3D reconstruction of lipid droplets in single cells by stimulated raman imaging

2018 ◽  
Author(s):  
M.A. Ferrara ◽  
R. Ranjan ◽  
A. Filograna ◽  
A. D'Arco ◽  
C. Valente ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Lipid Droplets ◽  
Single Cells ◽  
Raman Imaging ◽  
Stimulated Raman

scholarly journals Novel strategies of Raman imaging for monitoring intracellular retinoid metabolism in cancer cells

2020 ◽  
Author(s):  
Halina Abramczyk ◽  
Anna Imiela ◽  
Jakub Surmacki
Keyword(s):  
Cancer Cells ◽  
Conformational Changes ◽  
Lipid Droplets ◽  
Binding Proteins ◽  
Single Cells ◽  
Raman Imaging ◽  
Label Free ◽  
Aberrant Expression ◽  
Retinoid Metabolism ◽  
Biochemical Imaging

AbstractWe developed a label-free Raman method for whole-cell biochemical imaging to detect molecular processes that occur in normal and cancer brain cells due to retinol transport in human cancers at the level of isolated organelles. Our approach allows the creation of biochemical maps of lipid droplets, mitochondria and nuclei in single cells. The maps were capable of discriminating triglycerides (TAG) from retinyl ester (RE) in lipid droplets (LD), providing an excellent tool to monitor intracellular retinoid metabolism. We detected spectral changes that arose in proteins and lipids due to retinoid metabolism in human cell lines of normal astrocytes and high-grade cancer cells of glioblastoma as well as in human medulloblastoma and glioblastoma tissue. Raman imaging is an effective tool for monitoring of retinoids and retinol binding proteins involved in carcinogenesis, as monitored by the unique spectral signatures of vibrations. We found two functionally distinct lipid droplets: TAG-LD, for energy storage, and RE-LD, for regulating the level of apo-CRBP1 in cytosol. Raman polarization measurements revealed the occurrence of conformational changes affecting discrete regions of proteins associated with retinol binding. Aberrant expression of retinoids and retinol binding proteins in human tumours were localized in lipid droplets, mitochondria and nuclei according Raman imaging.

Stimulated Raman Imaging Reveals Aberrant Lipogenesis as a Metabolic Marker for Azole-ResistantCandida albicans

2017 ◽  
Vol 89 (18) ◽  
pp. 9822-9829 ◽  
Author(s):  
Caroline W. Karanja ◽  
Weili Hong ◽  
Waleed Younis ◽  
Hassan E. Eldesouky ◽  
Mohamed N. Seleem ◽  
...  
Keyword(s):  
Raman Imaging ◽  
Metabolic Marker ◽  
Stimulated Raman

Cell Nuclei and Lipid Droplets Quantification in Stimulated Raman Images

2021 ◽  
Author(s):  
Adiel Felsen ◽  
Yuhao Yuan ◽  
Nikolas Burzynski ◽  
David Reitano ◽  
Zhibo Wang ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Cell Nuclei ◽  
Stimulated Raman

Estimation of the content of lipids composing endothelial lipid droplets based on Raman imaging

2020 ◽  
Vol 1865 (9) ◽  
pp. 158758 ◽  
Author(s):  
Marta Z. Pacia ◽  
Katarzyna Majzner ◽  
Krzysztof Czamara ◽  
Magdalena Sternak ◽  
Stefan Chlopicki ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Raman Imaging

Monitoring UVR induced damage in single cells and isolated nuclei using SR-FTIR microspectroscopy and 3D confocal Raman imaging

The Analyst ◽  
2014 ◽  
Vol 139 (17) ◽  
pp. 4200-4209 ◽  
Author(s):  
Ewelina Lipiec ◽  
Keith R. Bambery ◽  
Philip Heraud ◽  
Wojciech M. Kwiatek ◽  
Don McNaughton ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Lipid Accumulation ◽  
Single Cells ◽  
Raman Imaging ◽  
Isolated Nuclei ◽  
Ftir Microspectroscopy ◽  
Confocal Raman ◽  
Ftir And Raman Spectroscopy

Melanocytes exposed to artificial sunlight and analysed with FTIR and Raman spectroscopy show changes in DNA bands and evidence of lipid accumulation.

Volumetric stimulated Raman imaging with a high-speed deformable mirror (Conference Presentation)

2019 ◽  
Author(s):  
Peng Lin ◽  
Hongli Ni ◽  
Huate Li ◽  
Fengyuan Deng ◽  
Nicholas A. Vickers ◽  
...  
Keyword(s):  
High Speed ◽  
Raman Imaging ◽  
Deformable Mirror ◽  
Stimulated Raman

scholarly journals Quantitative imaging of lipid droplets in single cells

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 753-765 ◽  
Author(s):  
Anushka Gupta ◽  
Gabriel F. Dorlhiac ◽  
Aaron M. Streets
Keyword(s):  
Lipid Droplets ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Cell Level ◽  
Spatial Characterization ◽  
Coherent Raman Scattering ◽  
Coherent Raman ◽  
Computational Image Analysis ◽  
Non Destructive

Non-destructive spatial characterization of lipid droplets using coherent Raman scattering microscopy and computational image analysis algorithms at the single-cell level.

scholarly journals Zinc Phthalocyanine Photochemistry by Raman Imaging, Fluorescence Spectroscopy and Femtosecond Spectroscopy in Normal and Cancerous Human Colon Tissues and Single Cells

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2688 ◽  
Author(s):  
Beata Brozek-Pluska ◽  
Arkadiusz Jarota ◽  
Rafal Kania ◽  
Halina Abramczyk
Keyword(s):  
Single Cells ◽  
Blue Shift ◽  
Human Colon ◽  
Raman Imaging ◽  
Zinc Phthalocyanine ◽  
Label Free ◽  
Colon Tissue ◽  
Tissue Samples ◽  
Colon Cells ◽  
Normal Human

Photodynamic therapy is a clinically approved alternative method for cancer treatment in which a combination of nontoxic drugs known as photosensitizers and oxygen is used. Despite intensive investigations and encouraging results, zinc phthalocyanines (ZnPcs) have not yet been approved as photosensitizers for clinical use. Label-free Raman imaging of nonfixed and unstained normal and cancerous colon human tissues and normal human CCD18-Co and cancerous CaCo-2 cell lines, without and after adding ZnPcS4 photosensitizer, was analyzed. The biochemical composition of normal and cancerous colon tissues and colon cells without and after adding ZnPcS4 at the subcellular level was determined. Analyzing the fluorescence/Raman signals of ZnPcS4, we found that in normal human colon tissue samples, in contrast to cancerous ones, there is a lower affinity to ZnPcS4 phthalocyanine. Moreover, a higher concentration in cancerous tissue was concomitant with a blue shift of the maximum peak position specific for the photosensitizer from 691–695 nm to 689 nm. Simultaneously for both types of samples, the signal was observed in the monomer region, confirming the excellent properties of ZnPcS4 for photo therapy (PDT). For colon cell experiments with a lower concentration of ZnPcS4 photosensitizer, c = 1 × 10−6 M, the phthalocyanine was localized in mitochondria/lipid structures; for a higher concentration, c = 9 × 10−6 M, localization inside the nucleus was predominant. Based on time-resolved experiments, we found that ZnPcS4 in the presence of biological interfaces features longer excited-state lifetime photosensitizers compared to the aqueous solution and bare ZnPcS4 film on CaF2 substrate, which is beneficial for application in PDT.

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