High throughput optical analysis and sorting of cells and particles in microfluidic systems

2019 ◽  
Author(s):  
Daniel Geiger ◽  
Tobias Neckernuss ◽  
Jonas Pfeil ◽  
Patricia Schwilling ◽  
Othmar Marti
Keyword(s):  
High Throughput ◽  
Optical Analysis ◽  
Microfluidic Systems

scholarly journals The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 297 ◽  
Author(s):  
Kena Song ◽  
Guoqiang Li ◽  
Xiangyang Zu ◽  
Zhe Du ◽  
Liyu Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
New Technologies ◽  
Biomedical Application ◽  
Raw Materials ◽  
Physical Structure ◽  
Microfluidic System ◽  
Microfluidic Chips ◽  
Microfluidic Systems ◽  
Microfluidic Technology

Microfluidic systems have been widely explored based on microfluidic technology, and it has been widely used for biomedical screening. The key parts are the fabrication of the base scaffold, the construction of the matrix environment in the 3D system, and the application mechanism. In recent years, a variety of new materials have emerged, meanwhile, some new technologies have been developed. In this review, we highlight the properties of high throughput and the biomedical application of the microfluidic chip and focus on the recent progress of the fabrication and application mechanism. The emergence of various biocompatible materials has provided more available raw materials for microfluidic chips. The material is not confined to polydimethylsiloxane (PDMS) and the extracellular microenvironment is not limited by a natural matrix. The mechanism is also developed in diverse ways, including its special physical structure and external field effects, such as dielectrophoresis, magnetophoresis, and acoustophoresis. Furthermore, the cell/organ-based microfluidic system provides a new platform for drug screening due to imitating the anatomic and physiologic properties in vivo. Although microfluidic technology is currently mostly in the laboratory stage, it has great potential for commercial applications in the future.

scholarly journals Plastic Microfluidic Systems for High-Throughput Genomic Analysis and Drug Screening

2001 ◽  
Vol 6 (1) ◽  
pp. 71-75
Author(s):  
Maureen T. Cronin ◽  
Travis Boone ◽  
Alexander P. Sassi ◽  
Hongdong Tan ◽  
Qifeng Xue ◽  
...  
Keyword(s):  
High Throughput ◽  
Drug Screening ◽  
Genomic Analysis ◽  
Microfluidic Systems

Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis

2009 ◽  
Vol 81 (12) ◽  
pp. 4813-4821 ◽  
Author(s):  
Linas Mazutis ◽  
Ali Fallah Araghi ◽  
Oliver J. Miller ◽  
Jean-Christophe Baret ◽  
Lucas Frenz ◽  
...  
Keyword(s):  
High Throughput ◽  
Isothermal Amplification ◽  
Microfluidic Systems ◽  
Dna Molecule ◽  
Single Dna Molecule

Label-free high-throughput detection and content sensing of individual droplets in microfluidic systems

Lab on a Chip ◽  
2015 ◽  
Vol 15 (20) ◽  
pp. 4008-4019 ◽  
Author(s):  
Gurkan Yesiloz ◽  
Muhammed Said Boybay ◽  
Carolyn L. Ren
Keyword(s):  
High Throughput ◽  
Integrated Approach ◽  
Label Free ◽  
Microfluidic Systems ◽  
High Throughput Detection ◽  
Individual Droplet

We report a microwave-microfluidics integrated approach capable of detecting droplet at high-throughput and label-free sensing of individual droplet content without physical intrusion.

scholarly journals Index-Matched Microfluidic Cell Array for High throughput Single Cell Optical Analysis

2019 ◽  
Vol 116 (3) ◽  
pp. 446a
Author(s):  
Justin J. Griffin ◽  
Edward R. Polanco ◽  
Thomas A. Zangle
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Optical Analysis ◽  
Cell Array

Impedance spectroscopy-based cell/particle position detection in microfluidic systems

Lab on a Chip ◽  
2017 ◽  
Vol 17 (7) ◽  
pp. 1264-1269 ◽  
Author(s):  
H. Wang ◽  
N. Sobahi ◽  
A. Han
Keyword(s):  
Impedance Spectroscopy ◽  
High Throughput ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Microfluidic Systems ◽  
Microfluidic Platform ◽  
Particle Position ◽  
Position Detection

A high-throughput and low-cost impedance spectroscopy-based microfluidic platform capable of detecting/discriminating the transverse positions of cells/particles flowing within a microfluidic channel.

scholarly journals High-speed particle detection and tracking in microfluidic devices using event-based sensing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 3024-3035
Author(s):  
Jessie Howell ◽  
Tansy C. Hammarton ◽  
Yoann Altmann ◽  
Melanie Jimenez
Keyword(s):  
High Throughput ◽  
High Speed ◽  
Microfluidic Devices ◽  
Microfluidic Systems ◽  
Particle Detection ◽  
Detection And Tracking ◽  
Particle Imaging ◽  
Event Based ◽  
Cost Sensitivity

Event-based sensing offers unique advantages in terms of cost, sensitivity and compatibility with standard microscopes for high-throughput particle imaging in microfluidic systems.

An Ultra-High-Throughput Flow-Focusing Microfluidic Device for Creation of Liquid Droplets in Air

2017 ◽  
Author(s):  
Pooyan Tirandazi ◽  
Gabriel Tomic ◽  
Carlos H. Hidrovo
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
High Speed ◽  
Large Scale ◽  
3D Structure ◽  
Scale Production ◽  
Liquid Droplets ◽  
Flow Focusing ◽  
Microfluidic Systems ◽  
Uniform Array

In this paper a new microfluidic technique is proposed for ultra-high-throughput generation of micron-sized water droplets using a high-speed air. We use a 3D flow-focusing microchannel fabricated in PDMS by multilayer lithography process. The interaction of liquid and gas created three main flow conditions which are: Flooded, Dripping, and Jetting. We characterize the Jetting regime where a capillary jet surrounded by the air breaks up into uniform array of droplets. Frequency of generation and droplet size are reported for the jetting regime under different liquid and gas flows. It was possible to obtain 25μm diameter droplets and much higher frequencies (f≈120 kHz) compared to the state-of-the-art microfluidic systems. We believe the advantages of this platform enables many novel applications such as high-throughput screening of airborne targets and large-scale production of oil-free particles. The 3D structure of this device also eliminates the limitation of the conventional droplet-based microfluidic systems, namely clogging issues due to particle aggregation.

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