Label-Free and Ultrasensitive Biomolecule Detection Based on Aggregation Induced Emission Fluorogen via Target-Triggered Hemin/G-Quadruplex-Catalyzed Oxidation Reaction

2018 ◽  
Vol 10 (5) ◽  
pp. 4561-4568 ◽  
Author(s):  
Haiyin Li ◽  
Jiafu Chang ◽  
Panpan Gai ◽  
Feng Li
Keyword(s):  
Oxidation Reaction ◽  
Label Free ◽  
Biomolecule Detection ◽  
Aggregation Induced Emission ◽  
G Quadruplex ◽  
Catalyzed Oxidation

Visual observation of G-quadruplex DNA with the label-free fluorescent probe silole with aggregation-induced emission

2009 ◽  
Vol 17 (22) ◽  
pp. 7743-7748 ◽  
Author(s):  
Jing Huang ◽  
Ming Wang ◽  
Yangyang Zhou ◽  
Xiaocheng Weng ◽  
Liang Shuai ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Visual Observation ◽  
Label Free ◽  
Quadruplex Dna ◽  
Aggregation Induced Emission ◽  
G Quadruplex

A novel label-free optical cysteine sensor based on the competitive oxidation reaction catalyzed by G-quadruplex halves

2013 ◽  
Vol 43 ◽  
pp. 268-273 ◽  
Author(s):  
Haichao Su ◽  
Fengmin Qiao ◽  
Ruihuan Duan ◽  
Lijian Chen ◽  
Shiyun Ai
Keyword(s):  
Oxidation Reaction ◽  
Label Free ◽  
G Quadruplex

A label-free fluorescent aptasensor based on HCR and G-quadruplex DNAzymes for the detection of prostate-specific antigen

The Analyst ◽  
2021 ◽  
Author(s):  
Ruirui Zhao ◽  
Lu Zhao ◽  
Haidi Feng ◽  
Xiaoliang Chen ◽  
Huilin Zhang ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sensing Platforms ◽  
G Quadruplex ◽  
Fluorescent Aptasensor

Fluorescence sensing platforms based on HCR and G-quadruplex DNAzyme amplification strategies for the detection of prostate-specific antigen.

Capturing Protein Droplets: Label-free Visualization and Detection of Protein Liquid-Liquid Phase Separation with an Aggregation-Induced Emission Fluorogen

2021 ◽  
Author(s):  
Yan-Mei Li ◽  
Chu-Qiao Liang ◽  
Lin Wang ◽  
Yun-Yi Luo ◽  
Qian-Qian Li
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
New Method ◽  
Label Free ◽  
Simple Method ◽  
Aggregation Induced Emission ◽  
Liquid Liquid Phase Separation

We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of...

Phage-based label-free biomolecule detection in an opto-fluidic ring resonator

2008 ◽  
Vol 24 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Hongying Zhu ◽  
Ian M. White ◽  
Jonathan D. Suter ◽  
Xudong Fan
Keyword(s):  
Ring Resonator ◽  
Label Free ◽  
Biomolecule Detection

A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Label Free ◽  
Activity Assay ◽  
G Quadruplex ◽  
Catalytic Hairpin Assembly ◽  
Amplification Strategy

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.

A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme

2018 ◽  
Vol 71 (12) ◽  
pp. 945
Author(s):  
Xin Fu ◽  
He Zhang ◽  
Jie Zhang ◽  
Shi-Tong Wen ◽  
Xing-Cheng Deng
Keyword(s):  
Standard Deviation ◽  
Base Pair ◽  
Limit Of Detection ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Relative Standard ◽  
G Quadruplex ◽  
Catalytically Active ◽  
Partial Cdna

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

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