Imaging of mucus clearance in the airways of living spontaneously breathing mice by optical coherence microscopy (Conference Presentation)

2016 ◽  
Author(s):  
Mario Pieper ◽  
Hinnerk Schulz-Hildebrandt ◽  
Gereon Hüttmann ◽  
Peter König
Keyword(s):  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Mucus Clearance ◽  
Spontaneously Breathing

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

Peripheral mucociliary clearance with high-frequency chest wall compression

1985 ◽  
Vol 58 (4) ◽  
pp. 1157-1163 ◽  
Author(s):  
D. Gross ◽  
A. Zidulka ◽  
C. O'Brien ◽  
D. Wight ◽  
R. Fraser ◽  
...  
Keyword(s):  
Phase I ◽  
Chest Wall ◽  
High Frequency ◽  
Cuff Pressure ◽  
Region Of Interest ◽  
Peripheral Region ◽  
Phase Iii ◽  
Mucus Clearance ◽  
Spontaneously Breathing ◽  
Tracheal Mucus

We investigated the effects of high-frequency chest wall compression (HFCWC) on peripheral and tracheal mucus clearance in anesthetized spontaneously breathing dogs. HFCWC was achieved by oscillating the pressure in a thoracic cuff with a piston pump. Regional lung retention of a technetium-99m sulfur colloid aerosol was monitored with a gamma camera. A peripheral mucus clearance index (PMCI) was defined for each region of interest. The tracheal mucus clearance rate (TMCR) was determined by bronchoscopic visualization of marker particle transport. Phase I: In seven dogs, 30 min of HFCWC at 13 Hz with peak cuff pressure (Pcuff) 100–120 cmH2O was found to significantly enhance PMCI in regions immediately under the cuff. (delta PMCI = 24.4 +/- 4.6 in the basal peripheral region.) Phase II: Because of subpleural hemorrhage in phase I, the effect of HFCWC on TMCR at various Pcuff levels was studied in five dogs. The enhancement of TMCR by HFCWC reached a plateau level at Pcuff = 50 cmH2O. Phase III: HFCWC at 13 Hz with Pcuff = 50–60 cmH2O was found to significantly enhance PMCI in five dogs without the consequence of hemorrhage. Correlations were found between the enhancement of PMCI and TMCR by HFCWC. These results demonstrate that HFCWC is effective in enhancing both peripheral and central mucus clearance in dogs and safe when moderate pressures are applied.

A study of skin deformation by optical coherence microscopy

BIOPHYSICS ◽  
2010 ◽  
Vol 55 (2) ◽  
pp. 301-304 ◽  
Author(s):  
A. V. Zeveke ◽  
D. V. Shabanov
Keyword(s):  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Skin Deformation

scholarly journals In vivo imaging of corneal nerves and cellular structures in mice with Gabor-domain optical coherence microscopy

2020 ◽  
Vol 11 (2) ◽  
pp. 711 ◽  
Author(s):  
Cristina Canavesi ◽  
Andrea Cogliati ◽  
Amanda Mietus ◽  
Yue Qi ◽  
Jesse Schallek ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Cellular Structures ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Corneal Nerves ◽  
Gabor Domain
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