Histological study of subcutaneous fat at NIR laser treatment of the rat skinin vivo

2015 ◽  
Author(s):  
I. Y. Yanina ◽  
Yu. I. Svenskaya ◽  
N. A. Navolokin ◽  
O. V. Matveeva ◽  
A. B. Bucharskaya ◽  
...  
Keyword(s):  
Laser Treatment ◽  
Histological Study ◽  
Subcutaneous Fat ◽  
Nir Laser

Fat tissue histological study at NIR laser treatment of the skin in vivo

2011 ◽  
Author(s):  
Irina Y. Yanina ◽  
Valery V. Tuchin ◽  
Nikita A. Navolokin ◽  
Olga V. Matveeva ◽  
Alla B. Bucharskaya ◽  
...  
Keyword(s):  
Laser Treatment ◽  
Histological Study ◽  
Fat Tissue ◽  
Nir Laser

Fat tissue histological study at NIR laser treatment of the skin in vivo

2011 ◽  
Author(s):  
Irina Yu. Yanina ◽  
Valery V. Tuchin ◽  
Nikita A. Navolokin ◽  
Olga V. Matveeva ◽  
Alla B. Bucharskaya ◽  
...  
Keyword(s):  
Laser Treatment ◽  
Histological Study ◽  
Fat Tissue ◽  
Nir Laser

scholarly journals Factors that could influence the severity of post-traumatic lung fat embolism - a prospective histological study -

2003 ◽  
Vol 131 (5-6) ◽  
pp. 244-248 ◽  
Author(s):  
Slobodan Nikolic ◽  
Jelena Micic ◽  
Slobodan Savic ◽  
Milan Gajic
Keyword(s):  
Cause Of Death ◽  
Histological Study ◽  
Subcutaneous Fat ◽  
Hypovolemic Shock ◽  
Fat Embolism ◽  
Older Individuals ◽  
Fat Globules ◽  
Sudan Iii ◽  
Post Traumatic ◽  
Severe Grade

INTRODUCTION Each fracture of long or pelvic bones as well as large contusions of subcutaneous fat tissue cause releasing of fat globules that rapidly penetrate into circulation through the ruptured veins of the injured tissue, and reach the lung circulation [1,2]. During the first phase, fat emboli block the functional lung circulation by their mechanical effect in capillaries producing so called isolated post-traumatic lung fat embolism [3]. The surface layer of a fat embolus, which is practically in liquid state, behaves as a membrane of very high density, i.e., as it is under high pressure which obstruct the blood stream [4] that is finally stopped at the level of lung blood vessels with diameter of approximately 20 ? [5].This pathophysiological mechanism produces cor pulmonale acutum, with poor pathological findings [8]. Nowadays, the post-mortem diagnosis of lung fat embolism is based on microscopical examination of tissue specimens, usually prepared with special histological staining (Sudan III) [9]. The grading of fat embolism according to Sevitt's criteria is generally accepted [10]. Taking of slices from apicoventral areas of the lungs has been recommended [11]. With longer outliving period, the total number of fat emboli in the lung circulation gradually decreases, due to their disintegration and resorption. It has been stated that fat globules completely disappear about 4-6 weeks after injury, and that they should not be searched for microscopically in this post-traumatic phase [11]. OBJECTIVES The aim of our work was to determine whether the age of injured, their gender, total severity of trauma, outliving period, and hypovole-mic shock that develops after injuring, may induce development of more severe forms of post-traumatic lung fat embolism. MATERIAL AND METHODS A prospective histological study was performed on the autopsy material of the Institute of Forensic Medicine in Belgrade. The analyzed sample consisted of individuals with injuries that might be a source of fat emboli (fractures of long bones, large contusions of subcutaneous fat tissue). The lung slices were systematically taken and stained with special fat staining (Sudan III). In each particular case, the grade of lung fat embolism was counted on the basis of microscopical appearance, according to Sevitt's criteria. The total severity of trauma was estimated by calculation of the Injury Severity Score (ISS) [13,14]. In no cases from the analyzed sample, the fat embolism was mentioned as either singular or plural cause of death. The obtained results were analyzed by means of appropriates statistical methods (ANOVA LSD-test, x2 test, Man-Whitney test, Fischer's test of correct probability). RESULTS AND DISCUSSION The analyzed sample included 58 fatally injured individuals, 39 males and 19 females. The average age was 54.10 years (SD=16.56), the average value of ISS was 34.69 (SD=5.88), and the average outliving period was 3.74 days (SD=5.88). However, all these data look differently when the analyzed sample has been stratified and analyzed according to the estimated grade of lung fat embolism. It was not showed that severity of lung fat embolism depends on sex of the injured (^2=0.842 p>0.05). The groups with the slightest and the most severe grade of lung fat embolism are statistically significantly different in relation to age of individuals (ANOVA, p=0.017). By means of LSD test, it has been showed that the group with the most severe grade of lung fat embolism (grade III) is statistically significantly different comparing to other two groups (with grade I and II) in relation to the age of injured (the values are p=0.16 and p=0.19 respectively, and the both groups are less than p=0.05). In the group with the most severe grade of lung fat embolism, the older individuals are statistically significantly represented comparing to othertwo groups. CONCLUSION The analysis of our sample showed that the most severe grade of post-traumatic lung fat embolism (microscopical grade III according to Sevitt's criteria) was determined in older individuals, more severely injured, and with shorter outliving period. The severity of fat embolism depends neither on sex of the injured, nor on development of post-traumatic hypovolemic shock. The obtained results related to the influence of hypovolemic shock on severity of fat embolism should be accepted with a caution. Namely, sometimes there is an intention to simplify a procedure of creating of autopsy conclusion about the cause of death, so that loss of blood is not mentioned at all, in spite of fact that it could have been a concurrent cause of death, while in other cases exsanguination is designated as a sole cause of death, forgetting the possibility that fat embolism could have really been the immediate cause of death.

Transluminal laser treatment with metal-capped optical fibres of normal coronary arteries in live dogs: an angiographic and histological study of the acute effects

1987 ◽  
Vol 2 (3) ◽  
pp. 159-163 ◽  
Author(s):  
Filippo Crea ◽  
Graham J. Davies ◽  
William J McKenna ◽  
Mehdi Pashazadeh ◽  
Sally P Allwork ◽  
...  
Keyword(s):  
Laser Treatment ◽  
Coronary Arteries ◽  
Histological Study ◽  
Optical Fibres ◽  
Acute Effects ◽  
Normal Coronary Arteries

scholarly journals Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green

2017 ◽  
Vol 22 (5) ◽  
pp. 055008 ◽  
Author(s):  
Irina Yu. Yanina ◽  
Nikita A. Navolokin ◽  
Yulia I. Svenskaya ◽  
Alla B. Bucharskaya ◽  
Galina N. Maslyakova ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Laser Irradiation ◽  
Subcutaneous Fat ◽  
Nir Laser

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

2019 ◽  
Vol 12 (12) ◽  
Author(s):  
Irina Y. Yanina ◽  
Nikita A. Navolokin ◽  
Alla B. Bucharskaya ◽  
Galina N. Мaslyakova ◽  
Valery V. Tuchin
Keyword(s):  
Laser Treatment ◽  
Subcutaneous Fat

scholarly journals NIR Laser-Responsive PNIPAM and Gold Nanorod Composites for the Engineering of Thermally Reactive Drug Delivery Nanomedicine

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 204 ◽  
Author(s):  
Yejin Kwon ◽  
Yonghyun Choi ◽  
Jaehee Jang ◽  
Semi Yoon ◽  
Jonghoon Choi
Keyword(s):  
Drug Release ◽  
Laser Treatment ◽  
Near Infrared ◽  
Raft Polymerization ◽  
The Body ◽  
Solution Temperature ◽  
Future Research ◽  
Microscopy Imaging ◽  
Binding Rate ◽  
Nir Laser

When ingesting a drug on its own or injecting it directly into tissue, its concentration increases immediately within the body, which often exacerbates the side effects and increases its toxicity. To solve this problem, we synthesized the thermally reactive polymer poly(N-isopropylacrylamide) (PNIPAM) using reversible addition–fragmentation chain transfer (RAFT) polymerization and prepared nanocarriers by binding PNIPAM to gold nanorods (GRs), with the anticancer agent doxorubicin (DOX) used as a model drug. PNIPAM changes from hydrophilic to hydrophobic at temperatures above its lower critical solution temperature, which represents a coil-to-globule volume phase transition. Because GRs absorb near-infrared (NIR) laser light and emit energy, PNIPAM aggregation occurs when the synthesized PNIPAM/GR are subjected to an NIR laser, and the temperature of the GRs rises. Using this principle, DOX was combined with the PNIPAM/GR complex, and the resulting anticancer effects with and without laser treatment were observed in Hela and MDA-MB-231 cells. In our proposed complex, the GR binding rate of PNIPAM reached 20% and the DOX binding rate reached 15%. The release profile of the drug following laser irradiation was determined using a drug release test and confocal microscopy imaging. It was subsequently confirmed that the release of the drug is higher at higher temperatures, especially with laser treatment. The proposed combination of temperature-reactive polymers and gold nanostructures shows promise for future research into controlled drug release.

Effects of a single near-infrared laser treatment on cutaneous wound healing: Biometrical and histological study in rats

2007 ◽  
Vol 87 (3) ◽  
pp. 145-153 ◽  
Author(s):  
Sandra B. Rezende ◽  
Martha S. Ribeiro ◽  
Silvia C. Núñez ◽  
Valdir G. Garcia ◽  
Edison P. Maldonado
Keyword(s):  
Wound Healing ◽  
Laser Treatment ◽  
Near Infrared ◽  
Histological Study ◽  
Infrared Laser ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

NIR Laser‐Treatment, Anti‐Oxidation Upconversion Nanoparticles for Optical Temperature Sensing

2021 ◽  
Vol 6 (38) ◽  
pp. 10263-10273
Author(s):  
Yandong Bai ◽  
Yuemei Li ◽  
Rui Wang ◽  
Yongmei Li
Keyword(s):  
Laser Treatment ◽  
Temperature Sensing ◽  
Upconversion Nanoparticles ◽  
Nir Laser

scholarly journals STRUCTURE OF THE PERIANAL REGION IN CHILDREN WITH ANORECTAL MALFORMATIONS

2018 ◽  
Vol 17 (2) ◽  
pp. 42-49
Author(s):  
V. S. Konoplitsʹkyy ◽  
O. O. Lukiyanetsʹ ◽  
S. O. Sokolʹnyk
Keyword(s):  
Anal Sphincter ◽  
External Anal Sphincter ◽  
Striated Muscle ◽  
Histological Study ◽  
Subcutaneous Fat ◽  
Anorectal Malformations ◽  
Apical Part ◽  
Structural Elements ◽  
Perianal Region ◽  
Developmental Defects

Objective: To study the morphological factors in the structure of the abdominal part of the colon and perineum in anorectal malformations in children. Material and methods: In 10 patients (1 day-2, 5 years) with anorectal developmental defects, a complex histological study of myo- and neurostructures of abdominal fragments from the rectum was performed, which were removed during surgery, with proctoplasty for the correction of the congenital pathology of the anorectal region. The study group consisted of 2 patients with unsupported forms of vice, 7 patients with fistula in the urinary system and one child with fistula per perineum. Results of the study. The search for the study was focused on revealing the fibers of the transversely striated muscle tissue of the subcutaneous part of the external anal sphincter, as one of the factors of its structure (presence). It was determined that the structural elements of the structure of the perianal region and pelvic floor in children with anorectal defects have certain morphological features, namely: the skin and subcutaneous fat, at the location of the necessary location of the external anus, in 80% of cases did not contain the muscular elements of the surface part of the external anal sphincter; the wall of the apical part of the rectum was characterized by hypoplasia of the mucosa and in most cases of the muscle layer with the fullness of their vessels and the presence of autonomic intermuscular ganglia. Conclusion. The structural elements of perianal area and pelvic bottom anatomy of children with аnorectal development defestes have morphological peculiarities are certain, namely: in the place of needed location of external anal hole the skin and hypodermic-fatty cellular had not contain the muscular elements of superficial part external anal sphincter at 80% cases; the wall of apical part of rectum characterized by the hypoplasia of mucous membrane and the muscular layer with the plethora of their vessels in most cases and presence of vegetative intermuscular neuroganglions.

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