Imaging models for three-dimensional transmitted-light DIC microscopy

1996 ◽  
Author(s):  
Chrysanthe Preza ◽  
Donald L. Snyder ◽  
Jose-Angel Conchello
Keyword(s):  
Three Dimensional ◽  
Transmitted Light ◽  
Dic Microscopy

Three dimensional deblurring of transmitted-light brightfield micrographs

1993 ◽  
Vol 51 ◽  
pp. 564-565
Author(s):  
Santosh Bhattacharyya
Keyword(s):  
Image Reconstruction ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Reconstruction Algorithms ◽  
3D Image Reconstruction ◽  
Structural Details ◽  
Spread Function ◽  
Early Testing ◽  
Linearized Model ◽  
Image Reconstruction Algorithms

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

scholarly journals High-throughput in-focus differential interference contrast imaging of three-dimensional orientations of single gold nanorods coated with a mesoporous silica shell

RSC Advances ◽  
2020 ◽  
Vol 10 (50) ◽  
pp. 29868-29872
Author(s):  
Geun Wan Kim ◽  
Seokyoung Yoon ◽  
Jung Heon Lee ◽  
Ji Won Ha
Keyword(s):  
Mesoporous Silica ◽  
High Throughput ◽  
Gold Nanorods ◽  
Focal Plane ◽  
Three Dimensional ◽  
Silica Shell ◽  
Differential Interference Contrast ◽  
Contrast Imaging ◽  
3D Space ◽  
Dic Microscopy

Spherical AuNRs@mSiO2 have randomly oriented AuNR cores in 3D space, which could be resolved on the same focal plane by interference-based DIC microscopy.

Studies onTernidens deminutusRailliet & Henry, 1909 (Nematoda) I. External morphology

1973 ◽  
Vol 47 (2) ◽  
pp. 119-126 ◽  
Author(s):  
J. M. Goldsmid ◽  
N. F. Lyons
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Size Range ◽  
Three Dimensional ◽  
Transmitted Light ◽  
External Morphology ◽  
Morphological Studies ◽  
Transmitted Light Microscopy ◽  
Dimensional Picture ◽  
Scanning Electron

The present paper describes the size range ofTernidens deminutusfrom human and baboon hosts in Rhodesia and discusses the possible reasons for the differences noted.Using transmitted light and the scanning electron microscope, the external morphology ofT. deminutushas been re-studied and compared to investigations by other authors using transmitted light microscopy alone.The paper also illustrates the value of the scanning electron microscope in morphological studies in helminthology, especially when used in conjunction with the light microscope, to give an excellent three-dimensional picture of the species under investigation.It is intended to follow this work with further studies on the anatomy, histology, ultrastructure and histochemistry ofTernidens deminutus.

Computational Model of DIC Microscopy for Reconstructing 3-D Specimens

2000 ◽  
Vol 39 (02) ◽  
pp. 105-109 ◽  
Author(s):  
F. Lanni ◽  
T. Kanade ◽  
F. Kagalwala
Keyword(s):  
Three Dimensional ◽  
Image Data ◽  
Visualization Tool ◽  
Differential Interference Contrast ◽  
Real Image ◽  
Biological Cells ◽  
Virtual Objects ◽  
Object Properties ◽  
Simulated Images ◽  
Dic Microscopy

Abstract:Differential Interference Contrast (DIC) microscopy is a powerful visualization tool used to study live biological cells. Its use, however, has been limited to qualitative observations. The inherent non-linear relation between the object properties and the image intensity makes quantitative analysis difficult. As a first step towards measuring optical properties of objects from DIC images, we develop a model for the image formation process using methods consistent with energy conservation laws. We verify our model by comparing real image data of manufactured specimens to simulated images of virtual objects. As the next step, we plan to use this model to reconstruct the three-dimensional properties of unknown specimens.

scholarly journals Portuguese marine fungi and the contribution of differential interference contrast (DIC) microscopy for their morphological identification

2013 ◽  
Vol 19 (S4) ◽  
pp. 17-18
Author(s):  
E. Azevedo ◽  
M.F. Caeiro ◽  
M. Barata
Keyword(s):  
Salt Marshes ◽  
Marine Fungi ◽  
Three Dimensional ◽  
Morphological Diversity ◽  
Sandy Beaches ◽  
Morphological Identification ◽  
Differential Interference Contrast ◽  
Initial Examination ◽  
Bright Field ◽  
Dic Microscopy

Marine fungi occur either in Open Ocean or in the intertidal zone of sandy beaches, salt marshes and mangroves, where their hosts and substrates are found. The development of morphological adaptations like appendages and sheaths of the spores are vital to the settlement and attachment to substrate surfaces, floatation and dispersion on seawater. The morphological features of these appendages and sheaths of spores also have an important role in the identification of marine fungi. Differential interference contrast (DIC) microscopy is an essential tool for the observation of these mucilaginous structures in marine fungi spores and was therefore applied to marine mycota from surveys along the Portuguese coast.Since 1991 Portuguese marine fungi have been studied and characterized based on morphological identifications. The studied environments included salt marshes, sandy beaches and marinas. On these environments different substrates were collected such as plants and baits of Spartina maritima, different categories of drift substrates and Fagus sylvatica and Pinus pinaster baits. These substrates, which had been exposed to different conditions of permanent and temporary submersion, were subjected to an initial examination under the stereoscope microscope in order to detect fruit bodies and spores of marine fungi. These structures were then observed in order to achieve the taxonomic identifications of these fungi, based on dichotomous keys for marine fungi.To observe and characterize the wide variety of appendages and mucilaginous sheaths of the ascospores, basidiospores and conidia often invisible in the bright field of the light microscope, the use of DIC microscopy was implemented, because three-dimensional images are produced, highlighting them. The identified fungi were microphotographed with a Leica Wild MPS 52 with Fujichrome RTP- 135, 64T Tungsten.The studies carried out with substrates subjected to conditions of permanent submersion highlighted the dominance of Ascomycota with unitunicate asci. The unitunicate asci are thin - walled, persistent or early deliquescing, favoring ascospores release on marine environmental conditions (fig 1a, 1b and 1c). On the other hand, Ascomycota with bitunicate asci, were mainly detected on temporary submersion conditions. These fungi presented asci with active spore discharge and mucilaginous sheaths that contributed to substrate attachment (fig. 2a and 2b). Ascospores and conidia presented morphological diversity on their appendages, which has particular importance on their success in marine environment (figs. 3, 4, 5, and 6).Marine Mycologists are in agreement that the best technique to observe the appendages and sheaths of spores, often invisible in the bright-field microscope is the use of differential interference or phase contrast microscopy. DIC microscopy was then applied to the observation of Portuguese marine fungi enabling to thoroughly characterize the structures essential to their morphological identifications.

Automated Three-Dimensional Tracing of Neurons in Confocal and Brightfield Images

2003 ◽  
Vol 9 (4) ◽  
pp. 296-310 ◽  
Author(s):  
Wenyun He ◽  
Thomas A. Hamilton ◽  
Andrew R. Cohen ◽  
Timothy J. Holmes ◽  
Christopher Pace ◽  
...  
Keyword(s):  
Image Analysis ◽  
Confocal Microscopy ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Graph Representation ◽  
Confocal Image ◽  
Collapse Prevention ◽  
Dye Penetration ◽  
Fluorescence Confocal Microscopy ◽  
Large N

Automated three-dimensional (3-D) image analysis methods are presented for tracing of dye-injected neurons imaged by fluorescence confocal microscopy and HRP-stained neurons imaged by transmitted-light brightfield microscopy. An improved algorithm for adaptive 3-D skeletonization of noisy images enables the tracing. This algorithm operates by performing connectivity testing over large N × N × N voxel neighborhoods exploiting the sparseness of the structures of interest, robust surface detection that improves upon classical vacant neighbor schemes, improved handling of process ends or tips based on shape collapse prevention, and thickness-adaptive thinning. The confocal image stacks were skeletonized directly. The brightfield stacks required 3-D deconvolution. The results of skeletonization were analyzed to extract a graph representation. Topological and metric analyses can be carried out using this representation. A semiautomatic method was developed for reconnection of dendritic fragments that are disconnected due to insufficient dye penetration, an imaging deficiency, or skeletonization errors.

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