Conjugated-Polymer-Based Red-Emitting Nanoparticles for Two-Photon Excitation Cell Imaging with High Contrast

Langmuir ◽  
2014 ◽  
Vol 30 (26) ◽  
pp. 7623-7627 ◽  
Author(s):  
Shuang Li ◽  
Xiaoqin Shen ◽  
Lin Li ◽  
Peiyan Yuan ◽  
Zhenping Guan ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Cell Imaging ◽  
High Contrast ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

2014 ◽  
Vol 26 (1) ◽  
pp. 25-30
Author(s):  
Yoko Mizuta ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Plant Tissues ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

High Speed Two Photon Excitation Microscopy in Live Cell Imaging using Image Correlation Spectroscopy (ICS)

2001 ◽  
Vol 7 (S2) ◽  
pp. 22-23
Author(s):  
P. W. Wiseman ◽  
J. C. Bouwer ◽  
S. Peltier ◽  
M. H. Ellisman
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Live Cell ◽  
Image Correlation ◽  
Scanning System ◽  
Optical Sectioning ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

For live-cell imaging, two-photon excitation microscopy (TPEM) is proving to be a significant technological advancement. The unique features offered by TPEM are the ability to image thick sections, excellent optical sectioning capabilities, low damage to living cells, and less out of focus fluorescence and out of focus photobleaching. of these features, the most useful for the biological microscopist, is optical sectioning. Optical sectioning is an intrinsic property of the two-photon process, whereby, two infrared (IR) photons are absorbed quickly to excite a single UV/blue transition. The probability for exciting a two photon transition is proportional to the instantaneous excitation intensity squared. Therefore, for a focused laser beam, only light at the focal point of the excitation beam excites a fluorescent transition. Thus, the need for confocal apertures and time consuming deconvolution algorithms are, for the most part, eliminated.We have continued to develop and enhance our ability to perform high-speed, two-photon excitation fluorescence microscopy. in 1998, we successfully deployed a prototype, video-rate twophoton laser scanning system (30 frames/sec or faster at reduced scan width) developed with support from Nikon Corporation. That system was built upon a Nikon RCM 8000 confocal microscope.

Gold nanorod enhanced conjugated polymer/photosensitizer composite nanoparticles for simultaneous two-photon excitation fluorescence imaging and photodynamic therapy

Nanoscale ◽  
2019 ◽  
Vol 11 (41) ◽  
pp. 19551-19560 ◽  
Author(s):  
Shuang Li ◽  
Xiaoqin Shen ◽  
Qing-Hua Xu ◽  
Yong Cao
Keyword(s):  
Photodynamic Therapy ◽  
Energy Transfer ◽  
Singlet Oxygen ◽  
Plasmon Resonance ◽  
Conjugated Polymer ◽  
Oxygen Generation ◽  
Two Photon ◽  
Singlet Oxygen Generation ◽  
Photon Excitation ◽  
Two Photon Excitation

Plasmon resonance and energy transfer are integrated to prepare nano-photosensitizers with enhanced two-photon excitation fluorescence and singlet oxygen generation ability.

scholarly journals Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging

2019 ◽  
Vol 25 (01) ◽  
pp. 1 ◽  
Author(s):  
Ryosuke Oketani ◽  
Haruka Suda ◽  
Kumiko Uegaki ◽  
Toshiki Kubo ◽  
Tomoki Matsuda ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Two Photon ◽  
Visible Wavelength ◽  
Photon Excitation ◽  
Two Photon Excitation

Photosensitizer-doped conjugated polymer nanoparticles with high cross-sections for one- and two-photon excitation

Nanoscale ◽  
2011 ◽  
Vol 3 (4) ◽  
pp. 1451 ◽  
Author(s):  
Jennifer L. Grimland ◽  
Changfeng Wu ◽  
Ria R. Ramoutar ◽  
Julia L. Brumaghim ◽  
Jason McNeill
Keyword(s):  
Cross Sections ◽  
Conjugated Polymer ◽  
Polymer Nanoparticles ◽  
Two Photon ◽  
Photon Excitation ◽  
Conjugated Polymer Nanoparticles ◽  
Two Photon Excitation
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