Detection of DNA hybridization with immobilized fluorescently labeled oligonucleotide probes using fluorescence polarization technique

1999 ◽  
Author(s):  
Satoshi Abe ◽  
Shuji Toyonaga
Keyword(s):  
Dna Hybridization ◽  
Fluorescence Polarization ◽  
Oligonucleotide Probes ◽  
Polarization Technique

Assay of an antitumor protein, neocarzinostatin, and its antibody by fluorescence polarization.

1978 ◽  
Vol 24 (12) ◽  
pp. 2139-2144 ◽  
Author(s):  
H Maeda
Keyword(s):  
Pharmacokinetic Parameter ◽  
Fluorescence Polarization ◽  
Fluorescein Isothiocyanate ◽  
Apparent Volume ◽  
Volume Of Distribution ◽  
Radial Immunodiffusion ◽  
Antitumor Antibiotic ◽  
Single Radial Immunodiffusion ◽  
Polarization Technique ◽  
Apparent Volume Of Distribution

Abstract I evaluated use of the fluorescence polarization technique to measure neocarzinostatin, a proteinaceous antitumor antibiotic, and its antibody, in serum. The antigen (neocarzinostatin), labeled with fluorescein isothiocyanate, was allowed to interact with its antibody in a cuvet, in the instrument, yielding an increase in the fluorescence polarization value. Antibody content was determined in the presence of a definite amount of the labeled antigen, fluorescence polarization values increasing in parallel with each addition of antibody. Antigen content was determined with a known amount of antibody, which reacted at first with an unknown amount of antigen in samples, followed by addition of a definite amount of the labeled antigen (competition). I used the method to determine a pharmacokinetic parameter, the apparent volume of distribution for neocarzinostatin in rabbits, using drug-injected rabbit sera. I evaluated precision, accuracy, and reproducibility, using various samples or possible interfering substances such as bilirubin and hemoglobin, and also compared results for antigen with those by single radial immunodiffusion assay. The present assay is fast (less than 2 min), sensitive (less than 10 nmol/liter can be detected), and simple (there is no separation step before readout of the results).

Rapid, non-separation electrochemiluminescent DNA hybridization assays for PCR products, using 3′-labelled oligonucleotide probes

1992 ◽  
Vol 6 (6) ◽  
pp. 495-503 ◽  
Author(s):  
Satyanarayana R. Gudibande ◽  
John H. Kenten ◽  
John Link ◽  
Kenneth Friedman ◽  
Richard J. Massey
Keyword(s):  
Dna Hybridization ◽  
Oligonucleotide Probes ◽  
Pcr Products ◽  
Hybridization Assays

Specific liquid DNA hybridization kinetics measured by fluorescence polarization

1991 ◽  
Vol 244 ◽  
pp. 207-213 ◽  
Author(s):  
Thierry Herning ◽  
Eiichi Tamiya ◽  
Isao Karube ◽  
Shoji Kobayashi
Keyword(s):  
Dna Hybridization ◽  
Fluorescence Polarization

Palytoxin detection and quantification using the fluorescence polarization technique

2012 ◽  
Vol 424 (1) ◽  
pp. 64-70 ◽  
Author(s):  
A. Alfonso ◽  
A. Fernández-Araujo ◽  
C. Alfonso ◽  
B. Caramés ◽  
A. Tobio ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Polarization Technique ◽  
Detection And Quantification

Fluorescence polarization technique: a new method for vegetable oils classification

2015 ◽  
Vol 7 (12) ◽  
pp. 5175-5179 ◽  
Author(s):  
Taotao Mu ◽  
Siying Chen ◽  
Yinchao Zhang ◽  
Fandong Meng ◽  
Pan Guo ◽  
...  
Keyword(s):  
Vegetable Oils ◽  
Fluorescence Polarization ◽  
Edible Oils ◽  
Edible Oil ◽  
New Method ◽  
Oil Analysis ◽  
Polarization Of Fluorescence ◽  
Polarization Technique ◽  
Edible Oil Analysis ◽  
Favorable Conditions

Polarization of fluorescence is firstly employed in edible oil analysis. It is found that the degrees of polarization of edible oils are distinct from each other which provides favorable conditions for edible oil classification.

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