Nanoparticles manipulation on plasmonic metasurface for determination of antibody in lab-on-a-chip devices

2018 ◽  
Author(s):  
Min Jiang ◽  
Wenhao Xu ◽  
Guanghui Wang ◽  
Xuping Zhang ◽  
Feng Wang
Keyword(s):  
Lab On A Chip

Lab-on-a-Chip Device for Hydrogen Sulfide Sensing in Biomedical and Environmental Applications Using Electrochemical Approach

2015 ◽  
Author(s):  
A. Baniya ◽  
S. Thapa ◽  
E. Borquist ◽  
D. Bailey ◽  
D. Wood ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Direct Detection ◽  
Sulfide Oxidation ◽  
Lab On A Chip ◽  
Electrode System ◽  
Sulfide Concentration ◽  
Pt Electrodes ◽  
Electrochemical Approach ◽  
And Performance

Hydrogen sulfide (H2S) is rapidly emerging as a biologically significant signaling molecule. In recent studies, sulfide level in blood or plasma has been reported to be in the concentration between 10 and 300 μM suggesting it acts in various diseases. This work reports progress on a new Lab-on-a-Chip (LOC) device for these applications. The uniquely designed, hand-held device uses advanced liberation chemistry that releases H2S from liquid sample and an electrochemical approach to detect sulfide concentration from the aqueous solution. The device itself consists of three distinct layers of Polydimethylsiloxane (PDMS) structures and a three electrode system for direct and rapid H2S concentration measurement. In this work specifically, the oxidation of sulfide at the gold (Au) and platinum (Pt.) electrodes has been examined. This is the first known application of electrochemical H2S sensing in an LOC application. The analytical utility and performance of the device has been assessed through direct detection using chronoamperometry (CA) scan and cyclic voltammetry (CV). An electrocatalytic sulfide oxidation signal has been recorded for sulfide concentration range vs, Ag/AgCl at different pH buffers at the trapping chamber. The calibration curve in the range 1 μM to 1 M was obtained using this electrode setup. The detection limit was found to be 0.1 μM. This device shows promise for providing fast and inexpensive determination of H2S concentration in aqueous samples.

Determination of pore-scale hydrate phase equilibria in sediments using lab-on-a-chip technology

Lab on a Chip ◽  
2017 ◽  
Vol 17 (23) ◽  
pp. 4070-4076 ◽  
Author(s):  
Stian Almenningen ◽  
Josef Flatlandsmo ◽  
Anthony R. Kovscek ◽  
Geir Ersland ◽  
Martin A. Fernø
Keyword(s):  
Porous Media ◽  
Phase Equilibria ◽  
Lab On A Chip ◽  
Pore Scale ◽  
Experimental Protocol ◽  
Fast Determination ◽  
Chip Technology ◽  
Hydrate Phase ◽  
Hydrate Stability

We present an experimental protocol for fast determination of hydrate stability in porous media for a range of pressure and temperature conditions.

scholarly journals Temperature and Denaturing Substances Influence on Lab-on-a-Chip Electrophoresis

2009 ◽  
Vol 28 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Olgica Trenčevska ◽  
Vasko Aleksovski ◽  
Kiro Stojanoski
Keyword(s):  
Neurological Disorders ◽  
Incubation Temperature ◽  
Serum Proteins ◽  
Biological Fluids ◽  
Lab On A Chip ◽  
Serum Samples ◽  
Qualitative And Quantitative ◽  
Incubation Temperatures ◽  
Short Time

Temperature and Denaturing Substances Influence on Lab-on-a-Chip Electrophoresis Qualitative and quantitative determination of proteins in different biological fluids is of great significance in medicine, due to their importance in diagnosis and treatment of some diseases. Nowadays, different methods for protein analysis are available. Lab-on-a-chip electrophoresis is a relatively new technique, based on microfluidics, which allows samples of biological fluids to be analyzed within a microchip. This paper describes the optimization of performance of the chip-based protein analyses in serum samples from patients with different neurological disorders. Using microchip technology, serum proteins with the molecular mass from 4.5 to 240 Kb were separated and sized. The fluorescence detection method in the analysis was used to follow the influence of the temperature and the type and concentration of denaturing substances on the electrophoresis protein profiles. It was noted that, depending on incubation temperature and denaturing substances, different electrophoresis patterns can be obtained from the proteins of one specimen. Significant change of the fluorescence intensity was observed when different incubation temperatures were used, probably due to fluorescence quenching. In some cases, the band intensity was changed several times. Lab-on-a-chip electrophoresis is a very efficient method for the separation and determination of different serum proteins in a very short time. However, to obtain comparable results for the analysis, the denaturing agent concentration and temperature must be observed and maintained carefully.

Spectrometric Determination of the Refractive Index of Optical Wave Guiding Materials Used in Lab-on-a-Chip Applications

2006 ◽  
Vol 60 (3) ◽  
pp. 322-329 ◽  
Author(s):  
Andres M. Cardenas-Valencia ◽  
Jay Dlutowski ◽  
David Fries ◽  
Larry Langebrake
Keyword(s):  
Refractive Index ◽  
Lab On A Chip ◽  
Optical Wave ◽  
Materials Used

scholarly journals Rapid determination of vitamin B12 concentration with a chemiluminescence lab on a chip

Lab on a Chip ◽  
2012 ◽  
Vol 12 (13) ◽  
pp. 2353 ◽  
Author(s):  
Khoi Seng Lok ◽  
Siti Zubaidah binte Abdul Muttalib ◽  
Peter Peng Foo Lee ◽  
Yien Chian Kwok ◽  
Nam-Trung Nguyen
Keyword(s):  
Vitamin B12 ◽  
Rapid Determination ◽  
Lab On A Chip

Lab-on-a-chip technology for determination of protein isoform profiles

2006 ◽  
Vol 1127 (1-2) ◽  
pp. 175-182 ◽  
Author(s):  
Maria Lönnberg ◽  
Jan Carlsson
Keyword(s):  
Lab On A Chip ◽  
Chip Technology ◽  
Protein Isoform

scholarly journals Electrophoresis as a method for characterization of protein changes in maize after pelleting process

2013 ◽  
Vol 19 (2) ◽  
pp. 221-229
Author(s):  
Radmilo Colovic ◽  
Aleksandra Torbica ◽  
Dusica Ivanov ◽  
Jelena Tomic ◽  
Djuro Vukmirovic ◽  
...  
Keyword(s):  
Retention Time ◽  
Protein Solubility ◽  
Lab On A Chip ◽  
Raw Material ◽  
Hammer Mill ◽  
Protein Modifications ◽  
Maize Protein ◽  
High Level ◽  
Physical Changes

One of the most important crops used for feeding of animals is maize (Zea mays L). Kernels of maize contain 7-13 g of protein/100 g of dry matter, and they are protagonist of chemical and physical changes during the processing of raw material. There are several methods for determination of protein changes during thermal processing. The objective of this study was to investigate application of Lab-on-a-Chip electrophoresis as a method for qualitative and quantitative determination of maize protein modifications during pelleting. Parameters which were varied during pelleting process were diameter of sieve openings of the hammer mill and duration of steam conditioning process. According to the results, conditioning retention time of 5 min could be considered as mild in terms of protein changes for samples ground to pass 3 and 4 mm sieve. Conditioning retention time of 10 min was long enough for achieving of high level of protein modifications for all samples. Regression analysis of obtained data showed that both independent parameters had significant (p < 0.05) influence on response variable (protein solubility). Protein electrophoregrams of investigated samples were qualitatively nearly identical, but quantitative differences occurred, especially in the range 18-85 kDa of protein molecular weight (MW). Results in this paper have proven that Lab-on-a-Chip electrophoresis registered changes in protein structure even when protein solubility was not changed, unlike other conventional methods. This method can be used for determination of degree of protein changes during pelleting process with remarkable certainty.

Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties

2010 ◽  
Vol 58 (13) ◽  
pp. 7980-7985 ◽  
Author(s):  
Aleksandra M. Torbica ◽  
Dragan R. Živančev ◽  
Zorica T. Nikolić ◽  
Vuk B. Đorđević ◽  
Branislava G. Nikolovski
Keyword(s):  
Trypsin Inhibitor ◽  
Lab On A Chip ◽  
Kunitz Trypsin Inhibitor
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