size of the object. For simplicity we take the ideal case of projections collected by a series of m equally spaced tilts about a single axis.

Enhanced Three-Dimensional Color Conversion Based on Discrete Wavelet Transform

2012 ◽  
Vol 155-156 ◽  
pp. 440-444
Author(s):  
He Yan ◽  
Xiu Feng Wang
Keyword(s):  
Information Technology ◽  
Wavelet Transform ◽  
Image Reconstruction ◽  
Image Quality ◽  
Image Compression ◽  
High Performance ◽  
Three Dimensional ◽  
Discrete Wavelet ◽  
Compression Algorithms ◽  
Color Conversion

JPEG2000 algorithm has been developed based on the DWT techniques, which have shown how the results achieved in different areas in information technology can be applied to enhance the performance. Lossy image compression algorithms sacrifice perfect image reconstruction in favor of decreased storage requirements. Wavelets have become a popular technology for information redistribution for high-performance image compression algorithms. Lossy compression algorithms sacrifice perfect image reconstruction in favor of improved compression rates while minimizing image quality lossy.

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

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