Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

2019 ◽  
Vol 12 (12) ◽  
Author(s):  
Irina Y. Yanina ◽  
Nikita A. Navolokin ◽  
Alla B. Bucharskaya ◽  
Galina N. Мaslyakova ◽  
Valery V. Tuchin
Keyword(s):  
Laser Treatment ◽  
Subcutaneous Fat

scholarly journals POS1388 ULTRASOUND FOR PANNICULITIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 977.1-977
Author(s):  
A. Potapova ◽  
O. Egorova ◽  
O. Alekseeva ◽  
A. Volkov ◽  
S. Radenska-Lopovok
Keyword(s):  
Dynamic Range ◽  
Subcutaneous Fat ◽  
Diagnostic Value ◽  
High Energy ◽  
Contralateral Side ◽  
Imaging Method ◽  
Non Invasive ◽  
M 12

Background:Ultrasound (US) is a non-invasive and safe imaging method that allows in vivo differentiation of the morphological structures of subcutaneous fat (SCF) tissue in in normal and pathology.Objectives:Reveal features of ultrasound changes in SCF in panniculitis (Pn).Methods:57 patients (f – 45, m - 12) aged 18 - 67 years with an initial diagnosis of erythema nodosum and a disease duration of 3.6 ± 1.4 years were examined. In addition to the general clinical examination, a computed tomography of the chest organs and a pathomorphological examination of a skin biopsy from the site of the node were performed. Ultrasound was performed on a MyLabTwice apparatus (ESAOTE, Italy) using a multi-frequency linear transducer (10-18 MHz) with the PD technique, the parameters of which were adapted for recording low-speed flows (PRF 300-600 Hz, low filter, dynamic range - 20-40 dB), the presence of vascularization was assessed not only in the affected area, but also on the contralateral side using high-energy Doppler.Results:33 patients were diagnosed with septal Pn (SPn), 24 - lobular Pn (LPn). In all cases, the diagnosis was verified by histological examination. Ultrasound made it possible to assess the thickness, echoicity and vascularization of the SCF. In 35 patients, significant thickening of the SCF was revealed (as compared to the contralateral side), of which in 14 cases with SPn, in 21 - with LPn. Significant diffuse thickening of the SCF with the contralateral side was observed in 18 patients, incl. in 12 (66%) patients with LPn. Limited thickening was more typical for SPn (73%). A significant increase in the echoicity of the SCF was noted in all forms of Pn. A “lobular” echo pattern with an anechogenic environment was observed in 25 patients, of which 18 (72%) had LPn. An increase in vascularization compared to the contralateral side was recorded in 30 cases (SPn-17, LPn-13).Conclusion:The obtained preliminary results indicate the important role of ultrasound in assessing the depth and prevalence of the inflammatory process at Pn. To clarify the diagnostic value of this method, further studies are needed on a larger sample of patients.Disclosure of Interests:None declared

Using real-time ultrasound for in vivo assessment of carcass and internal adipose depots of dairy sheep

2019 ◽  
Vol 157 (7-8) ◽  
pp. 650-658
Author(s):  
J. Afonso ◽  
C. M. Guedes ◽  
A. Teixeira ◽  
V. Santos ◽  
J. M. T. Azevedo ◽  
...  
Keyword(s):  
Real Time ◽  
Prediction Models ◽  
Linear Regression Analysis ◽  
Subcutaneous Fat ◽  
Live Weight ◽  
Multiple Linear Regression Analysis ◽  
Fat Content ◽  
Fat Reserves ◽  
Intermuscular Fat

AbstractFifty-one Churra da Terra Quente ewes (4–7 years old) were used to analyse the potential of real-time ultrasound (RTU) to predict the amount of internal adipose depots, in addition to carcass fat (CF). The prediction models were developed from live weight (LW) and RTU measurements taken at eight different locations. After correlation and multiple linear regression analysis, the prediction models were evaluated by k-fold cross-validation and through the ratio of prediction to deviation (RPD). All prediction models included at least one RTU measurement as an independent variable. Prediction models for the absolute weight of the different adipose depots showed higher accuracy than prediction models for fat content per kg of LW. The former showed to be very good or excellent (2.4 ⩽ RPD ⩽ 3.8) for all adipose depots except mesenteric fat (MesF) and thoracic fat, with the model for MesF still providing useful information (RPD = 1.8). Prediction models for fat content per kg of LW were also very good or excellent for subcutaneous fat, intermuscular fat, CF and body fat (2.6 ⩽ RPD ⩽ 3.2), while the best prediction models for omental fat, kidney knob, channel fat and internal fat still provided useful information. Despite some loss in the accuracy of the estimates obtained, there was a similar pattern in terms of RPD for models developed from LW and RTU measurements taken just at the level of the 11th thoracic vertebra. In vivo RTU measurements showed the potential to monitor changes in ewe internal fat reserves as well as in CF.

scholarly journals Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use

2020 ◽  
Vol 10 (16) ◽  
pp. 5473
Author(s):  
Roman Matějka ◽  
Miroslav Koňařík ◽  
Jana Štěpanovská ◽  
Jan Lipenský ◽  
Jaroslav Chlupáč ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Stromal Cells ◽  
Subcutaneous Fat ◽  
Adipose Derived Stromal Cells ◽  
Pulsatile Pressure ◽  
Dynamic Cultivation ◽  
Decellularized Tissue ◽  
Static Conditions

(1) Background: Decellularized xenogeneic tissues are promising matrices for developing tissue-engineered cardiovascular grafts. In vitro recellularization of these tissues with stromal cells can provide a better in vivo remodelling and a lower thrombogenicity of the graft. The process of recellularization can be accelerated using a cultivation bioreactor simulating physiological conditions and stimuli. (2) Methods: Porcine pericardium was decellularized using a custom-built decellularization system with an optimized protocol. Autologous porcine adipose-derived stromal cells (PrASCs), isolated from the subcutaneous fat tissue, were used for recellularizing the decellularized pericardium. A custom cultivation bioreactor allowing the fixing of the decellularized tissue into a special cultivation chamber was created. The bioreactor maintained micro-perfusion and pulsatile pressure stimulation in order to promote the ingrowth of PrASCs inside the tissue and their differentiation. (3) Results: The dynamic cultivation promoted the ingrowth of cells into the decellularized tissue. Under static conditions, the cells penetrated only to the depth of 50 µm, whereas under dynamic conditions, the tissue was colonized up to 250 µm. The dynamic cultivation also supported the cell differentiation towards smooth muscle cells (SMCs). In order to ensure homogeneous cell colonization of the decellularized matrices, the bioreactor was designed to allow seeding of the cells from both sides of the tissue prior to the stimulation. In this case, the decellularized tissue was recolonized with cells within 5 days of dynamic cultivation. (4) Conclusions: Our newly designed dynamic bioreactor markedly accelerated the colonization of decellularized pericardium with ASCs and cell differentiation towards the SMC phenotype.

In vivo quantification of the structural abnormalities in psoriatic microvessels before and after pulsed dye laser treatment

2005 ◽  
Vol 152 (3) ◽  
pp. 505-511 ◽  
Author(s):  
S. Hern ◽  
A.W.B. Stanton ◽  
R.H. Mellor ◽  
C.C. Harland ◽  
J.R. Levick ◽  
...  
Keyword(s):  
Laser Treatment ◽  
Dye Laser ◽  
Pulsed Dye Laser ◽  
Structural Abnormalities ◽  
Before And After

scholarly journals Quantitative Monitoring of Tattoo Contrast Variations after 755-nm Laser Treatments in In Vivo Tattoo Models

Sensors ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 285
Author(s):  
Myeongjin Kim ◽  
Suhyun Park ◽  
Hyun Uk Lee ◽  
Hyun Wook Kang
Keyword(s):  
Laser Treatment ◽  
Quantitative Imaging ◽  
Imaging Method ◽  
Sprague Dawley ◽  
Scoring Method ◽  
Monitoring Method ◽  
Tattoo Removal ◽  
Quantitative Monitoring ◽  
Laser Treatments

Laser lights have been used by dermatologists for tattoo removal through photothermal interactions. However, most clinical studies used a visual scoring method to evaluate the tattoo removal process less objectively, leading to unnecessary treatments. This study aimed to develop a simple and quantitative imaging method to monitor the degree of tattoo removal in in vivo skin models. Sprague Dawley rat models were tattooed with four different concentrations of black inks. Laser treatment was performed weekly on the tattoos using a wavelength of 755 nm over six weeks. Images of non-treated and treated samples were captured using the same method after each treatment. The intensities of the tattoos were measured to estimate the contrast for quantitative comparison. The results demonstrated that the proposed monitoring method quantified the variations in tattoo contrast after the laser treatment. Histological analysis validated the significant removal of tattoo inks, no thermal injury to adjacent tissue, and uniform remodeling of epidermal and dermal layers after multiple treatments. This study demonstrated the potential of the quantitative monitoring technique in assessing the degree of clearance level objectively during laser treatments in clinics.

Picosecond‐Domain Fractional Laser Treatment Over Hyaluronic Acid Fillers: In Vivo and Clinical Studies

2020 ◽  
Vol 52 (10) ◽  
pp. 928-934
Author(s):  
Jung E. Kim ◽  
Jeong Y. Hong ◽  
Ho J. Lee ◽  
Sung Y. Lee ◽  
Hyun J. Kim
Keyword(s):  
Hyaluronic Acid ◽  
Laser Treatment ◽  
Clinical Studies ◽  
Fractional Laser

Comparison of seven ultrasonic techniques for in vivo estimation of beef carcass composition with special reference to performance testing

1990 ◽  
Vol 51 (3) ◽  
pp. 489-495 ◽  
Author(s):  
S. J. Porter ◽  
M. G. Owen ◽  
S. J. Page ◽  
A. V. Fisher
Keyword(s):  
Subcutaneous Fat ◽  
Performance Testing ◽  
Live Weight ◽  
Carcass Composition ◽  
Time Interval ◽  
Velocity Of Sound ◽  
Subjective Assessments ◽  
Intermuscular Fat ◽  
Ultrasonic Techniques

ABSTRACTForty-nine bulls, 27 Limousin × Friesian and 22 Charolais × Friesian, were evaluated and slaughtered in four batches of about equal size over 4 weeks. Each batch was of one breed. Age, live weight at evaluation and subjective assessments of fatness and conformation were recorded together with fat and muscle measurements by the Delphi, Meritronics, Scanogram, Vetscan, Kaijo Denki, Warren and the Velocity of Sound ultrasonic machines. Experienced operators were used to assess the performance of machine/operator combinations likely to be achieved in bull performance testing in the field. Fat thicknesses and areas, and m. longissimus areas were taken at the 10th rib and 13th rib, and 3rd lumbar regions by most machines. For the Delphi and Meritronics machines, fat thicknesses only were taken; for the Velocity of Sound machine, time interval measurements and anatomical distances were taken at the shoulder, mid back, lumbar and hind limb regions. The left side of each carcass was fully separated into lean, subcutaneous fat, intermuscular fat, bone and waste. All measurements were examined as potential predictors of carcass composition in step-wise regression in a model which included week of evaluation, breed and live weight at evaluation as the first independent variable. On the whole, scanning machines had a higher precision than A-mode machines, with the Velocity of Sound machine achieving the highest precision for carcass lean (g/kg) (residual s.d. = 13·0) and fat (g/kg) (residual s.d. = 14·1). None of the linear and area measurements taken on the carcass achieved the degree of precision of the Velocity of Sound, Scanogram and Vetscan machines.

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