scholarly journals Photoinduced Electron Transfer of Oxazine 1/TiO2Nanoparticles at Single Molecule Level by Using Confocal Fluorescence Microscopy

Langmuir ◽  
2010 ◽  
Vol 26 (11) ◽  
pp. 9050-9060 ◽  
Author(s):  
Yi-Ju Chen ◽  
Hsin-Yu Tzeng ◽  
Hsiu-Fang Fan ◽  
Ming-Shiang Chen ◽  
Jer-Shing Huang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Confocal Fluorescence Microscopy ◽  
Single Molecule Level ◽  
Confocal Fluorescence ◽  
Oxazine 1

Measuring Diffusion in Nanoscale Environments with Single‐Molecule Confocal Fluorescence Microscopy

2004 ◽  
Vol 37 (2) ◽  
pp. 129-149 ◽  
Author(s):  
Daniel L. Burden ◽  
Peter K. Walhout ◽  
John T. Elliott ◽  
Emily L. Chandler ◽  
Roger G. Scharf ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence

scholarly journals Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jonathan Schubert ◽  
Andrea Schulze ◽  
Chrisostomos Prodromou ◽  
Hannes Neuweiler
Keyword(s):  
Electron Transfer ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Conformational Changes ◽  
Photoinduced Electron Transfer ◽  
Structural Changes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Imaging Microscopy ◽  
Pet Probes

AbstractMany proteins are molecular machines, whose function is dependent on multiple conformational changes that are initiated and tightly controlled through biochemical stimuli. Their mechanistic understanding calls for spectroscopy that can probe simultaneously such structural coordinates. Here we present two-colour fluorescence microscopy in combination with photoinduced electron transfer (PET) probes as a method that simultaneously detects two structural coordinates in single protein molecules, one colour per coordinate. This contrasts with the commonly applied resonance energy transfer (FRET) technique that requires two colours per coordinate. We demonstrate the technique by directly and simultaneously observing three critical structural changes within the Hsp90 molecular chaperone machinery. Our results reveal synchronicity of conformational motions at remote sites during ATPase-driven closure of the Hsp90 molecular clamp, providing evidence for a cooperativity mechanism in the chaperone’s catalytic cycle. Single-molecule PET fluorescence microscopy opens up avenues in the multi-dimensional exploration of protein dynamics and allosteric mechanisms.

Photoinduced electron transfer (PET)-probes for the study of enzyme activity at the ensemble and single molecule level

2007 ◽  
Author(s):  
Sigrun Henkenjohann ◽  
Sebastian van de Linde ◽  
Sören Doose ◽  
Philip Tinnefeld ◽  
Markus Sauer
Keyword(s):  
Electron Transfer ◽  
Enzyme Activity ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Single Molecule Level ◽  
Pet Probes

Real-time observation of single molecules by confocal fluorescence microscopy

1995 ◽  
Vol 53 ◽  
pp. 60-61
Author(s):  
Shuming Nie ◽  
Daniel T. Chiu ◽  
Richard N. Zare
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Single Molecule ◽  
High Efficiency ◽  
Spherical Aberration ◽  
Correlation Spectroscopy ◽  
Optical Diffraction ◽  
Confocal Fluorescence Microscopy ◽  
Scanning Tunneling ◽  
Confocal Fluorescence

The ability to detect, identify, and manipulate individual molecules offer exciting possibilities in many fields, including chemical analysis, materials research, and the biological sciences. A particularly powerful approach is to combine the exquisite sensitivity of laser-induced fluorescence and the spatial localization and imaging capabilities of diffraction-limited or near-field optical microscopes. Unlike scanning tunneling microscopy (STM) and atomic force microscopy (AFM), which lack molecular specificity, optical spectroscopy and microscopy techniques can be used for real-time monitoring and molecular identification at nanometer dimensions or in ultrasmall volumes.We report the use of confocal fluorescence microscopy coupled with a diffraction-limit laser beam and a high-efficiency photodiode for real-time detection of single fluorescent molecules in solution at room temperature. Rigler and Eigen have also demonstrated single-molecule detection with a confocal microscope and fluorescence correlation spectroscopy. The probe (or sampling) volume is effectively an elongated cylinder, with its radius being determined by optical diffraction and length by spherical aberration.

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