Stimulated Raman imaging with chemical probes for subcellular bioanalysis (Conference Presentation)

2020 ◽  
Author(s):  
Lu Wei ◽  
Kun Miao ◽  
Jiajun Du
Keyword(s):  
Raman Imaging ◽  
Chemical Probes ◽  
Stimulated Raman

Stimulated Raman Imaging Reveals Aberrant Lipogenesis as a Metabolic Marker for Azole-ResistantCandida albicans

2017 ◽  
Vol 89 (18) ◽  
pp. 9822-9829 ◽  
Author(s):  
Caroline W. Karanja ◽  
Weili Hong ◽  
Waleed Younis ◽  
Hassan E. Eldesouky ◽  
Mohamed N. Seleem ◽  
...  
Keyword(s):  
Raman Imaging ◽  
Metabolic Marker ◽  
Stimulated Raman

Volumetric stimulated Raman imaging with a high-speed deformable mirror (Conference Presentation)

2019 ◽  
Author(s):  
Peng Lin ◽  
Hongli Ni ◽  
Huate Li ◽  
Fengyuan Deng ◽  
Nicholas A. Vickers ◽  
...  
Keyword(s):  
High Speed ◽  
Raman Imaging ◽  
Deformable Mirror ◽  
Stimulated Raman

Widely tunable, fully automated, all-fiber dual-color laser system for stimulated Raman imaging

2017 ◽  
Author(s):  
Thomas Gottschall ◽  
Tobias Meyer ◽  
Cesar Jauregui ◽  
Florian Just ◽  
Tino Eidam ◽  
...  
Keyword(s):  
Laser System ◽  
Raman Imaging ◽  
Dual Color ◽  
Stimulated Raman

scholarly journals Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199695 ◽  
Author(s):  
Asefeh Golreihan ◽  
Christian Steuwe ◽  
Lineke Woelders ◽  
Arne Deprez ◽  
Yasuhiko Fujita ◽  
...  
Keyword(s):  
State Assessment ◽  
Raman Imaging ◽  
Label Free ◽  
Stimulated Raman

scholarly journals Stimulated Raman histology in the neurosurgical workflow of a major European neurosurgical center — part A

2021 ◽  
Author(s):  
Nicolas Neidert ◽  
Jakob Straehle ◽  
Daniel Erny ◽  
Vlad Sacalean ◽  
Amir El Rahal ◽  
...  
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Tumor Resection ◽  
Raman Imaging ◽  
Histopathological Analysis ◽  
Histopathological Diagnosis ◽  
High Quality ◽  
Safety Control ◽  
Quality Control Tool ◽  
Stimulated Raman

AbstractHistopathological diagnosis is the current standard for the classification of brain and spine tumors. Raman spectroscopy has been reported to allow fast and easy intraoperative tissue analysis. Here, we report data on the intraoperative implementation of a stimulated Raman histology (SRH) as an innovative strategy offering intraoperative near real-time histopathological analysis. A total of 429 SRH images from 108 patients were generated and analyzed by using a Raman imaging system (Invenio Imaging Inc.). We aimed at establishing a dedicated workflow for SRH serving as an intraoperative diagnostic, research, and quality control tool in the neurosurgical operating room (OR). First experiences with this novel imaging modality were reported and analyzed suggesting process optimization regarding tissue collection, preparation, and imaging. The Raman imaging system was rapidly integrated into the surgical workflow of a large neurosurgical center. Within a few minutes of connecting the device, the first high-quality images could be acquired in a “plug-and-play” manner. We did not encounter relevant obstacles and the learning curve was steep. However, certain prerequisites regarding quality and acquisition of tissue samples, data processing and interpretation, and high throughput adaptions must be considered. Intraoperative SRH can easily be integrated into the workflow of neurosurgical tumor resection. Considering few process optimizations that can be implemented rapidly, high-quality images can be obtained near real time. Hence, we propose SRH as a complementary tool for the diagnosis of tumor entity, analysis of tumor infiltration zones, online quality and safety control and as a research tool in the neurosurgical OR.

Stimulated Raman Imaging of Lipids Droplets

2018 ◽  
Author(s):  
Maria Antonietta Ferrara ◽  
Angela Filograna ◽  
Annalisa D'Arco ◽  
Rajeev Ranjan ◽  
Carmen Valente ◽  
...  
Keyword(s):  
Raman Imaging ◽  
Stimulated Raman
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