Pharmacology of near infrared nerve-specific fluorophores for fluorescence-guided nerve sparing surgical procedures

2021 ◽  
Author(s):  
Connor W. Barth ◽  
Lei G. Wang ◽  
Antonio Montaño ◽  
Alexander L. Antaris ◽  
Jonathan M. Sorger ◽  
...  
Keyword(s):  
Surgical Procedures ◽  
Near Infrared ◽  
Nerve Sparing

Lead Optimization of Nerve-Specific Fluorophores for Image-Guided Nerve Sparing Surgical Procedures

2021 ◽  
Author(s):  
Connor W. Barth ◽  
Lei G. Wang ◽  
Antonio Montano ◽  
Alexander L. Antaris ◽  
Alwin Klaassen ◽  
...  
Keyword(s):  
Surgical Procedures ◽  
Lead Optimization ◽  
Nerve Sparing ◽  
Image Guided

scholarly journals Real-time, functional intra-operative localization of rat cavernous nerve network using near-infrared cyanine voltage-sensitive dye imaging

2019 ◽  
Author(s):  
Jeeun Kang ◽  
Hanh N. D. Le ◽  
Serkan Karakus ◽  
Adarsha P. Malla ◽  
Maged M. Harraz ◽  
...  
Keyword(s):  
Radical Prostatectomy ◽  
Real Time ◽  
Near Infrared ◽  
Incidence Rates ◽  
Nerve Sparing ◽  
Voltage Sensitive Dye ◽  
Cavernous Nerve ◽  
Nerve Network ◽  
Stimulation Of

AbstractDespite current progress achieved in the surgical technique of radical prostatectomy, post-operative complications such as erectile dysfunction and urinary incontinence persist at high incidence rates. In this paper, we present a methodology for functional intra-operative localization of the cavernous nerve (CN) network for nerve-sparing radical prostatectomy using near-infrared cyanine voltage-sensitive dye (VSD) imaging, which visualizes membrane potential variations in the CN and its branches (CNB) in real time. As a proof-of-concept experiment, we demonstrate a functioning complex nerve network in response to electrical stimulation of the CN, which was clearly differentiated from surrounding tissues in an in vivo rat prostate model. Stimulation of an erection was confirmed by correlative intracavernosal pressure (ICP) monitoring. Within 10 minutes, we performed trans-fascial staining of the CN by direct VSD administration. Our findings suggest the applicability of VSD imaging for real-time, functional imaging guidance during nerve-sparing radical prostatectomy.

scholarly journals Nerve-sparing surgery in current gynecologic oncology

2021 ◽  
Vol 19 (1) ◽  
pp. e27-e32
Author(s):  
Paweł Gruszecki ◽  
◽  
Kazimierz Pityński ◽  
Keyword(s):  
Quality Of Life ◽  
Surgical Procedures ◽  
Radical Hysterectomy ◽  
Gynecologic Oncology ◽  
Pelvic Lymphadenectomy ◽  
Autonomic Nerve ◽  
Operation Technique ◽  
Nerve Sparing ◽  
Modern Approach

Nerve-sparing surgery is currently a very important topic in gynecologic oncology. In this review, it is shown that radical hysterectomy is not the only operation where the nerve-sparing technique can be used. Most surgical procedures in modern gynecologic oncology should spare the autonomic nerve structures. The review includes recently published articles precisely describing the nerve-sparing techniques in paraaortic and pelvic lymphadenectomy, and the modern approach to radical nerve-sparing hysterectomy. It has been shown in the literature that the quality of life of patients is directly dependent on the operation technique and its extension. As mentioned above, the nerve-sparing technique needs to be used not only in surgical procedures for cervical cancer, but more extensively also for endometrial and ovarian cancers. Modern techniques demonstrate that such an operation can be suitable both for the radicality and improved quality of life. Results of such operations are comparable to the old – not nerve-sparing techniques – both in terms of progression-free survival and overall survival. Nerve-sparing surgery in gynecologic oncology is our future. Better quality of life and greater patient satisfaction should be our goals. Studies are needed for better examination and comparison of the presented systematic nerve-sparing operations of lymphadenectomy in ovarian and endometrial cancers, and also combined with nerve-sparing radical hysterectomy.

Construction of a near-infrared responsive upconversion nanoplatform against hypoxic tumors via NO-enhanced photodynamic therapy

Nanoscale ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 7875-7887 ◽  
Author(s):  
Ying Lan ◽  
Xiaohui Zhu ◽  
Ming Tang ◽  
Yihan Wu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Upconversion Nanoparticles

A near-infrared (NIR) activated theranostic nanoplatform based on upconversion nanoparticles (UCNPs) is developed in order to overcome the hypoxia-associated resistance in photodynamic therapy by photo-release of NO upon NIR illumination.

A nitroreductase-activatable near-infrared theranostic photosensitizer for photodynamic therapy under mild hypoxia

2020 ◽  
Vol 56 (43) ◽  
pp. 5819-5822
Author(s):  
Jing Zheng ◽  
Yongzhuo Liu ◽  
Fengling Song ◽  
Long Jiao ◽  
Yingnan Wu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Triplet State ◽  
Near Infrared ◽  
Mild Hypoxia

In this study, a near-infrared (NIR) theranostic photosensitizer was developed based on a heptamethine aminocyanine dye with a long-lived triplet state.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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