Femtosecond optical injection of intact plant cells using a reconfigurable platform

2014 ◽  
Author(s):  
Claire A. Mitchell ◽  
Stefan Kalies ◽  
Tomas Cizmar ◽  
Nicola Bellini ◽  
Anisha Kubasik-Thayil ◽  
...  
Keyword(s):  
Plant Cells ◽  
Optical Injection ◽  
Intact Plant ◽  
Reconfigurable Platform

scholarly journals Carbon nanocarriers deliver siRNA to intact plant cells for efficient gene knockdown

2020 ◽  
Vol 6 (26) ◽  
pp. eaaz0495 ◽  
Author(s):  
Gozde S. Demirer ◽  
Huan Zhang ◽  
Natalie S. Goh ◽  
Rebecca L. Pinals ◽  
Roger Chang ◽  
...  
Keyword(s):  
Sirna Delivery ◽  
Control Plant ◽  
Plant Cells ◽  
Plant Biotechnology ◽  
Intact Plant ◽  
Intact Cells ◽  
Efficient Gene ◽  
Dna Vectors ◽  
And Control ◽  
Interfering Rna

Posttranscriptional gene silencing (PTGS) is a powerful tool to understand and control plant metabolic pathways, which is central to plant biotechnology. PTGS is commonly accomplished through delivery of small interfering RNA (siRNA) into cells. Standard plant siRNA delivery methods (Agrobacterium and viruses) involve coding siRNA into DNA vectors and are only tractable for certain plant species. Here, we develop a nanotube-based platform for direct delivery of siRNA and show high silencing efficiency in intact plant cells. We demonstrate that nanotubes successfully deliver siRNA and silence endogenous genes, owing to effective intracellular delivery and nanotube-induced protection of siRNA from nuclease degradation. This study establishes that nanotubes could enable a myriad of plant biotechnology applications that rely on RNA delivery to intact cells.

Accommodation of powdery mildew fungi in intact plant cells

2008 ◽  
Vol 165 (1) ◽  
pp. 5-18 ◽  
Author(s):  
Ruth Eichmann ◽  
Ralph Hückelhoven
Keyword(s):  
Powdery Mildew ◽  
Plant Cells ◽  
Intact Plant ◽  
Powdery Mildew Fungi

scholarly journals Photoinjection of fluorescent nanoparticles into intact plant cells using femtosecond laser amplifier

APL Photonics ◽  
2020 ◽  
Vol 5 (6) ◽  
pp. 066104
Author(s):  
Taufiq Indra Rukmana ◽  
Gabriela Moran ◽  
Rachel Méallet-Renault ◽  
Gilles Clavier ◽  
Tadashi Kunieda ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Plant Cells ◽  
Intact Plant ◽  
Fluorescent Nanoparticles ◽  
Laser Amplifier

scholarly journals Nanotubes Effectively Deliver siRNA to Intact Plant Cells and Protect siRNA Against Nuclease Degradation

2019 ◽  
Author(s):  
Gozde Demirer ◽  
Huan Zhang ◽  
Natalie Goh ◽  
Roger Chang ◽  
Markita Landry
Keyword(s):  
Plant Cells ◽  
Intact Plant ◽  
Nuclease Degradation

scholarly journals Pontentiometric Cyanine Dyes Are Sensitive Probes for Mitochondria in Intact Plant Cells

1987 ◽  
Vol 84 (4) ◽  
pp. 1385-1390 ◽  
Author(s):  
Zhanjiang Liu ◽  
W. R. Bushnell ◽  
Robert Brambl
Keyword(s):  
Plant Cells ◽  
Cyanine Dyes ◽  
Intact Plant

Can Externally Applied Atp Supply Energy to Active Ion Uptake Mechanisms of Intact Plant Cells?

1974 ◽  
Vol 1 (2) ◽  
pp. 211 ◽  
Author(s):  
U Lüttge ◽  
EV Schöch ◽  
E Ball
Keyword(s):  
External Medium ◽  
Divalent Cations ◽  
Plant Cells ◽  
Bundle Sheath ◽  
Metabolic Inhibition ◽  
Ion Uptake ◽  
Intact Plant ◽  
K Uptake ◽  
Atp Supply ◽  
Uptake Mechanisms

ATP applied in the external medium of leaf slices and bundle-sheath preparations at concentrations between 1.0 and 2.5 mM apparently enhances K+ uptake. However, this effect depends on the presence of Ca2+ or Mg2+ in the medium. ATP is shown to release a Ca2+ or Mg2+ inhibition of K+ uptake. If the Ca2+ or Mg2+ content of the medium is varied and EDTA used to mimic the effect of ATP, it can be demonstrated that the observed effect of ATP is entirely explicable by its chelation of divalent cations. Externally applied ATP has similar relative effects on K+ uptake under control conditions and under conditions where metabolism is inhibited, and although it apparently fully releases metabolic inhibition, these experiments reveal ambiguities which are discussed. It is con- cluded that apparent effects of externally applied ATP may often be indirect. The possibility of specific, i.e. energy-providing, effects can nevertheless not be ruled out in principle, but more sophisti- cated experimentation than found hitherto in the literature is required to demonstrate such effects.

Gene transfer into intact plant cells by electroinjection through cell walls and membranes

Gene ◽  
1986 ◽  
Vol 41 (1) ◽  
pp. 121-124 ◽  
Author(s):  
Morikawa Hiromichi ◽  
Iida Asako ◽  
Matsui Chiaki ◽  
Ikegami Masato ◽  
Yamada Yasuyuki
Keyword(s):  
Gene Transfer ◽  
Cell Walls ◽  
Plant Cells ◽  
Intact Plant

scholarly journals Gold nanocluster mediated delivery of siRNA to intact plant cells for efficient gene knockdown

2021 ◽  
Author(s):  
Huan Zhang ◽  
Yuhong Cao ◽  
Dawei Xu ◽  
Natalie S. Goh ◽  
Gozde S. Demirer ◽  
...  
Keyword(s):  
Target Genes ◽  
Plant Cell Wall ◽  
Sirna Delivery ◽  
Plant Cells ◽  
Intact Plant ◽  
Gene Knockdown ◽  
Size Exclusion ◽  
Plant Host ◽  
Gold Nanocluster ◽  
Efficient Gene

AbstractRNA interference (RNAi), which involves the delivery of small interfering RNA molecules (siRNA), has been used to validate target genes in plants, to understand and control cellular metabolic pathways, and as a ‘green’ alternative for crop pest tolerance. Conventional siRNA delivery methods such as viruses and Agrobacterium-mediated delivery exhibit limitations in host plant species range and their use can result in uncontrolled DNA integration into the plant host genome. Here, we synthesize polyethyleneimine functionalized gold nanoclusters (PEI-AuNCs) to mediate siRNA delivery into intact plant cells and show these constructs enable efficient gene knockdown. We demonstrate that functionalized AuNCs protect siRNA from RNase degradation and are small enough (~2 nm) to bypass the plant cell wall which exhibits a size exclusion limit of 5-20 nm. These AuNCs in turn enable up to 76.5 ± 5.9% GFP mRNA knockdown efficiency with no cellular toxicity. Our data suggest this simple and biocompatible platform for passive delivery of siRNA into intact plant cells could have broad applications in plant biotechnology.

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