scholarly journals Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution

2017 ◽  
Vol 7 (2) ◽  
pp. 17153-17153 ◽  
Author(s):  
Yan-Jie Zhao ◽  
Ting-Ting Yu ◽  
Chao Zhang ◽  
Zhao Li ◽  
Qing-Ming Luo ◽  
...  
Keyword(s):  
Dendritic Spines ◽  
In Vivo Imaging ◽  
Great Promise ◽  
Critical Periods ◽  
Optical Clearing ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Neuroscience Research ◽  
Mouse Cortex

Abstract Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here, we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy, this technique allowed us to repeatedly image neurons, microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation. Given its easy handling and safety, this method holds great promise for application in neuroscience research.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 3303 ◽  
Author(s):  
Seoyeon Bok ◽  
Taejun Wang ◽  
Chan-Ju Lee ◽  
Seong-Uk Jeon ◽  
Young-Eun Kim ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Mouse Model ◽  
In Vivo Imaging ◽  
Focal Cerebral Ischemia ◽  
Activated Microglia ◽  
Two Photon Microscopy ◽  
Two Photon

Photostable far-red emitting pluronic silicate nanoparticles: perfect blood pool fluorophores for biphotonic in vivo imaging of the leaky tumour vasculature

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 94200-94205 ◽  
Author(s):  
Zheng Zheng ◽  
Flavien Caraguel ◽  
Yuan-Yuan Liao ◽  
Chantal Andraud ◽  
Boudewijn van der Sanden ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Blood Pool ◽  
Pluronic F127 ◽  
Photon Absorption ◽  
Absorption Properties ◽  
Two Photon Absorption ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Tumour Vasculature

A new non-diffusible fluorescent probe for two photon microscopy, comprising a hydrophobic push-pull dye in the apolar core of Pluronic F127–silica nanoparticles, shows intense red emission (Φf 39% at 650 nm) and two-photon absorption properties in the NIR.

Upconverting nanoparticles: a versatile platform for wide-field two-photon microscopy and multi-modal in vivo imaging

2015 ◽  
Vol 44 (6) ◽  
pp. 1302-1317 ◽  
Author(s):  
Yong Il Park ◽  
Kang Taek Lee ◽  
Yung Doug Suh ◽  
Taeghwan Hyeon
Keyword(s):  
In Vivo Imaging ◽  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon

Upconverting nanoparticles (UCNPs) enable the establishment of a novel UCNP-based platform for wide-field two-photon microscopy and multimodal in vivo imaging.

scholarly journals In Vivo Imaging of T-Cell Motility in the Elicitation Phase of Contact Hypersensitivity Using Two-Photon Microscopy

2011 ◽  
Vol 131 (4) ◽  
pp. 977-979 ◽  
Author(s):  
Gyohei Egawa ◽  
Tetsuya Honda ◽  
Hideaki Tanizaki ◽  
Hiromi Doi ◽  
Yoshiki Miyachi ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Motility ◽  
In Vivo Imaging ◽  
Contact Hypersensitivity ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals In Vivo Imaging of Neocortical and Hippocampal CA1 Neurons by Two-photon Microscopy

2014 ◽  
Vol 54 (1) ◽  
pp. 035-038
Author(s):  
Ryosuke KAWAKAMI ◽  
Terumasa HIBI ◽  
Tomomi NEMOTO
Keyword(s):  
In Vivo Imaging ◽  
Ca1 Neurons ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Hippocampal Ca1 Neurons ◽  
Hippocampal Ca1

scholarly journals Transient Effects of Anesthetics on Dendritic Spines and Filopodia in the Living Mouse Cortex

2011 ◽  
Vol 115 (4) ◽  
pp. 718-726 ◽  
Author(s):  
Guang Yang ◽  
Paul C. Chang ◽  
Alex Bekker ◽  
Thomas J.J. Blanck ◽  
Wen-Biao Gan
Keyword(s):  
Dendritic Spines ◽  
Pyramidal Neurons ◽  
Excitatory Synapses ◽  
Transient Effects ◽  
Yellow Fluorescence Protein ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Mouse Cortex ◽  
Living Mouse ◽  
Fluorescence Protein

Background Anesthetics are widely used to induce unconsciousness, pain relief, and immobility during surgery. It remains unclear whether the use of anesthetics has significant and long-lasting effects on synapse development and plasticity in the brain. To address this question, the authors examined the formation and elimination of dendritic spines, postsynaptic sites of excitatory synapses, in the developing mouse cortex during and after anesthetics exposure. Methods Transgenic mice expressing yellow fluorescence protein in layer 5 pyramidal neurons were used in this study. Mice at 1 month of age underwent ketamine-xylazine and isoflurane anesthesia over a period of hours. The elimination and formation rates of dendritic spines and filopodia, the precursors of spines, were followed over hours to days in the primary somatosensory cortex using transcranial two-photon microscopy. Four to five animals were examined under each experimental condition. Student t test and Mann-Whitney U test were used to analyze the data. Results Administration of either ketamine-xylazine or isoflurane rapidly altered dendritic filopodial dynamics but had no significant effects on spine dynamics. Ketamine-xylazine increased filopodial formation whereas isoflurane decreased filopodial elimination during 4 h of anesthesia. Both effects were transient and disappeared within a day after the animals woke up. Conclusion Studies suggest that exposure to anesthetics transiently affects the dynamics of dendritic filopodia but has no significant effect on dendritic spine development and plasticity in the cortex of 1-month-old mice.

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