Quantification of tryptophan in different layers of oral cancer tissue using fluorescence polarization technique

2021 ◽  
Author(s):  
Einstein Gnanatheepam ◽  
Anandh Sundaramoorthy ◽  
Udayakumar Kanniyappan ◽  
Koteeswaran Dornadula ◽  
Bharanidaran Ganesan ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Fluorescence Polarization ◽  
Cancer Tissue ◽  
Polarization Technique

scholarly journals Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM

The Analyst ◽  
2021 ◽  
Author(s):  
Barnaby Ellis ◽  
Conor A Whitley ◽  
Safaa Al Jedani ◽  
Caroline Smith ◽  
Philip Gunning ◽  
...  
Keyword(s):  
Machine Learning ◽  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Lymphoid Tissue ◽  
Learning Algorithm ◽  
Cancer Tissue ◽  
Machine Learning Algorithm ◽  
Nodal Metastases ◽  
Insight Into

A novel machine learning algorithm is shown to accurately discriminate between oral squamous cell carcinoma (OSCC) nodal metastases and surrounding lymphoid tissue on the basis of a single metric, the...

Synchronous Luminescence Spectroscopy as a Tool in the Discrimination and Characterization of Oral Cancer Tissue

2019 ◽  
Vol 29 (2) ◽  
pp. 361-367 ◽  
Author(s):  
Einstein Gnanatheepam ◽  
Udayakumar Kanniyappan ◽  
Koteeswaran Dornadula ◽  
Aruna Prakasarao ◽  
Ganesan Singaravelu
Keyword(s):  
Oral Cancer ◽  
Luminescence Spectroscopy ◽  
Cancer Tissue ◽  
Synchronous Luminescence Spectroscopy

Assay of an antitumor protein, neocarzinostatin, and its antibody by fluorescence polarization.

1978 ◽  
Vol 24 (12) ◽  
pp. 2139-2144 ◽  
Author(s):  
H Maeda
Keyword(s):  
Pharmacokinetic Parameter ◽  
Fluorescence Polarization ◽  
Fluorescein Isothiocyanate ◽  
Apparent Volume ◽  
Volume Of Distribution ◽  
Radial Immunodiffusion ◽  
Antitumor Antibiotic ◽  
Single Radial Immunodiffusion ◽  
Polarization Technique ◽  
Apparent Volume Of Distribution

Abstract I evaluated use of the fluorescence polarization technique to measure neocarzinostatin, a proteinaceous antitumor antibiotic, and its antibody, in serum. The antigen (neocarzinostatin), labeled with fluorescein isothiocyanate, was allowed to interact with its antibody in a cuvet, in the instrument, yielding an increase in the fluorescence polarization value. Antibody content was determined in the presence of a definite amount of the labeled antigen, fluorescence polarization values increasing in parallel with each addition of antibody. Antigen content was determined with a known amount of antibody, which reacted at first with an unknown amount of antigen in samples, followed by addition of a definite amount of the labeled antigen (competition). I used the method to determine a pharmacokinetic parameter, the apparent volume of distribution for neocarzinostatin in rabbits, using drug-injected rabbit sera. I evaluated precision, accuracy, and reproducibility, using various samples or possible interfering substances such as bilirubin and hemoglobin, and also compared results for antigen with those by single radial immunodiffusion assay. The present assay is fast (less than 2 min), sensitive (less than 10 nmol/liter can be detected), and simple (there is no separation step before readout of the results).

The cyclo-oxygenase and lipoxygenase pathways in human oral cancer tissue

1984 ◽  
Vol 12 ◽  
pp. 145-147 ◽  
Author(s):  
Hubert Porteder ◽  
Michael Matejka ◽  
Walter Ulrich ◽  
Helmut Sinzinger
Keyword(s):  
Oral Cancer ◽  
Cancer Tissue

scholarly journals Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ping-Ho Chen ◽  
Ka-Wo Lee ◽  
Cheng-Chieh Hsu ◽  
Jeff Yi-Fu Chen ◽  
Yan-Hsiung Wang ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Splice Variant ◽  
Full Length ◽  
Betel Quid ◽  
Human Gingival Fibroblast ◽  
Cancer Tissue ◽  
Gingival Fibroblast ◽  
Dependent Manner ◽  
Pharyngeal Cancer ◽  
Oral And Pharyngeal Cancer

Betel quid (BQ) is a psychostimulant, an addictive substance, and a group 1 carcinogen that exhibits the potential to induce adverse health effects. Approximately, 600 million users chew a variety of BQ. Areca nut (AN) is a necessary ingredient in BQ products. Arecoline is the primary alkaloid in the AN and can be metabolized through the cytochrome P450 (CYP) superfamily by inducing reactive oxygen species (ROS) production. Full-length CYP26B1 is related to the development of oral pharyngeal cancers. We investigated whether a splice variant of CYP26B1 is associated with the occurrence of ROS related oral and pharyngeal cancer. Cytotoxicity assays were used to measure the effects of arecoline on cell viability in a dose-dependent manner.In vitroandin vivostudies were conducted to evaluate the expression of the CYP26B1 splice variant. The CYP26B1 splice variant exhibited lower expression than did full-length CYP26B1 in the human gingival fibroblast-1 and Ca9-22 cell models. Increased expression of the CYP26B1 splice variant was observed in human oral cancer tissue compared with adjacent normal tissue, and increased expression was observed in patients at a late tumor stage. Our results suggested that the CYP26B1 splice variant is associated with the occurrence of BQ-related oral cancer.

scholarly journals Development of oral cancer tissue-mimicking phantom based on polyvinyl chloride plastisol and graphite for terahertz frequencies

2020 ◽  
Vol 25 (12) ◽  
Author(s):  
Tianmiao Zhang ◽  
Ravshanjon Nazarov ◽  
Alexey P. Popov ◽  
Petr S. Demchenko ◽  
Alexander V. Bykov ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Polyvinyl Chloride ◽  
Cancer Tissue ◽  
Terahertz Frequencies
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