Cell imaging with squaraine dye based on two-photon excitation fluorescence imaging

2018 ◽  
Author(s):  
Liwei Liu ◽  
Junxian Geng ◽  
Rongxing Yi ◽  
Junle Qu
Keyword(s):  
Fluorescence Imaging ◽  
Cell Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Squaraine Dye ◽  
Two Photon Excitation

Lysosome-targetable polythiophene nanoparticles for two-photon excitation photodynamic therapy and deep tissue imaging

2017 ◽  
Vol 5 (20) ◽  
pp. 3651-3657 ◽  
Author(s):  
Shaojing Zhao ◽  
Guangle Niu ◽  
Feng Wu ◽  
Li Yan ◽  
Hongyan Zhang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Quantum Yield ◽  
Fluorescence Imaging ◽  
Cross Section ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon ◽  
Photon Excitation ◽  
Deep Tissue Imaging ◽  
Two Photon Excitation

Polythiophene nanoparticles with large TPA cross section and high1O2generation quantum yield have been developed for simultaneous lysosome-targetable fluorescence imaging and photodynamic therapy.

scholarly journals In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

2014 ◽  
Vol 26 (1) ◽  
pp. 25-30
Author(s):  
Yoko Mizuta ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Plant Tissues ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Fluorescence and fluorescence imaging of two Schiff derivatives sensitive to Fe3+ induced by single- and two-photon excitation

2013 ◽  
Vol 176 ◽  
pp. 488-496 ◽  
Author(s):  
Xiang-long Meng ◽  
Xian Zhang ◽  
Jin-shui Yao ◽  
Jing-jing Zhang ◽  
Bi-yan Ding
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

High Speed Two Photon Excitation Microscopy in Live Cell Imaging using Image Correlation Spectroscopy (ICS)

2001 ◽  
Vol 7 (S2) ◽  
pp. 22-23
Author(s):  
P. W. Wiseman ◽  
J. C. Bouwer ◽  
S. Peltier ◽  
M. H. Ellisman
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Live Cell ◽  
Image Correlation ◽  
Scanning System ◽  
Optical Sectioning ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

For live-cell imaging, two-photon excitation microscopy (TPEM) is proving to be a significant technological advancement. The unique features offered by TPEM are the ability to image thick sections, excellent optical sectioning capabilities, low damage to living cells, and less out of focus fluorescence and out of focus photobleaching. of these features, the most useful for the biological microscopist, is optical sectioning. Optical sectioning is an intrinsic property of the two-photon process, whereby, two infrared (IR) photons are absorbed quickly to excite a single UV/blue transition. The probability for exciting a two photon transition is proportional to the instantaneous excitation intensity squared. Therefore, for a focused laser beam, only light at the focal point of the excitation beam excites a fluorescent transition. Thus, the need for confocal apertures and time consuming deconvolution algorithms are, for the most part, eliminated.We have continued to develop and enhance our ability to perform high-speed, two-photon excitation fluorescence microscopy. in 1998, we successfully deployed a prototype, video-rate twophoton laser scanning system (30 frames/sec or faster at reduced scan width) developed with support from Nikon Corporation. That system was built upon a Nikon RCM 8000 confocal microscope.

Conjugated-Polymer-Based Red-Emitting Nanoparticles for Two-Photon Excitation Cell Imaging with High Contrast

Langmuir ◽  
2014 ◽  
Vol 30 (26) ◽  
pp. 7623-7627 ◽  
Author(s):  
Shuang Li ◽  
Xiaoqin Shen ◽  
Lin Li ◽  
Peiyan Yuan ◽  
Zhenping Guan ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Cell Imaging ◽  
High Contrast ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
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