scholarly journals Photoinjection of fluorescent nanoparticles into intact plant cells using femtosecond laser amplifier

APL Photonics ◽  
2020 ◽  
Vol 5 (6) ◽  
pp. 066104
Author(s):  
Taufiq Indra Rukmana ◽  
Gabriela Moran ◽  
Rachel Méallet-Renault ◽  
Gilles Clavier ◽  
Tadashi Kunieda ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Plant Cells ◽  
Intact Plant ◽  
Fluorescent Nanoparticles ◽  
Laser Amplifier

scholarly journals Direct observation of nanoparticle diffusion in cytoplasm of single plant cells realized by photoinjection with femtosecond laser amplifier

2020 ◽  
Vol 13 (11) ◽  
pp. 117002
Author(s):  
Taufiq Indra Rukmana ◽  
Ryohei Yasukuni ◽  
Gabriela Moran ◽  
Rachel Méallet-Renault ◽  
Gilles Clavier ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Direct Observation ◽  
Plant Cells ◽  
Single Plant ◽  
Laser Amplifier ◽  
Nanoparticle Diffusion

scholarly journals Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Taufiq Indra Rukmana ◽  
Gabriela Moran ◽  
Rachel Méallet-Renault ◽  
Misato Ohtani ◽  
Taku Demura ◽  
...  
Keyword(s):  
Cell Wall ◽  
Femtosecond Laser ◽  
Mammalian Cells ◽  
Fluorescent Dyes ◽  
Turgor Pressure ◽  
Plant Cells ◽  
Mesh Size ◽  
Enzyme Treatment ◽  
Laser Amplifier ◽  
Mechanical Tensile

AbstractFemtosecond laser photoporation has become a popular method to deliver various kinds of molecules such as genes, proteins, and fluorescent dyes into single mammalian cells. However, this method is not easily applied to plant cells because their cell wall and turgor pressure prevent the delivery, especially for larger molecules than the mesh size of the cell wall. This work is the first demonstration of the efficient photoinjection of megadalton molecules into a cytoplasm of an intact single plant cell by employing a femtosecond laser amplifier under moderate enzyme treatment conditions. The intense femtosecond laser pulse effectively formed a pore on the cell wall and membrane of Tobacco BY-2, and 2 MDa dextran molecules were introduced through the pore. Along with the pore formation, induced mechanical tensile stresses on BY-2 cells were considered to increase permeability of the cell membrane and enhance the uptake of large molecules. Moreover, the moderate enzyme treatment partially degraded the cell wall thereby facilitating the increase of the molecular introduction efficiency.

Photonic Crystal Fiber Femtosecond Laser Amplifier with Millijoules and 100 fs Level Output

2010 ◽  
Vol 37 (9) ◽  
pp. 2415-2418
Author(s):  
刘博文 Liu Bowen ◽  
胡明列 Hu Minglie ◽  
宋有建 Song Youjian ◽  
李毅 Li Yi ◽  
刘华刚 Liu Huagang ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Femtosecond Laser ◽  
Photonic Crystal Fiber ◽  
Laser Amplifier ◽  
Crystal Fiber

scholarly journals Photoporation of Biomolecules into Single Cells in Living Vertebrate Embryos Induced by a Femtosecond Laser Amplifier

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e27677 ◽  
Author(s):  
Yoichiroh Hosokawa ◽  
Haruki Ochi ◽  
Takanori Iino ◽  
Akihiro Hiraoka ◽  
Mikiko Tanaka
Keyword(s):  
Femtosecond Laser ◽  
Single Cells ◽  
Laser Amplifier ◽  
Vertebrate Embryos

scholarly journals Carbon nanocarriers deliver siRNA to intact plant cells for efficient gene knockdown

2020 ◽  
Vol 6 (26) ◽  
pp. eaaz0495 ◽  
Author(s):  
Gozde S. Demirer ◽  
Huan Zhang ◽  
Natalie S. Goh ◽  
Rebecca L. Pinals ◽  
Roger Chang ◽  
...  
Keyword(s):  
Sirna Delivery ◽  
Control Plant ◽  
Plant Cells ◽  
Plant Biotechnology ◽  
Intact Plant ◽  
Intact Cells ◽  
Efficient Gene ◽  
Dna Vectors ◽  
And Control ◽  
Interfering Rna

Posttranscriptional gene silencing (PTGS) is a powerful tool to understand and control plant metabolic pathways, which is central to plant biotechnology. PTGS is commonly accomplished through delivery of small interfering RNA (siRNA) into cells. Standard plant siRNA delivery methods (Agrobacterium and viruses) involve coding siRNA into DNA vectors and are only tractable for certain plant species. Here, we develop a nanotube-based platform for direct delivery of siRNA and show high silencing efficiency in intact plant cells. We demonstrate that nanotubes successfully deliver siRNA and silence endogenous genes, owing to effective intracellular delivery and nanotube-induced protection of siRNA from nuclease degradation. This study establishes that nanotubes could enable a myriad of plant biotechnology applications that rely on RNA delivery to intact cells.

Accommodation of powdery mildew fungi in intact plant cells

2008 ◽  
Vol 165 (1) ◽  
pp. 5-18 ◽  
Author(s):  
Ruth Eichmann ◽  
Ralph Hückelhoven
Keyword(s):  
Powdery Mildew ◽  
Plant Cells ◽  
Intact Plant ◽  
Powdery Mildew Fungi

Plasma Cleaning of Compressed Grating in Chirped-Pulse Femtosecond Laser Amplifier

2012 ◽  
Vol 39 (4) ◽  
pp. 0402006
Author(s):  
葛绪雷 Ge Xulei ◽  
滕浩 Teng Hao ◽  
郑轶 Zheng Yi ◽  
马景龙 Ma Jinglong ◽  
张伟 Zhang Wei ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Plasma Cleaning ◽  
Laser Amplifier ◽  
Chirped Pulse

Femtosecond optical injection of intact plant cells using a reconfigurable platform

2014 ◽  
Author(s):  
Claire A. Mitchell ◽  
Stefan Kalies ◽  
Tomas Cizmar ◽  
Nicola Bellini ◽  
Anisha Kubasik-Thayil ◽  
...  
Keyword(s):  
Plant Cells ◽  
Optical Injection ◽  
Intact Plant ◽  
Reconfigurable Platform

scholarly journals Nanotubes Effectively Deliver siRNA to Intact Plant Cells and Protect siRNA Against Nuclease Degradation

2019 ◽  
Author(s):  
Gozde Demirer ◽  
Huan Zhang ◽  
Natalie Goh ◽  
Roger Chang ◽  
Markita Landry
Keyword(s):  
Plant Cells ◽  
Intact Plant ◽  
Nuclease Degradation
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