Correlative atomic force and confocal fluorescence microscopy: single molecule imaging and force induced spectral shifts (Conference Presentation)

2016 ◽  
Author(s):  
Thomas Basché ◽  
Gerald Hinze ◽  
Sven Stöttinger
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Confocal Fluorescence Microscopy ◽  
Single Molecule Imaging ◽  
Atomic Force ◽  
Spectral Shifts ◽  
Confocal Fluorescence

Measuring Diffusion in Nanoscale Environments with Single‐Molecule Confocal Fluorescence Microscopy

2004 ◽  
Vol 37 (2) ◽  
pp. 129-149 ◽  
Author(s):  
Daniel L. Burden ◽  
Peter K. Walhout ◽  
John T. Elliott ◽  
Emily L. Chandler ◽  
Roger G. Scharf ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence

Real-time observation of single molecules by confocal fluorescence microscopy

1995 ◽  
Vol 53 ◽  
pp. 60-61
Author(s):  
Shuming Nie ◽  
Daniel T. Chiu ◽  
Richard N. Zare
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Single Molecule ◽  
High Efficiency ◽  
Spherical Aberration ◽  
Correlation Spectroscopy ◽  
Optical Diffraction ◽  
Confocal Fluorescence Microscopy ◽  
Scanning Tunneling ◽  
Confocal Fluorescence

The ability to detect, identify, and manipulate individual molecules offer exciting possibilities in many fields, including chemical analysis, materials research, and the biological sciences. A particularly powerful approach is to combine the exquisite sensitivity of laser-induced fluorescence and the spatial localization and imaging capabilities of diffraction-limited or near-field optical microscopes. Unlike scanning tunneling microscopy (STM) and atomic force microscopy (AFM), which lack molecular specificity, optical spectroscopy and microscopy techniques can be used for real-time monitoring and molecular identification at nanometer dimensions or in ultrasmall volumes.We report the use of confocal fluorescence microscopy coupled with a diffraction-limit laser beam and a high-efficiency photodiode for real-time detection of single fluorescent molecules in solution at room temperature. Rigler and Eigen have also demonstrated single-molecule detection with a confocal microscope and fluorescence correlation spectroscopy. The probe (or sampling) volume is effectively an elongated cylinder, with its radius being determined by optical diffraction and length by spherical aberration.

scholarly journals Photoinduced Electron Transfer of Oxazine 1/TiO2Nanoparticles at Single Molecule Level by Using Confocal Fluorescence Microscopy

Langmuir ◽  
2010 ◽  
Vol 26 (11) ◽  
pp. 9050-9060 ◽  
Author(s):  
Yi-Ju Chen ◽  
Hsin-Yu Tzeng ◽  
Hsiu-Fang Fan ◽  
Ming-Shiang Chen ◽  
Jer-Shing Huang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Confocal Fluorescence Microscopy ◽  
Single Molecule Level ◽  
Confocal Fluorescence ◽  
Oxazine 1

Morphology of protein polymer hybrid films studied by atomic force microscopy and scanning confocal fluorescence microscopy

2003 ◽  
Vol 443 (1-2) ◽  
pp. 124-135 ◽  
Author(s):  
Philippe Foubert ◽  
Jurry M. Hannink ◽  
Fabian Köhn ◽  
Roel Gronheid ◽  
Nico A.J.M. Sommerdijk ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Hybrid Films ◽  
Force Microscopy ◽  
Protein Polymer ◽  
Polymer Hybrid ◽  
Atomic Force ◽  
Confocal Fluorescence
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