High resolution quantitative phase imaging of live cells with constrained optimization approach

2016 ◽  
Author(s):  
Vimal Prabhu Pandiyan ◽  
Kedar Khare ◽  
Renu John
Keyword(s):  
High Resolution ◽  
Constrained Optimization ◽  
Live Cells ◽  
Optimization Approach ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

scholarly journals Integrative quantitative-phase and airy light-sheet imaging

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
N. R. Subedi ◽  
P. S. Jung ◽  
E. L. Bredeweg ◽  
S. Nemati ◽  
S. E. Baker ◽  
...  
Keyword(s):  
Evolutionary Biology ◽  
Imaging System ◽  
Light Sheet ◽  
Live Cells ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Light Sheet Microscopy ◽  
Order Of Magnitude

AbstractLight-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.

scholarly journals High-resolution impedance mapping using electrically activated quantitative phase imaging

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Cristina Polonschii ◽  
Mihaela Gheorghiu ◽  
Sorin David ◽  
Szilveszter Gáspár ◽  
Sorin Melinte ◽  
...  
Keyword(s):  
High Resolution ◽  
Spatial Resolution ◽  
Indium Tin Oxide ◽  
Electrical Impedance ◽  
Unmet Need ◽  
Optical Path ◽  
Optical Methods ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

AbstractRetrieving electrical impedance maps at the nanoscale rapidly via nondestructive inspection with a high signal-to-noise ratio is an unmet need, likely to impact various applications from biomedicine to energy conversion. In this study, we develop a multimodal functional imaging instrument that is characterized by the dual capability of impedance mapping and phase quantitation, high spatial resolution, and low temporal noise. To achieve this, we advance a quantitative phase imaging system, referred to as epi-magnified image spatial spectrum microscopy combined with electrical actuation, to provide complementary maps of the optical path and electrical impedance. We demonstrate our system with high-resolution maps of optical path differences and electrical impedance variations that can distinguish nanosized, semi-transparent, structured coatings involving two materials with relatively similar electrical properties. We map heterogeneous interfaces corresponding to an indium tin oxide layer exposed by holes with diameters as small as ~550 nm in a titanium (dioxide) over-layer deposited on a glass support. We show that electrical modulation during the phase imaging of a macro-electrode is decisive for retrieving electrical impedance distributions with submicron spatial resolution and beyond the limitations of electrode-based technologies (surface or scanning technologies). The findings, which are substantiated by a theoretical model that fits the experimental data very well enable achieving electro-optical maps with high spatial and temporal resolutions. The virtues and limitations of the novel optoelectrochemical method that provides grounds for a wider range of electrically modulated optical methods for measuring the electric field locally are critically discussed.

scholarly journals Quantitative phase imaging of live cells using fast Fourier phase microscopy

2007 ◽  
Vol 46 (10) ◽  
pp. 1836 ◽  
Author(s):  
Niyom Lue ◽  
Wonshik Choi ◽  
Gabriel Popescu ◽  
Takahiro Ikeda ◽  
Ramachandra R. Dasari ◽  
...  
Keyword(s):  
Live Cells ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Phase Microscopy

High-resolution quantitative phase imaging based on a spatial light modulator and incremental binary random sampling

2020 ◽  
Vol 59 (20) ◽  
pp. 6148 ◽  
Author(s):  
Zhao Wang ◽  
Gong-Xiang Wei ◽  
Xiao-Lu Ge ◽  
Hui-Qiang Liu ◽  
Ben-Yi Wang
Keyword(s):  
High Resolution ◽  
Random Sampling ◽  
Spatial Light Modulator ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Light Modulator ◽  
Quantitative Phase

scholarly journals Holo-UNet: hologram-to-hologram neural network restoration for high fidelity low light quantitative phase imaging of live cells

2020 ◽  
Vol 11 (10) ◽  
pp. 5478
Author(s):  
Zhiduo Zhang ◽  
Yujie Zheng ◽  
Tienan Xu ◽  
Avinash Upadhya ◽  
Yean Jin Lim ◽  
...  
Keyword(s):  
Neural Network ◽  
Live Cells ◽  
High Fidelity ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Low Light ◽  
Quantitative Phase ◽  
Network Restoration
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