Phase imaging of mechanical properties of live cells (Conference Presentation)

2017 ◽  
Author(s):  
Adam Wax
Keyword(s):  
Mechanical Properties ◽  
Live Cells ◽  
Phase Imaging

Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging

2016 ◽  
Vol 9 (435) ◽  
pp. rs5-rs5 ◽  
Author(s):  
K. Elsayad ◽  
S. Werner ◽  
M. Gallemi ◽  
J. Kong ◽  
E. R. Sanchez Guajardo ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Fluorescence Emission ◽  
Live Cells

AFM: The art of touching molecules

1994 ◽  
Vol 52 ◽  
pp. 1048-1049
Author(s):  
V. T. Moy ◽  
U. G. Hofmann ◽  
M. Benoit ◽  
D. Wagner ◽  
M. Ludwig ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Protein Complexes ◽  
Large Degree ◽  
Live Cells ◽  
Force Microscopy ◽  
Langmuir Blodgett ◽  
Atomic Force ◽  
Supramolecular Arrangements ◽  
Langmuir Blodgett Films ◽  
Broad Variety

During recent years the atomic force microscopy (AFM) has evolved into an extremely useful instrument in life sciences. Particularly the option of this novel technique to operate under quasi physiological conditions and in real time has initiated a broad spectrum of new experiments. As a result, structural and micro-mechanical properties of supramolecular arrangements like molecular films and protein complexes were elucidated. Dynamic processes in live cells were recorded at unparalleled resolution and molecular interactions were investigated. Selected examples of such aspects will be the topic of this lecture.The AFM has improved drastically the understanding of the molecular structure of Langmuir-Blodgett films. LB films have in turn evolved into standards for the improvement of the understanding of the fundamental imaging mechanisms because of several reasons: they may be formed from a broad variety of substances and their molecular packing can be varied and controlled to a large degree at the air water interface prior to transfer, allowing the intelligent design of certain surface properties like roughness and charge density or micro-mechanical properties.

scholarly journals Integrative quantitative-phase and airy light-sheet imaging

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
N. R. Subedi ◽  
P. S. Jung ◽  
E. L. Bredeweg ◽  
S. Nemati ◽  
S. E. Baker ◽  
...  
Keyword(s):  
Evolutionary Biology ◽  
Imaging System ◽  
Light Sheet ◽  
Live Cells ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Light Sheet Microscopy ◽  
Order Of Magnitude

AbstractLight-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.

scholarly journals Nanophotonics enhanced coverslip for phase imaging in biology

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Lukas Wesemann ◽  
Jon Rickett ◽  
Jingchao Song ◽  
Jieqiong Lou ◽  
Elizabeth Hinde ◽  
...  
Keyword(s):  
Human Cancer ◽  
Three Dimensional ◽  
Live Cells ◽  
Phase Imaging ◽  
Human Cancer Cells ◽  
Nanophotonic Devices ◽  
Optical Generation ◽  
Transparent Objects ◽  
Medical Diagnostic ◽  
Intensity Images

AbstractThe ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experimentally demonstrate a novel nanostructured coverslip that converts phase information to high-contrast intensity images. This compact device enables real-time, all-optical generation of pseudo three-dimensional images of phase objects on transmission. We show that by placing unstained human cancer cells on the device, the internal structure within the cells can be clearly seen. Our research demonstrates the significant potential of nanophotonic devices for integration into compact imaging and medical diagnostic devices.

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