Needle-size lensless fiber endoscope for optical stretching and 3D imaging with cellular resolution (Conference Presentation)

2020 ◽  
Author(s):  
Elias Scharf ◽  
Robert Kuschmierz ◽  
Jürgen W. Czarske
Keyword(s):  
3D Imaging ◽  
Needle Size ◽  
Cellular Resolution ◽  
Optical Stretching

scholarly journals Specification of oxytocinergic and vasopressinergic circuits in the developing mouse brain

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
María del Pilar Madrigal ◽  
Sandra Jurado
Keyword(s):  
Autism Spectrum Disorder ◽  
Mouse Brain ◽  
Arginine Vasopressin ◽  
3D Imaging ◽  
Autism Spectrum ◽  
Spectrum Disorder ◽  
Cellular Resolution ◽  
Developing Mouse Brain ◽  
Developmental Dynamics ◽  
Appropriate Social Behaviors

AbstractOxytocin (OXT) and arginine vasopressin (AVP) support a broad range of behaviors and homeostatic functions including sex-specific and context-appropriate social behaviors. Although the alterations of these systems have been linked with social-related disorders such as autism spectrum disorder, their formation and developmental dynamics remain largely unknown. Using novel brain clearing techniques and 3D imaging, we have reconstructed the specification of oxytocinergic and vasopressinergic circuits in the developing mouse brain with unprecedented cellular resolution. A systematic quantification indicates that OXT and AVP neurons in the hypothalamus display distinctive developmental dynamics and high cellular plasticity from embryonic to early postnatal stages. Our findings reveal new insights into the specification and consolidation of neuropeptidergic systems in the developing CNS.

scholarly journals A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Gail McConnell ◽  
Johanna Trägårdh ◽  
Rumelo Amor ◽  
John Dempster ◽  
Es Reid ◽  
...  
Keyword(s):  
3D Imaging ◽  
Numerical Aperture ◽  
Depth Resolution ◽  
Optical Microscope ◽  
Mouse Embryos ◽  
Lens System ◽  
Optical Lens ◽  
Cellular Resolution ◽  
Human Genes ◽  
Confocal Images

Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal and nonlinear microscopy. This is a serious limitation in important areas, including the phenotypic screening of human genes in transgenic mice by study of embryos undergoing advanced organogenesis. We have built an optical lens system for 3D imaging of objects up to 6 mm wide and 3 mm thick with depth resolution of only a few microns instead of the tens of microns currently attained, allowing sub-cellular detail to be resolved throughout the volume. We present this lens, called the Mesolens, with performance data and images from biological specimens including confocal images of whole fixed and intact fluorescently-stained 12.5-day old mouse embryos.

scholarly journals Specification of oxytocinergic and vasopressinergic circuits in the developing mouse brain

2020 ◽  
Author(s):  
M. Pilar Madrigal Verdú ◽  
Sandra Jurado
Keyword(s):  
Mouse Brain ◽  
Arginine Vasopressin ◽  
3D Imaging ◽  
Common Features ◽  
Critical Step ◽  
Cellular Resolution ◽  
Developing Mouse Brain ◽  
And Function ◽  
Developmental Dynamics ◽  
Appropriate Social Behaviors

AbstractOxytocin (OXT) and arginine vasopressin (AVP) support sex-specific and context-appropriate social behaviors. Although alterations of these systems underlie the appearance of neuropsychiatric disorders, their formation and developmental dynamics remain largely unknown. Using novel brain clearing techniques and 3D imaging, we have reconstructed the specification of oxytocinergic and vasopressinergic circuits in the developing mouse brain with unprecedented cellular resolution. A systematic quantification indicates that OXT and AVP neurons in the hypothalamus display distinctive dynamics, but also share common features as a high cellular plasticity from embryonic to early postnatal stages. Our findings reveal new insights into the appearance and consolidation of neuropeptidergic systems in the developing CNS which is a critical step to unveil brain formation and function.

Design and calibration of an IVEM for general 3-dimensional imaging of non-diffracting materials

1992 ◽  
Vol 50 (2) ◽  
pp. 1040-1041
Author(s):  
Neil Rowlands ◽  
Jeff Price ◽  
Michael Kersker ◽  
Seichi Suzuki ◽  
Steve Young ◽  
...  
Keyword(s):  
3D Imaging ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
3 Dimensional ◽  
3D Microstructure ◽  
Image Translation ◽  
Digital Correlation ◽  
On Line ◽  
Mechanical Stage ◽  
Projection Angle

Three-dimensional (3D) microstructure visualization on the electron microscope requires that the sample be tilted to different positions to collect a series of projections. This tilting should be performed rapidly for on-line stereo viewing and precisely for off-line tomographic reconstruction. Usually a projection series is collected using mechanical stage tilt alone. The stereo pairs must be viewed off-line and the 60 to 120 tomographic projections must be aligned with fiduciary markers or digital correlation methods. The delay in viewing stereo pairs and the alignment problems in tomographic reconstruction could be eliminated or improved by tilting the beam if such tilt could be accomplished without image translation.A microscope capable of beam tilt with simultaneous image shift to eliminate tilt-induced translation has been investigated for 3D imaging of thick (1 μm) biologic specimens. By tilting the beam above and through the specimen and bringing it back below the specimen, a brightfield image with a projection angle corresponding to the beam tilt angle can be recorded (Fig. 1a).

3D imaging of the whole Spine at 3 Tesla, initial experiences

2006 ◽  
Vol 177 (01) ◽  
Author(s):  
EA Rodegerdts ◽  
A Boss ◽  
K Riemarzik ◽  
M Lichy ◽  
F Schick ◽  
...  
Keyword(s):  
3D Imaging ◽  
3 Tesla ◽  
Whole Spine

From the Inside Out: Practical Application of 3D Imaging Techniques in Art Conservation

2018 ◽  
Vol 2018 (1) ◽  
pp. 151-156
Author(s):  
Scott Geffert ◽  
Daniel Hausdorf ◽  
Joseph Coscia ◽  
Oi-Cheong Lee ◽  
Dahee Han ◽  
...  
Keyword(s):  
3D Imaging ◽  
Imaging Techniques ◽  
Art Conservation ◽  
Practical Application ◽  
Inside Out ◽  
3D Imaging Techniques
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