scholarly journals High-speed particle detection and tracking in microfluidic devices using event-based sensing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 3024-3035
Author(s):  
Jessie Howell ◽  
Tansy C. Hammarton ◽  
Yoann Altmann ◽  
Melanie Jimenez
Keyword(s):  
High Throughput ◽  
High Speed ◽  
Microfluidic Devices ◽  
Microfluidic Systems ◽  
Particle Detection ◽  
Detection And Tracking ◽  
Particle Imaging ◽  
Event Based ◽  
Cost Sensitivity

Event-based sensing offers unique advantages in terms of cost, sensitivity and compatibility with standard microscopes for high-throughput particle imaging in microfluidic systems.

An Ultra-High-Throughput Flow-Focusing Microfluidic Device for Creation of Liquid Droplets in Air

2017 ◽  
Author(s):  
Pooyan Tirandazi ◽  
Gabriel Tomic ◽  
Carlos H. Hidrovo
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
High Speed ◽  
Large Scale ◽  
3D Structure ◽  
Scale Production ◽  
Liquid Droplets ◽  
Flow Focusing ◽  
Microfluidic Systems ◽  
Uniform Array

In this paper a new microfluidic technique is proposed for ultra-high-throughput generation of micron-sized water droplets using a high-speed air. We use a 3D flow-focusing microchannel fabricated in PDMS by multilayer lithography process. The interaction of liquid and gas created three main flow conditions which are: Flooded, Dripping, and Jetting. We characterize the Jetting regime where a capillary jet surrounded by the air breaks up into uniform array of droplets. Frequency of generation and droplet size are reported for the jetting regime under different liquid and gas flows. It was possible to obtain 25μm diameter droplets and much higher frequencies (f≈120 kHz) compared to the state-of-the-art microfluidic systems. We believe the advantages of this platform enables many novel applications such as high-throughput screening of airborne targets and large-scale production of oil-free particles. The 3D structure of this device also eliminates the limitation of the conventional droplet-based microfluidic systems, namely clogging issues due to particle aggregation.

Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Lab on a Chip ◽  
2017 ◽  
Vol 17 (22) ◽  
pp. 3736-3759 ◽  
Author(s):  
Matteo Cornaglia ◽  
Thomas Lehnert ◽  
Martin A. M. Gijs
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput ◽  
Microfluidic Devices ◽  
High Content Screening ◽  
Microfluidic Systems ◽  
Nematode Caenorhabditis Elegans ◽  
C Elegans

A review of the latest research on microfluidic devices forC. elegansanalysis reveals a clear potential for their fruitful application in high-throughput and high-content screening contexts.

A Deep Learning Model for Detection and Tracking in High-Throughput Images of Organoid

2021 ◽  
pp. 104490
Author(s):  
Xuesheng Bian ◽  
Gang Li ◽  
Cheng Wang ◽  
Weiquan Liu ◽  
Xiuhong Lin ◽  
...  
Keyword(s):  
Deep Learning ◽  
High Throughput ◽  
Learning Model ◽  
Detection And Tracking ◽  
Deep Learning Model

scholarly journals O23: CHARACTERISING PATIENT-DERIVED COLORECTAL CANCER TISSUE-ORIGINATED ORGANOIDAL SPHEROIDS FOR HIGH-THROUGHPUT MICROFLUIDIC APPLICATIONS

2021 ◽  
Vol 108 (Supplement_1) ◽  
Author(s):  
MI Khot ◽  
M Levenstein ◽  
R Coppo ◽  
J Kondo ◽  
M Inoue ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fluid Flow ◽  
High Throughput ◽  
Microfluidic Devices ◽  
In Vitro Models ◽  
Fluorescent Imaging ◽  
Cancer Tissue ◽  
Cell Models

Abstract Introduction Three-dimensional (3D) cell models have gained reputation as better representations of in vivo cancers as compared to monolayered cultures. Recently, patient tumour tissue-derived organoids have advanced the scope of complex in vitro models, by allowing patient-specific tumour cultures to be generated for developing new medicines and patient-tailored treatments. Integrating 3D cell and organoid culturing into microfluidics, can streamline traditional protocols and allow complex and precise high-throughput experiments to be performed with ease. Method Patient-derived colorectal cancer tissue-originated organoidal spheroids (CTOS) cultures were acquired from Kyoto University, Japan. CTOS were cultured in Matrigel and stem-cell media. CTOS were treated with 5-fluorouracil and cytotoxicity evaluated via fluorescent imaging and ATP assay. CTOS were embedded, sectioned and subjected to H&E staining and immunofluorescence for ABCG2 and Ki67 proteins. HT29 colorectal cancer spheroids were produced on microfluidic devices using cell suspensions and subjected to 5-fluorouracil treatment via fluid flow. Cytotoxicity was evaluated through fluorescent imaging and LDH assay. Result 5-fluorouracil dose-dependent reduction in cell viability was observed in CTOS cultures (p<0.01). Colorectal CTOS cultures retained the histology, tissue architecture and protein expression of the colonic epithelial structure. Uniform 3D HT29 spheroids were generated in the microfluidic devices. 5-fluorouracil treatment of spheroids and cytotoxic analysis was achieved conveniently through fluid flow. Conclusion Patient-derived CTOS are better complex models of in vivo cancers than 3D cell models and can improve the clinical translation of novel treatments. Microfluidics can streamline high-throughput screening and reduce the practical difficulties of conventional organoid and 3D cell culturing. Take-home message Organoids are the most advanced in vitro models of clinical cancers. Microfluidics can streamline and improve traditional laboratory experiments.

scholarly journals Fully Automated Cultivation of Adipose-Derived Stem Cells in the StemCellDiscovery—A Robotic Laboratory for Small-Scale, High-Throughput Cell Production Including Deep Learning-Based Confluence Estimation

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 575
Author(s):  
Jelena Ochs ◽  
Ferdinand Biermann ◽  
Tobias Piotrowski ◽  
Frederik Erkens ◽  
Bastian Nießing ◽  
...  
Keyword(s):  
Stem Cells ◽  
Deep Learning ◽  
High Throughput ◽  
High Speed ◽  
New Technologies ◽  
Human Mesenchymal Stem Cells ◽  
Simulation Software ◽  
System Capacity ◽  
Small Scale ◽  
Production Environment

Laboratory automation is a key driver in biotechnology and an enabler for powerful new technologies and applications. In particular, in the field of personalized therapies, automation in research and production is a prerequisite for achieving cost efficiency and broad availability of tailored treatments. For this reason, we present the StemCellDiscovery, a fully automated robotic laboratory for the cultivation of human mesenchymal stem cells (hMSCs) in small scale and in parallel. While the system can handle different kinds of adherent cells, here, we focus on the cultivation of adipose-derived hMSCs. The StemCellDiscovery provides an in-line visual quality control for automated confluence estimation, which is realized by combining high-speed microscopy with deep learning-based image processing. We demonstrate the feasibility of the algorithm to detect hMSCs in culture at different densities and calculate confluences based on the resulting image. Furthermore, we show that the StemCellDiscovery is capable of expanding adipose-derived hMSCs in a fully automated manner using the confluence estimation algorithm. In order to estimate the system capacity under high-throughput conditions, we modeled the production environment in a simulation software. The simulations of the production process indicate that the robotic laboratory is capable of handling more than 95 cell culture plates per day.

High-speed and high-precision fluorescence-based cell count and viability assays using the Cellaca™ MX high-throughput cell counter

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S97
Author(s):  
J. Bell ◽  
Y. Huang ◽  
S. Yung ◽  
H. Qazi ◽  
C. Hernandez ◽  
...  
Keyword(s):  
Cell Count ◽  
High Precision ◽  
High Throughput ◽  
High Speed ◽  
Cell Counter

scholarly journals Split and flow: reconfigurable capillary connection for digital microfluidic devices

Lab on a Chip ◽  
2014 ◽  
Vol 14 (18) ◽  
pp. 3589-3593 ◽  
Author(s):  
Florian Lapierre ◽  
Maxime Harnois ◽  
Yannick Coffinier ◽  
Rabah Boukherroub ◽  
Vincent Thomy
Keyword(s):  
Microfluidic Devices ◽  
Microfluidic Systems ◽  
Digital Microfluidic

How to take advantage of superhydrophobic microgrids to address the problem of coupling continuous to digital microfluidic systems? A reconfigurable capillary connection for digital microfluidic devices is presented.

High Speed FPGA Based 128-bit Advance Encryption Standard (AES)

2021 ◽  
Vol 11 ◽  
Author(s):  
Ibrahem M. T. Hamidi ◽  
Farah S. H. Al-aassi
Keyword(s):  
High Throughput ◽  
Field Programmable Gate Array ◽  
High Speed ◽  
Design Tool ◽  
Advanced Encryption Standard ◽  
Clock Frequency ◽  
Advance Encryption Standard ◽  
Field Programmable ◽  
Internal Component ◽  
Gate Array

Aim: Achieve high throughput 128 bits FPGA based Advanced Encryption Standard. Background: Field Programmable Gate Array (FPGA) provides an efficient platform for design AES cryptography system. It provides the capability to control over each bit using HDL programming language such as VHDL and Verilog which results an output speed in Gbps rang. Objective: Use Field Programmable Gate Array (FPGA) to design high throughput 128 bits FPGA based Advanced Encryption Standard. Method: Pipelining technique has used to achieve maximum possible speed. The level of pipelining includes round pipelining and internal component pipelining where number of registers inserted in particular places to increase the output speed. The proposed design uses combinatorial logic to implement the byte substitution. The s-box implemented using composed field arithmetic with 7 stages of pipelining to reduce the combinatorial logic level. The presented model has implemented using VHDL in Xilinix ISETM 14.4 design tool. Result: The achieved results were 18.55 Gbps at a clock frequency of 144.96 MHz and area of 1568 Slices in Spartan3 xc3s1000 hardware. Conclusion: The results show that the proposed design reaches a high throughput with acceptable area usage compare with other designs in the literature.

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