Real-time and non-invasive quantitative phase imaging of pancreatic ductal adenocarcinoma cell mechanical properties

2019 ◽  
Author(s):  
Dawn Gillies ◽  
Wesam Gamal ◽  
Marta Canel ◽  
Yvonne Reinwald ◽  
Ying Yang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Real Time ◽  
Ductal Adenocarcinoma ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Adenocarcinoma Cell ◽  
Quantitative Phase ◽  
Non Invasive ◽  
Cell Mechanical Properties

scholarly journals Quantitative phase imaging of stromal prognostic markers in pancreatic ductal adenocarcinoma

2020 ◽  
Vol 11 (3) ◽  
pp. 1354 ◽  
Author(s):  
Michael Fanous ◽  
Adib Keikhosravi ◽  
Andre Kajdacsy-Balla ◽  
Kevin W. Eliceiri ◽  
Gabriel Popescu
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Prognostic Markers ◽  
Ductal Adenocarcinoma ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

scholarly journals Off-axis quantitative phase imaging processing using CUDA: toward real-time applications

2011 ◽  
Vol 2 (7) ◽  
pp. 1781 ◽  
Author(s):  
Hoa Pham ◽  
Huafeng Ding ◽  
Nahil Sobh ◽  
Minh Do ◽  
Sanjay Patel ◽  
...  
Keyword(s):  
Real Time ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Imaging Processing ◽  
Quantitative Phase ◽  
Real Time Applications

Quantitative phase imaging of cellular and subcellular structures for non-invasive screening diagnostics of socially significant diseases

2015 ◽  
Author(s):  
Irina Vasilenko ◽  
Vladislav Metelin ◽  
Marat Nasyrov ◽  
Vladimir Belyakov ◽  
Alexander Kuznetsov ◽  
...  
Keyword(s):  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Non Invasive ◽  
Screening Diagnostics

scholarly journals Real time blood testing using quantitative phase imaging

2013 ◽  
Author(s):  
Hoa V. Pham ◽  
Basanta Bhaduri ◽  
Krishnarao Tangella ◽  
Catherine Best-Popescu ◽  
Gabriel Popescu
Keyword(s):  
Real Time ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Blood Testing ◽  
Quantitative Phase

Real-time quantitative phase imaging in biomedicine

SPIE Newsroom ◽  
2013 ◽  
Author(s):  
Basanta Bhaduri ◽  
Hoa Pham ◽  
David Wickland ◽  
Gabriel Popescu
Keyword(s):  
Real Time ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

scholarly journals The potential and prospects of the quantitative phase imaging (QPI) diagnostic technology in obstetrics and gynecology

2018 ◽  
Vol 46 (8) ◽  
pp. 758-764
Author(s):  
R. A. Lifenko ◽  
O. S. Popova ◽  
A. G. Topuzov ◽  
V. D. Zverzhhovsky
Keyword(s):  
Uterine Leiomyoma ◽  
Interference Microscopy ◽  
Competent Cell ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Cytotoxic Potential ◽  
Quantitative Phase ◽  
Non Invasive ◽  
Phase Interference ◽  
Diagnostic Technology

Rationale:An important role of immunological factors in the endometriosis pathophysiology allows for their consideration as diagnostic and prognostic disease markers. A promising approach to the diagnosis of immunological abnormalities is based on the non-invasive cell diagnostic technology of quantitative phase imaging (QPI), with underlying principles of laser interferometry and holography.Aim:To assess the QPI potential to identify diagnostic criteria for cell immunity abnormalities in female patients with external endometriosis.Materials and methods:We performed a quantitative assessment of the cytotoxic potential of peripheral blood lymphocytes from 22 patients with endometrioid ovarian cysts (mean age, 27.1 ± 3.4 years), 20 patients with uterine leiomyoma (mean age, 29.3 ± 4.1 years) and 20 health non-pregnant women of child-bearing age (mean age, 28.7 ± 3.6 years). We used the non-invasive QPI technique with the module for phase interference microscopy of the Russian hardand software complex Bioni (Westtrade Ltd., Russia).Results:We studied phase interference pictures of native lymphocytes to identify morphological and densitometry characteristics for CD4+ и CD8+ cells. Two groups of cells were identified in the cytotoxic lymphocyte population, which differed in their values of the phase thickness (Hcyt) and the area of cytoplasm adjacent to the membrane and containing the perforin granules (Scyt). It was found, that in the healthy controls the proportion of perforin-positive cytotoxic CD8+ lymphocytes was 37.1 ± 5.15%, in the patients with endometriosis 29.8 ± 6.34%, and in those with uterine leiomyoma 42.6 ± 5.89%.Discussion:Assessment of the lymphocyte cytotoxic potential by quantitative determination of cells containing perforin granules may be an important diagnostic and prognostic criterion of the disease course.Conclusion:Studies of the intracellular structures of lymphocytes by non-invasive and non-reagent technology QPI allows not only for a detailed evaluation of pathophysiological mechanisms of immune competent cell transformation in the course of a pathological process, but also gives future directions for development of new approaches to functional assessment of the immune system based on the results obtained.

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