Qualitative image comparison between in vivo endoscopic optical coherence tomography and conventional histology of the healthy human oral mucosa

2019 ◽  
Author(s):  
Julia Walther ◽  
Christian Schnabel ◽  
Marius Albrecht ◽  
Edmund Koch
Keyword(s):  
Optical Coherence Tomography ◽  
Oral Mucosa ◽  
Optical Coherence ◽  
Image Comparison ◽  
Healthy Human ◽  
Endoscopic Optical Coherence Tomography ◽  
Conventional Histology

scholarly journals In Vivo Endoscopic Optical Coherence Tomography of the Healthy Human Oral Mucosa: Qualitative and Quantitative Image Analysis

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 827
Author(s):  
Marius Albrecht ◽  
Christian Schnabel ◽  
Juliane Mueller ◽  
Jonas Golde ◽  
Edmund Koch ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Image Analysis ◽  
Soft Tissue ◽  
Oral Mucosa ◽  
Cross Sections ◽  
Optical Coherence ◽  
Healthy Human ◽  
Quantitative Image ◽  
Endoscopic Optical Coherence Tomography

To date, there is still a lack of reliable imaging modalities to improve the quality of consultation, diagnostic and medical examinations of the oral mucosa in dentistry. Even though, optical technologies have become an important element for the detection and treatment of different diseases of soft tissue, for the case of oral screenings the evidence of the benefit in comparison to conventional histopathology is mostly still pending. One promising optical technology for oral diagnostics is optical coherence tomography (OCT). To prove the potential of OCT, even the amount of freely accessible OCT data is not sufficient to describe the variance of healthy human oral soft tissue in vivo. In order to remedy this deficiency, the present study provides in vivo OCT cross sections of the human oral mucosa of the anterior and posterior oral cavity as well as the oropharynx of 47 adult volunteers. A collection of representative OCT cross sections forms the basis for a randomized blinded image analysis by means of seven criteria to assess the main features of the superficial layers of the human oral mucosa and to determine its correlation to regional features known from hematoxylin and eosin (HE) stained histology.

scholarly journals Polarization sensitive optical coherence tomography with single input for imaging depth-resolved collagen organizations

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Peijun Tang ◽  
Mitchell A. Kirby ◽  
Nhan Le ◽  
Yuandong Li ◽  
Nicole Zeinstra ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Polarization State ◽  
Transmission Model ◽  
Optical Coherence ◽  
Healthy Human ◽  
Collagen Organization ◽  
Single Input

AbstractCollagen organization plays an important role in maintaining structural integrity and determining tissue function. Polarization-sensitive optical coherence tomography (PSOCT) is a promising noninvasive three-dimensional imaging tool for mapping collagen organization in vivo. While PSOCT systems with multiple polarization inputs have demonstrated the ability to visualize depth-resolved collagen organization, systems, which use a single input polarization state have not yet demonstrated sufficient reconstruction quality. Herein we describe a PSOCT based polarization state transmission model that reveals the depth-dependent polarization state evolution of light backscattered within a birefringent sample. Based on this model, we propose a polarization state tracing method that relies on a discrete differential geometric analysis of the evolution of the polarization state in depth along the Poincare sphere for depth-resolved birefringent imaging using only one single input polarization state. We demonstrate the ability of this method to visualize depth-resolved myocardial architecture in both healthy and infarcted rodent hearts (ex vivo) and collagen structures responsible for skin tension lines at various anatomical locations on the face of a healthy human volunteer (in vivo).

scholarly journals Optical coherence tomography imaging of oral mucosa bullous diseases: a preliminary study

2020 ◽  
Vol 49 (2) ◽  
pp. 20190071
Author(s):  
Dario Di Stasio ◽  
Dorina Lauritano ◽  
Francesca Loffredo ◽  
Enrica Gentile ◽  
Fedora Della Vella ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Oral Mucosa ◽  
Statistical Power ◽  
Pemphigus Vulgaris ◽  
Invasive Technique ◽  
Optical Coherence ◽  
Non Invasive ◽  
Bullous Diseases ◽  
Subepithelial Layer

Objectives: Optical coherence tomography (OCT) is a non-invasive technique based on optical imaging with a micrometre resolution. The purpose of this study is to investigate the potential role of OCT in evaluating oral mucosa bullous diseases. Methods: two patients with bullous pemphigoid (BP) and one patient with pemphigus vulgaris (PV) were examined and images of their oral lesions were performed using OCT. Results: In OCT images, the BP blister has a clearly different morphology from the PV one compared to the blistering level. Conclusion: This exploratory study suggests that the OCT is able to distinguish epithelial and subepithelial layer in vivo images of healthy oral mucosa from those with bullous diseases, assisting the clinicians in differential diagnosis.The presented data are in accordance with the scientific literature, although a wider pool of cases is needed to increase statistical power. Histological examination and immunofluorescence methods remain the gold standard for the diagnosis of oral bullous diseases. In this context, the OCT can provide the clinician with a valuable aid both as an additional diagnostic tool and in the follow up of the disease.

High-Speed In Vivo Endoscopic Optical Coherence Tomography

1999 ◽  
Author(s):  
Joseph A. Izatt ◽  
Andrew M. Rollins ◽  
Rujchai Ung-arunyawee ◽  
Amitabh Chak ◽  
Richard C. Wong ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Speed ◽  
Optical Coherence ◽  
Endoscopic Optical Coherence Tomography
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