High-sensitivity stimulated Raman imaging with chemical tags (Conference Presentation)

2020 ◽  
Author(s):  
Lu Wei
Keyword(s):  
High Sensitivity ◽  
Raman Imaging ◽  
Chemical Tags ◽  
Stimulated Raman

High‐sensitivity Raman imaging of the surface of casted glass plates

2021 ◽  
Author(s):  
M. Jacquemin ◽  
P. Simon ◽  
A. Canizarès ◽  
L. Hennet ◽  
C. Bessada ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Raman Imaging ◽  
Glass Plates

Stimulated Raman Imaging Reveals Aberrant Lipogenesis as a Metabolic Marker for Azole-ResistantCandida albicans

2017 ◽  
Vol 89 (18) ◽  
pp. 9822-9829 ◽  
Author(s):  
Caroline W. Karanja ◽  
Weili Hong ◽  
Waleed Younis ◽  
Hassan E. Eldesouky ◽  
Mohamed N. Seleem ◽  
...  
Keyword(s):  
Raman Imaging ◽  
Metabolic Marker ◽  
Stimulated Raman

Volumetric stimulated Raman imaging with a high-speed deformable mirror (Conference Presentation)

2019 ◽  
Author(s):  
Peng Lin ◽  
Hongli Ni ◽  
Huate Li ◽  
Fengyuan Deng ◽  
Nicholas A. Vickers ◽  
...  
Keyword(s):  
High Speed ◽  
Raman Imaging ◽  
Deformable Mirror ◽  
Stimulated Raman

Widely tunable, fully automated, all-fiber dual-color laser system for stimulated Raman imaging

2017 ◽  
Author(s):  
Thomas Gottschall ◽  
Tobias Meyer ◽  
Cesar Jauregui ◽  
Florian Just ◽  
Tino Eidam ◽  
...  
Keyword(s):  
Laser System ◽  
Raman Imaging ◽  
Dual Color ◽  
Stimulated Raman

scholarly journals Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199695 ◽  
Author(s):  
Asefeh Golreihan ◽  
Christian Steuwe ◽  
Lineke Woelders ◽  
Arne Deprez ◽  
Yasuhiko Fujita ◽  
...  
Keyword(s):  
State Assessment ◽  
Raman Imaging ◽  
Label Free ◽  
Stimulated Raman

scholarly journals Super-resolution vibrational microscopy by stimulated Raman excited fluorescence

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Hanqing Xiong ◽  
Naixin Qian ◽  
Yupeng Miao ◽  
Zhilun Zhao ◽  
Chen Chen ◽  
...  
Keyword(s):  
Laser Excitation ◽  
Signal To Noise Ratio ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
High Sensitivity ◽  
Excitation Power ◽  
Stimulated Emission ◽  
Imaging Method ◽  
High Contrast ◽  
Stimulated Raman

AbstractInspired by the revolutionary impact of super-resolution fluorescence microscopy, super-resolution Raman imaging has been long pursued because of its much higher chemical specificity than the fluorescence counterpart. However, vibrational contrasts are intrinsically less sensitive compared with fluorescence, resulting in only mild resolution enhancement beyond the diffraction limit even with strong laser excitation power. As such, it is still a great challenge to achieve biocompatible super-resolution vibrational imaging in the optical far-field. In 2019 Stimulated Raman Excited Fluorescence (SREF) was discovered as an ultrasensitive vibrational spectroscopy that combines the high chemical specificity of Raman scattering and the superb sensitivity of fluorescence detection. Herein we developed a novel super-resolution vibrational imaging method by harnessing SREF as the contrast mechanism. We first identified the undesired role of anti-Stokes fluorescence background in preventing direct adoption of super-resolution fluorescence technique. We then devised a frequency-modulation (FM) strategy to remove the broadband backgrounds and achieved high-contrast SREF imaging. Assisted by newly synthesized SREF dyes, we realized multicolor FM-SREF imaging with nanometer spectral resolution. Finally, by integrating stimulated emission depletion (STED) with background-free FM-SREF, we accomplished high-contrast super-resolution vibrational imaging with STED-FM-SREF whose spatial resolution is only determined by the signal-to-noise ratio. In our proof-of-principle demonstration, more than two times of resolution improvement is achieved in biological systems with moderate laser excitation power, which shall be further refined with optimized instrumentation and imaging probes. With its super resolution, high sensitivity, vibrational contrast, and mild laser excitation power, STED-FM-SREF microscopy is envisioned to aid a wide variety of applications.

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