scholarly journals Impact of Body Site, Age, and Gender on the Collagen/Elastin Index by Noninvasive in vivo Vertical Two-Photon Microscopy

2017 ◽  
Vol 30 (5) ◽  
pp. 260-267 ◽  
Author(s):  
Carolin Czekalla ◽  
Karl-Heinz Schönborn ◽  
Nadine Döge ◽  
Sora Jung ◽  
Maxim E. Darvin ◽  
...  
Keyword(s):  
Body Site ◽  
Age And Gender ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
And Gender

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

Sensory Transmission is Bi-directionally Modulated by Astrocytic Ca2+ in Barrel Cortex of Behaving Mice

2021 ◽  
Author(s):  
Simeng Gu ◽  
Wei Wang ◽  
Kuan Zhang ◽  
Rou Feng ◽  
Naling Li ◽  
...  
Keyword(s):  
Sensory Input ◽  
Barrel Cortex ◽  
Synaptic Function ◽  
Metabolic Clearance ◽  
Sleep State ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Sensory Transmission

Abstract Different effects of astrocyte during sleep and awake have been extensively studied, especially for metabolic clearance by the glymphatic system, which works during sleep and stops working during waking states. However, how astrocytes contribute to modulation of sensory transmission during sleep and awake animals remain largely unknown. Recent advances in genetically encoded Ca2+ indicators have provided a wealth of information on astrocytic Ca2+, especially in their fine perisynaptic processes, where astrocytic Ca2+ most likely affects the synaptic function. Here we use two-photon microscopy to image astrocytic Ca2+ signaling in freely moving mice trained to run on a wheel in combination with in vivo whole-cell recordings to evaluate the role of astrocytic Ca2+ signaling in different behavior states. We found that there are two kinds of astrocytic Ca2+ signaling: a small long-lasting Ca2+ increase during sleep state and a sharp widespread but short-long-lasting Ca2+ spike when the animal was awake (fluorescence increases were 23.2 ± 14.4% for whisker stimulation at sleep state, compared with 73.3 ± 11.7% for at awake state, paired t-test, p < 0.01). The small Ca2+ transients decreased extracellular K+, hyperpolarized the neurons, and suppressed sensory transmission; while the large Ca2+ wave enhanced sensory input, contributing to reliable sensory transmission in aroused states. Locus coeruleus activation works as a switch between these two kinds of astrocytic Ca2+ elevation. Thus, we show that cortical astrocytes play an important role in processing of sensory input. These two types of events appear to have different pharmacological sources and may play a different role in facilitating the efficacy of sensory transmission.

Quantum Dots assisted and NIR-II Emissive In Vivo Two-photon microscopy

2021 ◽  
Author(s):  
Huwei Ni ◽  
Yalun Wang ◽  
Tao Tang ◽  
Wenbin Yu ◽  
Dongyu Li ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Two Photon Microscopy ◽  
Two Photon

Video-rate two-photon microscopy of cortical hemodynamics in-vivo

2006 ◽  
Author(s):  
Matthew Bouchard ◽  
Svetlana Ruvinskya ◽  
David A. Boas ◽  
Elizabeth M. C. Hillman
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

scholarly journals P02.08 Visualizing tumor cell - lymphocyte interactions in the brain metastatic cascade using in vivo two photon microscopy

2018 ◽  
Vol 20 (suppl_3) ◽  
pp. iii273-iii273
Author(s):  
M Piechutta ◽  
A S Berghoff ◽  
M A Karreman ◽  
K Gunkel ◽  
W Wick ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Metastatic Cascade ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
The Brain

scholarly journals Hyperspectral in vivo two-photon microscopy of intrinsic contrast

2008 ◽  
Vol 33 (18) ◽  
pp. 2164 ◽  
Author(s):  
Andrew J. Radosevich ◽  
Matthew B. Bouchard ◽  
Sean A. Burgess ◽  
Brenda R. Chen ◽  
Elizabeth M. C. Hillman
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

Failure of Demonstrated Clinical Efficacy of Antibiotic-Bonded Continuous Ambulatory Peritoneal Dialysis (CAPD) Catheters

1990 ◽  
Vol 10 (1) ◽  
pp. 57-59 ◽  
Author(s):  
Stanley Z. Trooskin ◽  
Richard A. Harvey ◽  
T. w. J. Lennard ◽  
Ralph S. Greco
Keyword(s):  
Clinical Trial ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Infectious Complications ◽  
Clinical Report ◽  
Age And Gender ◽  
And Gender ◽  
To Receive

Previous in vitro, in vivo, and a preliminary clinical report have demonstrated efficacy of noncovalently bonding antibiotics to the surface of continuous ambulatory peritoneal dialysis (CAPO) catheters in decreasing infectious complications. A larger prospective randomized clinical trial was completed. Eighty-six patients with chronic renal failure were enrolled in the study and randomized to receive either a surfactant treated or untreated control catheter. All catheters were soaked in cefoxitin at the time of insertion. Groups were comparable in terms of pre-existing illnesses, age, and gender. No differences were shown in the incidence of cathetertract infections, peritonitis or mechanical complications. There was also no differences in microbiologic culture results. Therefore, it is concluded that this clinical trial did not demonstrate a reduction in catheter-related infectious complications by antibiotic bonding.

Sign in / Sign up

Export Citation Format

Share Document