In vivo three dimensional cuticle intact Drosophila brain imaging using laser scanning optical resolution photoacoustic microscopy

2019 ◽  
Author(s):  
Kai-Yao Chang ◽  
Hsuan Ou-Yang ◽  
Pei-Shan Ho ◽  
Shun-Chi Wu ◽  
Yen-Yin Lin ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Optical Resolution ◽  
Photoacoustic Microscopy ◽  
Drosophila Brain

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

Laser-scanning optical-resolution photoacoustic microscopy

2009 ◽  
Vol 34 (12) ◽  
pp. 1771 ◽  
Author(s):  
Zhixing Xie ◽  
Shuliang Jiao ◽  
Hao F. Zhang ◽  
Carmen A. Puliafito
Keyword(s):  
Laser Scanning ◽  
Optical Resolution ◽  
Photoacoustic Microscopy
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