Nanofluidic label-free single biomolecule detection (Conference Presentation)

2020 ◽  
Author(s):  
Barbora Spackova ◽  
Johan Tenghamn ◽  
Joachim Fritzsche ◽  
Christoph Langhammer
Keyword(s):  
Label Free ◽  
Biomolecule Detection

Phage-based label-free biomolecule detection in an opto-fluidic ring resonator

2008 ◽  
Vol 24 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Hongying Zhu ◽  
Ian M. White ◽  
Jonathan D. Suter ◽  
Xudong Fan
Keyword(s):  
Ring Resonator ◽  
Label Free ◽  
Biomolecule Detection

Improved Sensitivity of Dielectric Modulated Junctionless Transistor for Nanoscale Biosensor Design

2020 ◽  
Vol 18 (4) ◽  
pp. 328-333
Author(s):  
Shradhya Singh ◽  
Shashi Bala ◽  
Balwant Raj ◽  
Balwinder Raj
Keyword(s):  
Threshold Voltage ◽  
Drain Current ◽  
Label Free ◽  
Asymmetric Mode ◽  
Biomolecule Detection ◽  
Gate Operation ◽  
Voltage Change ◽  
Mode Of Operation ◽  
Junctionless Transistor ◽  
Detection Of Biomolecules

This work has proposed a device i.e., Dielectric Modulated (DM) Junctionless Transistor which is utilizes as Label-Free (LF) electrical characteristic detection of bio-molecules. The electrical characteristics used for the detection of biomolecules are electric field, surface potential, drain current and threshold voltage (Vth). Due to immobilization of biomolecules in the cavity region, the threshold voltage change in comparison to the absence of biomolecule, which is utilizes as the sensitivity metric. The sensitivity of biomolecule detection can be enhanced by using asymmetric gate operation of the device. In asymmetric mode the degree of sensitivity is almost five times higher than that of the symmetric mode of operation.

Label-Free Biomolecule Detection in Physiological Solutions With Enhanced Sensitivity Using Graphene Nanogrids FET Biosensor

2018 ◽  
Vol 17 (4) ◽  
pp. 433-442 ◽  
Author(s):  
R. Ray ◽  
J. Basu ◽  
W. A. Gazi ◽  
N. Samanta ◽  
K. Bhattacharyya ◽  
...  
Keyword(s):  
Label Free ◽  
Biomolecule Detection ◽  
Enhanced Sensitivity

Phage-based opto-fluidic ring resonator for label-free biomolecule detection

2008 ◽  
Author(s):  
Hongying Zhu ◽  
Ian M. White ◽  
Jonathan D. Suter ◽  
Xudong Fan
Keyword(s):  
Ring Resonator ◽  
Label Free ◽  
Biomolecule Detection

A novel label-free biosensor based on self-assembled aptamer/GO architecture for sensitive detection of biomolecules

2015 ◽  
Vol 7 (13) ◽  
pp. 5606-5610 ◽  
Author(s):  
Zhimei Huang ◽  
Jia Ge ◽  
Lan Liu ◽  
Jianhui Jiang ◽  
Guoli Shen ◽  
...  
Keyword(s):  
Conformational Change ◽  
Thioflavin T ◽  
Sensitive Detection ◽  
Label Free ◽  
Biomolecule Detection ◽  
Self Assembled ◽  
Detection Of Biomolecules

We developed a novel label-free biosensor for biomolecule detection based on the thioflavin T (ThT)-induced conformational change of guanine-rich oligonucleotides and self-assembled aptamer/GO nanosheet architecture.

Development of LC resonator for label-free biomolecule detection

2008 ◽  
Vol 143 (2) ◽  
pp. 279-285 ◽  
Author(s):  
Young-Il Kim ◽  
Yunkwon Park ◽  
Hong Koo Baik
Keyword(s):  
Label Free ◽  
Biomolecule Detection ◽  
Lc Resonator

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

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