High-throughput 3D imaging of multiple macro-scale organs at cellular resolution by compressed-sensing light-sheet microscopy

2021 ◽  
Author(s):  
Fang Zhao ◽  
Wenyang Feng ◽  
Chunyu Fang ◽  
Yao Zhou ◽  
Peng Fei
Keyword(s):  
Compressed Sensing ◽  
High Throughput ◽  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Cellular Resolution ◽  
Macro Scale

scholarly journals Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chunyu Fang ◽  
Tingting Yu ◽  
Tingting Chu ◽  
Wenyang Feng ◽  
Fang Zhao ◽  
...  
Keyword(s):  
Compressed Sensing ◽  
Light Sheet ◽  
System Level ◽  
Projection Neurons ◽  
Long Distance ◽  
Rapid Acquisition ◽  
Light Sheet Microscopy ◽  
Macro Scale ◽  
Subcellular Resolution ◽  
Content Aware

AbstractRapid 3D imaging of entire organs and organisms at cellular resolution is a recurring challenge in life science. Here we report on a computational light-sheet microscopy able to achieve minute-timescale high-resolution mapping of entire macro-scale organs. Through combining a dual-side confocally-scanned Bessel light-sheet illumination which provides thinner-and-wider optical sectioning of deep tissues, with a content-aware compressed sensing (CACS) computation pipeline which further improves the contrast and resolution based on a single acquisition, our approach yields 3D images with high, isotropic spatial resolution and rapid acquisition over two-order-of-magnitude faster than conventional 3D microscopy implementations. We demonstrate the imaging of whole brain (~400 mm3), entire gastrocnemius and tibialis muscles (~200 mm3) of mouse at ultra-high throughput of 5~10 min per sample and post-improved subcellular resolution of ~ 1.5 μm (0.5-μm iso-voxel size). Various system-level cellular analyses, such as mapping cell populations at different brain sub-regions, tracing long-distance projection neurons over the entire brain, and calculating neuromuscular junction occupancy across whole muscle, are also readily accomplished by our method.

scholarly journals Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy

2019 ◽  
Author(s):  
Chunyu Fang ◽  
Tingting Chu ◽  
Tingting Yu ◽  
Yujie Huang ◽  
Yusha Li ◽  
...  
Keyword(s):  
Compressed Sensing ◽  
Spatial Resolution ◽  
Light Sheet ◽  
System Level ◽  
Projection Neurons ◽  
Long Distance ◽  
Light Sheet Microscopy ◽  
Macro Scale ◽  
Subcellular Resolution ◽  
Content Aware

AbstractInstant 3D imaging of entire organs and organisms at cellular resolution is a recurring challenge in life science. Here we report on a computational light-sheet microscopy able to achieve minute-timescale mapping of entire macro-scale organs at high spatial resolution, thereby overcoming the throughput limit of current 3D microscopy implementations. Through combining a dual-side confocally-scanned Bessel light-sheet illumination which provides thinner-and-wider optical sectioning of deep tissues, with a content-aware compressed sensing (CACS) computation pipeline which further improves the contrast and resolution based on a single acquisition, our method yields 3D images with high, isotropic spatial resolution and rapid acquisition improved by two-orders of magnitude. We demonstrate the imaging of whole brain (∼400 mm3), entire gastrocnemius and tibialis muscles (∼200 mm3) of mouse at subcellular resolution (0.5-μm isovoxel) and ultra-high throughput of 5∼10 minutes per sample. Various system-level cellular analyses, such as mapping cell populations at different brain sub-regions, tracing long-distance projection neurons over the entire brain, and calculating neuromuscular junction occupancy across whole muscle, were also readily enabled by our method.

scholarly journals High-throughput live imaging using Light Sheet Microscopy

2020 ◽  
Author(s):  
Emilio J. Gualda ◽  
Matteo Bernardello ◽  
Maria Marsal ◽  
Pablo Loza Alvarez
Keyword(s):  
High Throughput ◽  
Light Sheet ◽  
Live Imaging ◽  
Light Sheet Microscopy

Rapid high resolution 3D imaging of expanded biological specimens with lattice light sheet microscopy

Methods ◽  
2020 ◽  
Vol 174 ◽  
pp. 11-19 ◽  
Author(s):  
Yun-Chi Tsai ◽  
Wei-Chun Tang ◽  
Christine Siok Lan Low ◽  
Yen-Ting Liu ◽  
Jyun-Sian Wu ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Quantitative neuroanatomy of all Purkinje cells with light sheet microscopy and high-throughput image analysis

2015 ◽  
Vol 9 ◽  
Author(s):  
Ludovico Silvestri ◽  
Marco Paciscopi ◽  
Paolo Soda ◽  
Filippo Biamonte ◽  
Giulio Iannello ◽  
...  
Keyword(s):  
Image Analysis ◽  
Purkinje Cells ◽  
High Throughput ◽  
Light Sheet ◽  
Light Sheet Microscopy

3D imaging of cleared human skin biopsies using light-sheet microscopy: A new way to visualize in-depth skin structure

2018 ◽  
Vol 24 (2) ◽  
pp. 294-303 ◽  
Author(s):  
S. Abadie ◽  
C. Jardet ◽  
J. Colombelli ◽  
B. Chaput ◽  
A. David ◽  
...  
Keyword(s):  
Human Skin ◽  
3D Imaging ◽  
Light Sheet ◽  
Skin Structure ◽  
Light Sheet Microscopy ◽  
Skin Biopsies

scholarly journals 3D Imaging of BABB Cleared Whole-Mount Organs Using Light Sheet Microscopy

2018 ◽  
Vol 24 (S1) ◽  
pp. 1390-1391
Author(s):  
Kingsley A. Boateng ◽  
Austin Cyphersmith ◽  
Glenn A. Fried ◽  
Barghav S. Sivaguru ◽  
Xiaochen Lu ◽  
...  
Keyword(s):  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Whole Mount

scholarly journals A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

2013 ◽  
Vol 21 (12) ◽  
pp. 14474 ◽  
Author(s):  
Jianglai Wu ◽  
Jianping Li ◽  
Robert K.Y. Chan
Keyword(s):  
High Throughput ◽  
3D Imaging ◽  
Light Sheet ◽  
Flow Cytometer

scholarly journals A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues

2021 ◽  
Author(s):  
Adam Glaser ◽  
Kevin Bishop ◽  
Lindsey Barner ◽  
Etsuo Susaki ◽  
Shimpei Kubota ◽  
...  
Keyword(s):  
Hybrid System ◽  
High Throughput ◽  
Light Sheet ◽  
Refractive Indices ◽  
Volumetric Imaging ◽  
Tissue Clearing ◽  
Sample Geometry ◽  
Multi Scale ◽  
Light Sheet Microscopy ◽  
User Friendly

Abstract Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address imaging applications with varied requirements in terms of resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), and compatibility with tissue-clearing protocols and sample holders of various refractive indices. We present a ‘hybrid’ system that combines a novel non-orthogonal dual-objective and conventional (orthogonal) open-top light-sheet architecture for versatile multi-scale volumetric imaging.

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