In vivo monitoring optical clearing process of skin using two-photon microscopy

2018 ◽  
Author(s):  
Wei Feng ◽  
Tingting Yu ◽  
Dan Zhu ◽  
Lu Deng
Keyword(s):  
Optical Clearing ◽  
In Vivo Monitoring ◽  
Two Photon Microscopy ◽  
Two Photon

In vivo monitoring of intracellular chloroplast movements in intact leaves of c4plants using two-photon microscopy

2014 ◽  
Vol 77 (10) ◽  
pp. 806-813
Author(s):  
Jeongeun Ryu ◽  
Hyoseok Nam ◽  
Hae Koo Kim ◽  
Yongjoon Joo ◽  
Sang Joon Lee ◽  
...  
Keyword(s):  
In Vivo Monitoring ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Chloroplast Movements ◽  
Intact Leaves

scholarly journals Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution

2017 ◽  
Vol 7 (2) ◽  
pp. 17153-17153 ◽  
Author(s):  
Yan-Jie Zhao ◽  
Ting-Ting Yu ◽  
Chao Zhang ◽  
Zhao Li ◽  
Qing-Ming Luo ◽  
...  
Keyword(s):  
Dendritic Spines ◽  
In Vivo Imaging ◽  
Great Promise ◽  
Critical Periods ◽  
Optical Clearing ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Neuroscience Research ◽  
Mouse Cortex

Abstract Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here, we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy, this technique allowed us to repeatedly image neurons, microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation. Given its easy handling and safety, this method holds great promise for application in neuroscience research.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

Sensory Transmission is Bi-directionally Modulated by Astrocytic Ca2+ in Barrel Cortex of Behaving Mice

2021 ◽  
Author(s):  
Simeng Gu ◽  
Wei Wang ◽  
Kuan Zhang ◽  
Rou Feng ◽  
Naling Li ◽  
...  
Keyword(s):  
Sensory Input ◽  
Barrel Cortex ◽  
Synaptic Function ◽  
Metabolic Clearance ◽  
Sleep State ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Sensory Transmission

Abstract Different effects of astrocyte during sleep and awake have been extensively studied, especially for metabolic clearance by the glymphatic system, which works during sleep and stops working during waking states. However, how astrocytes contribute to modulation of sensory transmission during sleep and awake animals remain largely unknown. Recent advances in genetically encoded Ca2+ indicators have provided a wealth of information on astrocytic Ca2+, especially in their fine perisynaptic processes, where astrocytic Ca2+ most likely affects the synaptic function. Here we use two-photon microscopy to image astrocytic Ca2+ signaling in freely moving mice trained to run on a wheel in combination with in vivo whole-cell recordings to evaluate the role of astrocytic Ca2+ signaling in different behavior states. We found that there are two kinds of astrocytic Ca2+ signaling: a small long-lasting Ca2+ increase during sleep state and a sharp widespread but short-long-lasting Ca2+ spike when the animal was awake (fluorescence increases were 23.2 ± 14.4% for whisker stimulation at sleep state, compared with 73.3 ± 11.7% for at awake state, paired t-test, p < 0.01). The small Ca2+ transients decreased extracellular K+, hyperpolarized the neurons, and suppressed sensory transmission; while the large Ca2+ wave enhanced sensory input, contributing to reliable sensory transmission in aroused states. Locus coeruleus activation works as a switch between these two kinds of astrocytic Ca2+ elevation. Thus, we show that cortical astrocytes play an important role in processing of sensory input. These two types of events appear to have different pharmacological sources and may play a different role in facilitating the efficacy of sensory transmission.

Quantum Dots assisted and NIR-II Emissive In Vivo Two-photon microscopy

2021 ◽  
Author(s):  
Huwei Ni ◽  
Yalun Wang ◽  
Tao Tang ◽  
Wenbin Yu ◽  
Dongyu Li ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Two Photon Microscopy ◽  
Two Photon

Video-rate two-photon microscopy of cortical hemodynamics in-vivo

2006 ◽  
Author(s):  
Matthew Bouchard ◽  
Svetlana Ruvinskya ◽  
David A. Boas ◽  
Elizabeth M. C. Hillman
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Impact of Body Site, Age, and Gender on the Collagen/Elastin Index by Noninvasive in vivo Vertical Two-Photon Microscopy

2017 ◽  
Vol 30 (5) ◽  
pp. 260-267 ◽  
Author(s):  
Carolin Czekalla ◽  
Karl-Heinz Schönborn ◽  
Nadine Döge ◽  
Sora Jung ◽  
Maxim E. Darvin ◽  
...  
Keyword(s):  
Body Site ◽  
Age And Gender ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
And Gender

scholarly journals P02.08 Visualizing tumor cell - lymphocyte interactions in the brain metastatic cascade using in vivo two photon microscopy

2018 ◽  
Vol 20 (suppl_3) ◽  
pp. iii273-iii273
Author(s):  
M Piechutta ◽  
A S Berghoff ◽  
M A Karreman ◽  
K Gunkel ◽  
W Wick ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Metastatic Cascade ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
The Brain
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